Computer graphic three-dimensional reconstruction of normal human embryo morphogenesis

1996 ◽  
Vol 194 (5) ◽  
Author(s):  
G.A. Machin ◽  
G.H. Sperber ◽  
I. Ongaro ◽  
C. Murdoch
Keyword(s):  
Human Embryo ◽  
Computer Graphic ◽  
Three Dimensional ◽  
Three Dimensional Reconstruction ◽  
Dimensional Reconstruction ◽  
Normal Human

Narrowest (ISTHMUS) Portion of Eustachian Tube: A Computer-Aided Three-Dimensional Reconstruction and Measurement Study

1997 ◽  
Vol 106 (7) ◽  
pp. 583-588 ◽  
Author(s):  
Masaharu Sudo ◽  
Isamu Sando ◽  
Akihiro Ikui ◽  
Chiaki Suzuki
Keyword(s):  
Eustachian Tube ◽  
Middle Ear ◽  
Three Dimensional ◽  
Three Dimensional Reconstruction ◽  
Cross Sectional ◽  
Computer Aided ◽  
Dimensional Reconstruction ◽  
Tensor Veli Palatini Muscle ◽  
Normal Human ◽  
Temporal Bones

Nine normal human temporal bones from persons 16 to 88 years old were studied by computer aided three-dimensional reconstruction and measurement. The length of the eustachian tube (ET) lumen in three portions (from pharyngeal orifice to tympanic orifice: cartilaginous, junctional, and bony) averaged 23.6 ±4.3 mm, 3.0 ± 1.9 mm, and 6.4 ± 2.6 mm. The narrowest portion of the ET lumen was in the cartilaginous portion in all cases: 20.5 ± 4.2 mm from the pharyngeal orifice and 3.1 ± 1.6 mm from the pharyngeal margin of the junctional portion. The cross-sectional area of the narrowest portion was 0.65 ± 0.2 mm2. The tendon of the tensor veli palatini muscle (TVPM) inserted into the lateral lamina in the narrowest portion of the ET lumen in five of nine cases. These results suggest that contraction of the TVPM opens the narrowest portion of the ET lumen to ventilate the middle ear and that this portion also plays a role in protecting the middle ear.

Postnatal Development of the Internal Auditory Canal Studied by Computer-Aided Three-Dimensional Reconstruction and Measurement

1995 ◽  
Vol 104 (6) ◽  
pp. 469-475 ◽  
Author(s):  
Tetsushi Sakashita ◽  
Isamu Sando
Keyword(s):  
Postnatal Development ◽  
Three Dimensional ◽  
Internal Auditory Canal ◽  
First Year ◽  
Three Dimensional Reconstruction ◽  
Middle Portion ◽  
Computer Aided ◽  
Dimensional Reconstruction ◽  
Normal Human ◽  
Temporal Bones

Postnatal development of the internal auditory canal (IAC) was investigated in 20 normal human temporal bones obtained from individuals 1 month to 72 years old. Computer-aided three-dimensional reconstruction and measurement of bones showed that the superior, inferior, anterior, and posterior walls of the IAC lengthen significantly from birth until about 10 years of age, with development mainly attributable to lengthening of the part of the IAC medial to the foramen singulare. The lengths of the part of the IAC lateral to the foramen singulare and of the transverse crest and Bill's bar did not appear to develop postnatally. The IAC diameter increased slightly at the porus for about the first year after birth, but not at the fundus or the middle portion of the canal. This finding was confirmed by studying the shape of the IAC. Postnatal increases in the volume of the IAC followed patterns similar to that of increases in length of the IAC walls. These results show that postnatally the IAC increases significantly in length until about 10 years of age and slightly in diameter until about 1 year of age, especially medial to the foramen singulare. This concentration of growth of the IAC medially implies that its postnatal development is mainly due to growth of the bone around the otic capsule, which has implications for IAC surgery.

Three-Dimensional Reconstruction of Cutaneous Arteries of the Back by Computer Graphic Imaging

1997 ◽  
Vol 100 (2) ◽  
pp. 381-389 ◽  
Author(s):  
Hideo Nakajima ◽  
Nobuaki Imanishi ◽  
Toshiharu Minabe ◽  
Toyomi Fujino ◽  
Sadakazu Aiso
Keyword(s):  
Computer Graphic ◽  
Three Dimensional ◽  
Three Dimensional Reconstruction ◽  
Dimensional Reconstruction

Methods for three-dimensional reconstruction of cellular components within a thick section

1986 ◽  
Vol 44 ◽  
pp. 18-21
Author(s):  
J. Frank ◽  
B. F. McEwen ◽  
M. Radermacher ◽  
C. L. Rieder
Keyword(s):  
Tilt Angle ◽  
Tomographic Reconstruction ◽  
3D Structure ◽  
Three Dimensional ◽  
Thick Section ◽  
Three Dimensional Reconstruction ◽  
Axis Ratio ◽  
Dimensional Reconstruction ◽  
Cellular Components

The tomographic reconstruction from multiple projections of cellular components, within a thick section, offers a way of visualizing and quantifying their three-dimensional (3D) structure. However, asymmetric objects require as many views from the widest tilt range as possible; otherwise the reconstruction may be uninterpretable. Even if not for geometric obstructions, the increasing pathway of electrons, as the tilt angle is increased, poses the ultimate upper limitation to the projection range. With the maximum tilt angle being fixed, the only way to improve the faithfulness of the reconstruction is by changing the mode of the tilting from single-axis to conical; a point within the object projected with a tilt angle of 60° and a full 360° azimuthal range is then reconstructed as a slightly elliptic (axis ratio 1.2 : 1) sphere.

Three-Dimensional Reconstruction of Native Androctonus Australis Hemocyanin

1990 ◽  
Vol 48 (1) ◽  
pp. 452-453
Author(s):  
Nicolas Boisset ◽  
Jean-Christophe Taveau ◽  
Jean Lamy ◽  
Terence Wagenknecht ◽  
Michael Radermacher ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Solid Body ◽  
Body Surface ◽  
Three Dimensional ◽  
Staining Technique ◽  
Three Dimensional Reconstruction ◽  
Dimensional Reconstruction ◽  
View Reconstruction ◽  
Surface Representations ◽  
Top View

Hemocyanin, the respiratory pigment of the scorpion Androctonus australis is composed of 24 kidney shaped subunits. A model of architecture supported by many indirect arguments has been deduced from electron microscopy (EM) and immuno-EM. To ascertain, the disposition of the subunits within the oligomer, the 24mer was submitted to three-dimensional reconstruction by the method of single-exposure random-conical tilt series.A sample of native hemocyanin, prepared with the double layer negative staining technique, was observed by transmisson electron microscopy under low-dose conditions. Six 3D-reconstructions were carried out indenpendently from top, side and 45°views. The results are composed of solid-body surface representations, and slices extracted from the reconstruction volume.The main two characters of the molecule previously reported by Van Heel and Frank, were constantly found in the solid-body surface representations. These features are the presence of two different faces called flip and flop and a rocking of the molecule around an axis passing through diagonnally opposed hexamers. Furthermore, in the solid-body surface of the top view reconstruction, the positions and orientations of the bridges connecting the half molecules were found in excellent agreement with those predicted by the model.

Three-Dimensional Reconstruction of Two Forms of the Chaperonin GRO EL

1990 ◽  
Vol 48 (1) ◽  
pp. 258-259
Author(s):  
J.L. Carrascosa ◽  
G. Abella ◽  
S. Marco ◽  
M. Muyal ◽  
J.M. Carazo
Keyword(s):  
Three Dimensional ◽  
The Other ◽  
Two Dimensional ◽  
Series Method ◽  
Three Dimensional Reconstruction ◽  
Structural Components ◽  
E Coli ◽  
Dimensional Reconstruction ◽  
Morphogenetic Factors ◽  
Tilt Series

Chaperonins are a class of proteins characterized by their role as morphogenetic factors. They trantsiently interact with the structural components of certain biological aggregates (viruses, enzymes etc), promoting their correct folding, assembly and, eventually transport. The groEL factor from E. coli is a conspicuous member of the chaperonins, as it promotes the assembly and morphogenesis of bacterial oligomers and/viral structures.We have studied groEL-like factors from two different bacteria:E. coli and B.subtilis. These factors share common morphological features , showing two different views: one is 6-fold, while the other shows 7 morphological units. There is also a correlation between the presence of a dominant 6-fold view and the fact of both bacteria been grown at low temperature (32°C), while the 7-fold is the main view at higher temperatures (42°C). As the two-dimensional projections of groEL were difficult to interprete, we studied their three-dimensional reconstruction by the random conical tilt series method from negatively stained particles.

247: Three Dimensional Reconstruction of Nervature in Prostate: Confirming Cancer Related Axonogenesis

2006 ◽  
Vol 175 (4S) ◽  
pp. 82-82
Author(s):  
Gustavo Ayala ◽  
Rile Li ◽  
Hong Oai ◽  
Mohammad Sayeeddudin ◽  
Timothy C. Thompson ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Three Dimensional Reconstruction ◽  
Dimensional Reconstruction
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