Comparison of bromodeoxyuridine uptake and MIB 1 immunoreactivity in medulloblastomas determined with single and double immunohistochemical staining methods

1996 ◽  
Vol 29 (2) ◽  
Author(s):  
Kiyoshi Onda ◽  
RichardL. Davis ◽  
MichaelS.B. Edwards
Keyword(s):  
Immunohistochemical Staining ◽  
Staining Methods ◽  
Double Immunohistochemical Staining ◽  
Mib 1

scholarly journals Immunohistochemical Markers and the Clinical Course of Adenosarcoma: a Series of Seven Cases

2020 ◽  
Author(s):  
Makiko Omi ◽  
Akiko Tonooka ◽  
Tomohiro Chiba ◽  
Hidetaka Nomura ◽  
Hiroyuki Kanao ◽  
...  
Keyword(s):  
Immunohistochemical Staining ◽  
Clinical Course ◽  
Abnormal Uterine Bleeding ◽  
P53 Expression ◽  
Prognostic Assessment ◽  
Immunohistochemical Markers ◽  
Recurrent Tumors ◽  
Gynecology And Obstetrics ◽  
Staining Methods ◽  
The Relationship

Abstract Background: Uterine adenosarcoma, a rare subtype of uterine tumor, is a biphasic tumor consisting of epithelial and mesenchymal elements. There is no research comparing histopathological features of primary and recurrent tumors, including immunohistochemistry; furthermore, the relationship between pathology and the clinical course is unclear. We reviewed the pathology and immunohistochemical features of 7 adenosarcoma cases and investigated the relevance of the histomorphological features to the clinical course. We also compared immunohistochemical features of primary and recurrent tumors.Methods: Seven patients with adenosarcoma who underwent surgery in our hospital were evaluated. We performed immunohistochemical staining for the estrogen receptor (ER), progesterone receptor (PR), p53, and two SWI/SNF chromatin-remodeling proteins (SMARCA4, BCOR), which were recently developed for the diagnosis of undifferentiated sarcomas in addition to conventional staining methods. Results: All patients were International Federation of Gynecology and Obstetrics stage 1B to 1C. All tumors were polypoid, and every patient presented with abnormal uterine bleeding. Six patients were over 50 years old and were menopausal; 1 patient was under 50 years old and was non-menopausal (average age 59.1 years). Histologically, the sarcomatous components were homologous in 6 patients and heterogenous in 1 patient. Four patients were recurrent patients; 3 were non-recurrent. All 4 recurrent patients showed high-grade morphology with sarcomatous overgrowth and were negative for ER and PR. Three recurrences could be evaluated by imaging, showing recurrence only in a distant area; biopsy specimens from these tissues revealed the identical mesenchymal component found in the primary tumor without a benign epithelial component. Immunohistochemical staining results were also the same as for the original tumor, except for the p53 expression in 1 patient. At the primary site, p53 was overexpressed in 2 recurrent patients and had a wild-type level in 1 recurrent patient; however, all 3 recurrent tissues showed overexpression of p53. None of the 7 cases showed SMARCA4 loss, and BCOR expression was positive in 1 case.Conclusions: Initial pathological analysis of the adenosarcoma with appropriate immunohistochemical staining is vital for prognostic assessment. Expression of p53 might increase at recurrence. SMARCA4 and BCOR could be an index of malignancy, regardless of sarcomatous overgrowth.

scholarly journals Oral Pseudolymphoma: A Report of 2 Cases

2014 ◽  
Vol 18 (1) ◽  
pp. 48-51
Author(s):  
Erdogan Fisekcioglu ◽  
Semih Ozbayrak ◽  
Gulcin Erseven ◽  
Vakur Olgac
Keyword(s):  
Differential Diagnosis ◽  
Oral Cavity ◽  
Light Microscope ◽  
Immunohistochemical Staining ◽  
Staining Methods

Abstract Objectives: The aim of this study was to present 2 infrequent cases pseudolymphoma in the oral cavity, to discuss their locations and to emphasize the importance of differential diagnosis for the prognosis. Methods: Excisional biopsies were performed in a 34 year-old women complaining for erythematous lesion in the oral floor and in a 44 year-old man who had a bluish lesion and erythema in the oral floor. Both specimens were investigated routinely and CD3, CD20, CD45, CD23, CD5 and FVIII markers. Immunohistochemical staining methods was applied and investigation was performed under light microscope. After the investigation, the cases were diagnosed as pseudolymphomas. Results: Pseudolymphoma may show clinical and histologic resemblance to lymphoma, so that differantiation with lymphoma can be very difficult. Conclusion: Pseudolymphomas have a tendency to malign differantiation; these differences must be evaluated clinically.

Proliferation of endothelial cells in neoangiogenesis of human cortical glioblastoma

2008 ◽  
Vol 3 (2) ◽  
pp. 113-119
Author(s):  
Mira Krstulja ◽  
David Bonifacic ◽  
Slobodan Cvejanović
Keyword(s):  
Cell Proliferation ◽  
Endothelial Cells ◽  
Endothelial Cell ◽  
Immunohistochemical Staining ◽  
Endothelial Cell Proliferation ◽  
Specific Cell ◽  
Medical Doctors ◽  
Close Proximity ◽  
Double Immunohistochemical Staining ◽  
First Time

AbstractIn 1982 Vilanova et al. quantitatively described the neovessel area in glioblastoma and suggested zonal differences in vessel surface area. In this study we investigated specific cell proliferation and angiogenic patterns in the vessel compartment of cortical glioblastoma. We used Ki67, CD34 and SMA double immunohistochemical staining to quantitate vascular patterns and cell specific proliferation and presented glioblastoma with several parameters of angiogenesis. Endothelial cell proliferation was higher in complex and bizzare neovessels than in the simple and sprouting glioblastoma neovessels. There was a higher frequency of sprouting simple vessels in close proximity to the palisade and a higher frequency of bizzare vessels in the microzone distant to the palisade. Quantitatively presented for the first time, the neovessel proliferation patterns support cortical glioblastoma compartmentalization. The data obtained are relevant to medical doctors using neoangiogenesis in diagnosis, prognosis and therapy of neoplasia. The results obtained in 15 patients call for further investigation of endothelial cell/pericyte relationships and glioblastoma compartmentalization.

Results of immunohistochemical staining for cell cycle regulators predict the recurrence of atypical meningiomas

2014 ◽  
Vol 121 (5) ◽  
pp. 1189-1200 ◽  
Author(s):  
Min Soo Kim ◽  
Kyu Hong Kim ◽  
Eun Hee Lee ◽  
Young Min Lee ◽  
Sung-Hun Lee ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cyclin D1 ◽  
Immunohistochemical Staining ◽  
Regulatory Proteins ◽  
Incomplete Resection ◽  
Presenting Symptoms ◽  
Cell Cycle Regulatory Proteins ◽  
Atypical Meningiomas ◽  
Mib 1

Object The aim of this study was to evaluate the role of certain cell-cycle regulatory proteins in the recurrence of atypical meningiomas. These proteins were analyzed with immunohistochemical staining to identify predisposing factors for the recurrence of atypical meningiomas. Methods The authors retrospectively reviewed the medical records of patients with atypical meningiomas diagnosed in the period from January 2000 to June 2012 at the Department of Neurosurgery at Samsung Changwon Hospital and Dong-A University Medical Center. Clinical data included patient sex and age at the time of surgery, presenting symptoms at diagnosis, location and size of tumor, extent of surgery, use of postoperative radiotherapy, duration of follow-up, and recurrence. Immunohistochemical staining for cell-cycle regulatory proteins (p16, p15, p21, p27, cyclin-dependent kinase [CDK] 4 and 6, phosphorylated retinoblastoma [pRB] protein, and cyclin D1) and proliferative markers (MIB-1 antigen, mitosis, and p53) was performed on archived paraffin-embedded tissues obtained during resection. The recurrence rate and time to recurrence were assessed using Kaplan-Meier analysis. Results Of the 67 atypical meningiomas eligible for analysis, 26 (38.8%) recurred during the follow-up period (mean duration 47.7 months, range 8.4–132.1 months). Immunohistochemically, there was overstaining for p16 in 44 samples (65.7%), for p15 in 21 samples (31.3%), for p21 in 25 samples (37.3%), for p27 in 32 samples (47.8%), for CDK4 in 38 samples (56.7%), for CDK6 in 26 samples (38.8%), for pRB protein in 42 samples (62.7%), and for cyclin D1 in 49 samples (73.1%). Multivariate analysis using the Cox proportional-hazards regression model showed that incomplete resection (HR 4.513, p < 0.001); immunohistochemical understaining for p16 (HR 3.214, p < 0.001); immunohistochemical overstaining for CDK6 (HR 3.427, p < 0.001), pRB protein (HR 2.854, p = 0.008), and p53 (HR 2.296, p = 0.040); and increased MIB-1 labeling index (HR 2.665, p = 0.013) and mitotic index (HR 2.438, p = 0.024) predicted the recurrence of atypical meningiomas after resection. Conclusions Findings in this study indicated that p16, CDK6, and pRB protein were associated with the recurrence of atypical meningiomas.

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