In vitro growth and collagen synthesis in fibroblasts from the rabbit middle ear mucosa

1994 ◽  
Vol 251 (5) ◽  
Author(s):  
T. Ovesen ◽  
M. Gaihede ◽  
T. Ledet
Keyword(s):  
Middle Ear ◽  
Collagen Synthesis ◽  
In Vitro Growth ◽  
Middle Ear Mucosa

Tissue Culture of Human Adult Adenoids and of Middle Ear Mucosa

1976 ◽  
Vol 85 (3) ◽  
pp. 327-333 ◽  
Author(s):  
Ilana Drucker ◽  
Ziva Weisman ◽  
Jacob Sadé
Keyword(s):  
Middle Ear ◽  
Ciliary Activity ◽  
Organ Cultures ◽  
Middle Ear Mucosa ◽  
Monolayer Growth ◽  
Stratified Squamous Epithelium ◽  
Inducing Factors ◽  
Developmental Characteristics ◽  
Mucociliary Epithelia

The increased number of mucus producing cells as well as the presence of stratified squamous epithelium in pathological and experimental middle ear conditions, point towards the possibility of metaplastic changes of the middle ear mucosa, similar to the metaplastic capabilities of respiratory mucosae in general, as observed clinically or provoked experimentally. The purpose of this study was to develop a model of postembryonic human respiratory mucosae, in vitro, for the study of triggering or inducing factors involved in its normal and metaplastic differentiation. Explants from adenoids and middle ear mucosa were cultured, both as organ cultures and monolayers, for periods of up to two weeks, and their developmental characteristics were studied and described. Over 50% of the explants showed mitosis, epithelial and monolayer growth, ciliary activity and differentiation into ciliated and into mucus-producing cells. Adenoid explants were grown in air without and with added 5% CO2. Under the latter conditions, the proportion of explants and monolayers showing ciliary activity was 50% greater. It is concluded that this model might be suitable for further studies of the factors which control cyto-differentiation in mucociliary epithelia. Maintaining its growth for a longer period would, however, be desirable.

scholarly journals Both canonical and non-canonical NF-κB activation contribute to the proliferative response of the middle ear mucosa during bacterial infection

2016 ◽  
Vol 22 (8) ◽  
pp. 626-634 ◽  
Author(s):  
Chang Gun Cho ◽  
Kwang Pak ◽  
Nicholas Webster ◽  
Arwa Kurabi ◽  
Allen F Ryan
Keyword(s):  
Middle Ear ◽  
Time Course ◽  
Tissue Growth ◽  
Bacterial Disease ◽  
Dependent Manner ◽  
Middle Ear Mucosa ◽  
Mucosal Hyperplasia ◽  
Expression Of Genes ◽  
Canonical Pathways

A major aspect of pathology in otitis media (OM), the most common childhood bacterial disease, is hyperplasia of the middle ear mucosa. Activation of innate immune receptors during OM leads to the activation of NF-κB, a pleiotropic transcription factor involved both in inflammation and tissue growth. To explore the role of NF-κB in mucosal hyperplasia during OM, we evaluated the expression of genes involved in two modes of NF-κB activation during a complete episode of acute, bacterial OM in mice. We also determined the effects of inhibitors of each pathway on infection-stimulated mucosal growth in vitro. A majority of the genes that mediate both the canonical and the non-canonical pathways of NF-κB activation were regulated during OM, many with kinetics related to the time course of mucosal hyperplasia. Inhibition of either pathway reduced the growth of cultured mucosal explants in a dose-dependent manner. However, inhibition of the canonical pathway produced a greater effect, suggesting that this mode of NF-κB activation dominates mucosal hyperplasia during OM.

The effect of N-acetylcysteine on the in vitro growth of normal rabbit middle ear fibroblasts

1993 ◽  
Vol 18 (5) ◽  
pp. 400-405 ◽  
Author(s):  
THERESE OVESEN ◽  
MICHAEL GAIHEDE ◽  
THOMAS LEDET
Keyword(s):  
Middle Ear ◽  
In Vitro Growth ◽  
Middle Ear Fibroblasts

Ciliary beat frequency of human middle-ear mucosa measured in vitro

1984 ◽  
Vol 98 (9) ◽  
pp. 853-856 ◽  
Author(s):  
Y. Roth ◽  
E. Ostfeld
Keyword(s):  
Middle Ear ◽  
Beat Frequency ◽  
Normal Subjects ◽  
Ciliary Beat Frequency ◽  
Fiber Optic Probe ◽  
Middle Ear Mucosa ◽  
Ventilation Tubes ◽  
Optic Probe ◽  
Ciliary Beat

AbstractBrush biopsies from the middle-ear (ME) and nasal mucosa were taken from a 4-year-old girl with complete situs inversus, recurrent otitis media and tonsillitis, who underwent tonsillectomy, adenoidectomy and bilateral myringotomy with insertion of ventilation tubes. Ciliary beat frequencies (CBF, Hz.) of the ME and nasal samples, measured by a photoelectric system equipped with a fiber-optic probe, were 9.5 Hz. (±1.2) and 13.7 Hz. (±4.1) respectively. These values are similar to CBF of nasal samples obtained from normal subjects. In vitro CBF assessment is a useful investigative tool for the study of mucociliary activity in the ME, as in patients suspected to have ciliary dyskinesia syndrome.

Solubilization of keratin debris in conservative treatment of middle ear cholesteatoma: an in vitro study

1994 ◽  
Vol 108 (2) ◽  
pp. 113-115 ◽  
Author(s):  
Kiyofumi Gyo ◽  
Yasushi Sasaki
Keyword(s):  
Hydrogen Peroxide ◽  
Clinical Practice ◽  
Conservative Treatment ◽  
Middle Ear ◽  
In Vitro Study ◽  
Liquid Soap ◽  
Plant Oil ◽  
Middle Ear Mucosa ◽  
Middle Ear Cholesteatoma

AbstractA variety of solutions were tested in vitro to find a suitable solvent which could be used in clinical practice for cholesteatoma debris. Though a little weak as a solvent, a liquid soap composed mainly of plant oil did not cause irritation of the middle ear mucosa, and was thought to be a promising solvent with which to rinse away tenacious debris, especially when used in combination with hydrogen peroxide.

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