Some aspects of hybridoma cell cultivation

1991 ◽  
Vol 35 (2) ◽  
Author(s):  
H. Graf ◽  
K. Sch�gerl
Keyword(s):  
Cell Cultivation

Human liver cell cultivation for long-term in vitro toxicity studies in a miniaturized multi-compartment 3D bioreactor system

2011 ◽  
Vol 49 (01) ◽  
Author(s):  
SA Hoffmann ◽  
M Lübberstedt ◽  
U Müller-Vieira ◽  
D Knobeloch ◽  
A Nüssler ◽  
...  
Keyword(s):  
Liver Cell ◽  
Human Liver ◽  
In Vitro Toxicity ◽  
Cell Cultivation ◽  
Toxicity Studies ◽  
Human Liver Cell ◽  
Bioreactor System

The mystery behind the nostrils – technical clues for successful nasal epithelial cell cultivation

2021 ◽  
pp. 151748
Author(s):  
Malik Aydin ◽  
Ella A. Naumova ◽  
Aliyah Bellm ◽  
Ann-Kathrin Behrendt ◽  
Federica Giachero ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Cultivation ◽  
Nasal Epithelial Cell

scholarly journals Cellular Orientation on Repeatedly Stretching Gelatin Hydrogels with Supramolecular Cross-Linkers

Polymers ◽  
2019 ◽  
Vol 11 (12) ◽  
pp. 2095
Author(s):  
Dae Hoon Lee ◽  
Yoshinori Arisaka ◽  
Asato Tonegawa ◽  
Tae Woong Kang ◽  
Atsushi Tamura ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Chemical Composition ◽  
Cellular Response ◽  
Linear Polymer ◽  
Cross Linking ◽  
Adherent Cells ◽  
Cell Cultivation ◽  
Supramolecular Compound ◽  
Synthetic Materials ◽  
Cyclic Molecules

The cytocompatibility of biological and synthetic materials is an important issue for biomaterials. Gelatin hydrogels are used as biomaterials because of their biodegradability. We have previously reported that the mechanical properties of gelatin hydrogels are improved by cross-linking with polyrotaxanes, a supramolecular compound composed of many cyclic molecules threaded with a linear polymer. In this study, the ability of gelatin hydrogels cross-linked by polyrotaxanes (polyrotaxane–gelatin hydrogels) for cell cultivation was investigated. Because the amount of polyrotaxanes used for gelatin fabrication is very small, the chemical composition was barely altered. The structure and wettability of these hydrogels are also the same as those of conventional hydrogels. Fibroblasts adhered on polyrotaxane–gelatin hydrogels and conventional hydrogels without any reduction or apoptosis of adherent cells. From these results, the polyrotaxane–gelatin hydrogels have the potential to improve the mechanical properties of gelatin without affecting cytocompatibility. Interestingly, when cells were cultured on polyrotaxane–gelatin hydrogels after repeated stress deformation, the cells were spontaneously oriented to the stretching direction. This cellular response was not observed on conventional hydrogels. These results suggest that the use of a polyrotaxane cross-linking agent can not only improve the strength of hydrogels but can also contribute to controlling reorientation of the gelatin.

Disposable 600-mL orbitally shaken bioreactor for mammalian cell cultivation in suspension

2013 ◽  
Vol 76 ◽  
pp. 6-12 ◽  
Author(s):  
Dominique T. Monteil ◽  
Giulia Tontodonati ◽  
Saroj Ghimire ◽  
Lucia Baldi ◽  
David L. Hacker ◽  
...  
Keyword(s):  
Mammalian Cell ◽  
Cell Cultivation ◽  
Mammalian Cell Cultivation

scholarly journals Contactless Investigations of Yeast Cell Cultivation in the 7 GHz and 240 GHz Ranges

2013 ◽  
Vol 434 ◽  
pp. 012033 ◽  
Author(s):  
J Wessel ◽  
K Schmalz ◽  
G Gastrock ◽  
B P Cahill ◽  
C Meliani
Keyword(s):  
Yeast Cell ◽  
Cell Cultivation

scholarly journals Hypoxia-on-a-chip

2016 ◽  
Vol 2 (1) ◽  
pp. 71-75 ◽  
Author(s):  
Mathias Busek ◽  
Stefan Grünzner ◽  
Tobias Steege ◽  
Udo Klotzbach ◽  
Frank Sonntag
Keyword(s):  
Oxygen Transfer ◽  
Chemical Resistance ◽  
Cell Cultivation ◽  
Active Components ◽  
Pure Nitrogen ◽  
Micro Pump ◽  
Before And After ◽  
Process Gases ◽  
Fluorescence Lifetime Detection ◽  
Sufficient Oxygen

AbstractIn this work a microfluidic cell cultivation device for perfused hypoxia assays as well as a suitable controlling unit are presented. The device features active components like pumps for fluid actuation and valves for fluid direction as well as an oxygenator element to ensure a sufficient oxygen transfer. It consists of several individually structured layers which can be tailored specifically to the intended purpose. Because of its clearness, its mechanical strength and chemical resistance as well as its well-known biocompatibility polycarbonate was chosen to form the fluidic layers by thermal diffusion bonding. Several oxygen sensing spots are integrated into the device and monitored with fluorescence lifetime detection. Furthermore an oxygen regulator module is implemented into the controlling unit which is able to mix different process gases to achieve a controlled oxygenation. First experiments show that oxygenation/deoxygenation of the system is completed within several minutes when pure nitrogen or air is applied to the oxygenator. Lastly the oxygen input by the pneumatically driven micro pump was quantified by measuring the oxygen content before and after the oxygenator.

Anchorage-Dependent Cell Cultivation

2013 ◽  
Vol 33 (12) ◽  
pp. 36-37
Author(s):  
Yas Hashimura ◽  
Daniel Giroux ◽  
Brian Lee
Keyword(s):  
Cell Cultivation ◽  
Dependent Cell

Bioreactor Design for Insect Cell Cultivation: A Review

2020 ◽  
pp. 51-68
Author(s):  
Janusz J. Malinowski ◽  
Andrew J. Daugulis
Keyword(s):  
Insect Cell ◽  
Cell Cultivation ◽  
Bioreactor Design
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