Polymerase chain reaction and direct sequencing of Neisseria gonorrhoeae protein IB gene: partial nucleotide and amino acid sequence analysis of strains S4, S11, S48 (serovar IB4) and S34 (serovar IB5)

1993 ◽  
Vol 182 (3) ◽  
Author(s):  
Q.C. Lau ◽  
V.T.K. Chow ◽  
C.L. Poh
Keyword(s):  
Polymerase Chain Reaction ◽  
Amino Acid ◽  
Sequence Analysis ◽  
Amino Acid Sequence ◽  
Neisseria Gonorrhoeae ◽  
Direct Sequencing ◽  
Amino Acid Sequence Analysis ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Human FLT3/FLK2 gene: cDNA cloning and expression in hematopoietic cells

Blood ◽  
1993 ◽  
Vol 82 (4) ◽  
pp. 1110-1119 ◽  
Author(s):  
O Rosnet ◽  
C Schiff ◽  
MJ Pebusque ◽  
S Marchetto ◽  
C Tonnelle ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Amino Acid ◽  
Amino Acid Sequence ◽  
Tyrosine Kinase ◽  
B Cell ◽  
Cloning And Expression ◽  
Colony Stimulating Factor ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Stimulating Factor

The human FLT3 cDNA was cloned from a pre-B cell line and characterized. The deduced amino acid sequence shows that FLT3 codes for a receptor-type tyrosine kinase of 993 residues, presenting a strong similarity with the corresponding mouse FLT3/FLK2 protein as well as with the receptors for colony-stimulating factor 1 (CSF1R/FMS) and steel locus factor (SLFR/KIT). An analysis of the expression of the gene using amplification of reverse transcribed FLT3 mRNA by polymerase chain reaction shows that FLT3 is expressed in various lymphohematopoietic cells and tissues, including a series of immature cell lines and leukemias of lymphocytic origin.

scholarly journals Sequence of the S10 cDNA from 'McIntosh' Apple and a PCR-digestion Identification Method

HortScience ◽  
2002 ◽  
Vol 37 (1) ◽  
pp. 187-190 ◽  
Author(s):  
Kentaro Kitahara ◽  
Shogo Matsumoto
Keyword(s):  
Polymerase Chain Reaction ◽  
Amino Acid ◽  
Amino Acid Sequence ◽  
Restriction Enzyme ◽  
Chain Reaction ◽  
Specific Primers ◽  
Amino Acid Sequence Level ◽  
Identification Method ◽  
Polymerase Chain

An S-allele cDNA was cloned from pistils of 'McIntosh' apple (Malus ×domestica Borkh.). The allele, designated Si in Japan and S10 in Europe, is an S-RNase that is very similar (94%) to the S3-RNase at the deduced amino acid sequence level. This allele can be detected by amplification using the polymerase chain reaction (PCR) and specific primers, followed by digestion with restriction enzyme EheI. The S10 allele was discovered in 'Empire', 'Maypole', 'Shinano Red', 'Spencer', and 'Vista Bella'. The S-allele cDNAs sequenced to date are listed with their Japanese and European designations.

Cloning and sequencing of a cDNA encoding an ovine oestrus-associated oviducal protein

1996 ◽  
Vol 8 (2) ◽  
pp. 305 ◽  
Author(s):  
JT Marshall ◽  
CD Nancarrow ◽  
AG Brownlee
Keyword(s):  
Amino Acids ◽  
Polymerase Chain Reaction ◽  
Amino Acid ◽  
Amino Acid Sequence ◽  
Northern Blot ◽  
Mature Protein ◽  
Chain Reaction ◽  
Cloning And Sequencing ◽  
Glycosylation Sites ◽  
Polymerase Chain

Ovine oestrus-associated oviducal glycoprotein (oEGP) is synthesized and secreted specifically by the ampullary region of the ovine oviduct during the peri-ovulatory stages of the oestrous cycle. A cDNA that encodes oEGP was isolated and sequenced. Isolation of oEGP was achieved using the polymerase chain reaction (PCR) with primers based on a bovine oestrus-associated oviducal glycoprotein cDNA (bOGP) sequence. A 1599-bp cDNA encodes, in part, a deduced 519-amino acid sequence of mature protein which carries two potential N-linked glycosylation sites. The deduced amino acid sequence is more than 95% identical to that of bOGP and more than 74% identical to the first 491 amino acids of human oestrogen-dependent oviducal glycoprotein (hOGP). Northern blot hybridizations of RNA from several sheep tissues detected mRNA (2.4 kb) only in an ampulla oviduct sample.

scholarly journals Analysis of N-RAS exon-1 mutations in myelodysplastic syndromes by polymerase chain reaction and direct sequencing

Blood ◽  
1989 ◽  
Vol 73 (1) ◽  
pp. 281-283 ◽  
Author(s):  
M Bar-Eli ◽  
H Ahuja ◽  
N Gonzalez-Cadavid ◽  
A Foti ◽  
MJ Cline
Keyword(s):  
Polymerase Chain Reaction ◽  
Amino Acid ◽  
Aspartic Acid ◽  
Gene Amplification ◽  
Myelodysplastic Syndromes ◽  
Direct Sequencing ◽  
Chain Reaction ◽  
Ras Gene ◽  
Exon 1 ◽  
Polymerase Chain

Abstract Mutations in codons 12 or 13 of the first exon of the N-RAS gene have been reported in myelodysplastic syndromes (MDS) in frequencies that vary between 9% and 40% depending on the techniques used in analysis. Gene amplification and direct sequencing provides the only unambiguous method of detecting those mutations that induce amino acid alterations. Using this technique, we analyzed 21 MDS patients for mutations in exon- 1 of N-RAS. Codon 12 mutations substituting aspartic acid (GAT) for glycine (GGT) were found in four cases, and a codon 13 mutation substituting alanine (GCT) for glycine (GGT) was detected in one patient. We conclude that N-RAS exon-1 mutations in one patient. We conclude that N-RAS exon-1 mutations producing amino acid changes occur in about 20% to 25% of MDS cases.

scholarly journals A New S-allele in Apple, `Sg', and Its Similarity to the `Sf' Allele from `Fuji'

HortScience ◽  
1999 ◽  
Vol 34 (4) ◽  
pp. 708-710 ◽  
Author(s):  
Shogo Matsumoto ◽  
Kentaro Kitahara ◽  
Sadao Komori ◽  
Junichi Soejima
Keyword(s):  
Polymerase Chain Reaction ◽  
Amino Acid ◽  
Nucleotide Sequence ◽  
Amino Acid Sequence ◽  
Rflp Analysis ◽  
Chain Reaction ◽  
Specific Polymerase Chain Reaction ◽  
Allele Specific ◽  
Polymerase Chain ◽  
S Allele

S-allele genotypes of nine apple (Malus ×domestica Borkh.) cultivars were identified using S-allele–specific polymerase chain reaction (PCR)–restriction fragmentlength polymorphism (RFLP) analysis. A new S-allele, Sg, was proposed to be present in `American Summer Pearmain', `Indo', `Kitanosachi', and `Meku 10'. This allele is very similar to Sf at the nucleotide sequence (92%) and deduced amino acid sequence (94%) levels.

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