Conditions for a continuous production of transient microbial products in a two-stage fermentation system

1993 ◽  
Vol 15 (8) ◽  
Author(s):  
R. Cubarsi ◽  
A. Villaverde
Keyword(s):  
Continuous Production ◽  
Two Stage ◽  
Fermentation System ◽  
Microbial Products

scholarly journals Continuous production of biohythane from hydrothermal liquefied cornstalk biomass via two-stage high-rate anaerobic reactors

2016 ◽  
Vol 9 (1) ◽  
Author(s):  
Bu-Chun Si ◽  
Jia-Ming Li ◽  
Zhang-Bing Zhu ◽  
Yuan-Hui Zhang ◽  
Jian-Wen Lu ◽  
...  
Keyword(s):  
Continuous Production ◽  
High Rate ◽  
Two Stage ◽  
Anaerobic Reactors

scholarly journals A Two-Stage Continuous Fermentation System for Conversion of Syngas into Ethanol

Energies ◽  
2013 ◽  
Vol 6 (8) ◽  
pp. 3987-4000 ◽  
Author(s):  
Hanno Richter ◽  
Michael Martin ◽  
Largus Angenent
Keyword(s):  
Continuous Fermentation ◽  
Two Stage ◽  
Fermentation System

Enhanced bio-energy recovery in a two-stage hydrogen/methane fermentation process

2009 ◽  
Vol 59 (11) ◽  
pp. 2137-2143 ◽  
Author(s):  
M. J. Lee ◽  
J. H. Song ◽  
S. J. Hwang
Keyword(s):  
Hydrogen Production ◽  
Fermentation Process ◽  
Continuous Production ◽  
Strong Acid ◽  
Treatment Method ◽  
Engineering System ◽  
Production Rates ◽  
Two Stage ◽  
Methane Fermentation ◽  
Pre Treatment

A two-stage hydrogen/methane fermentation process has emerged as a feasible engineering system to recover bio-energy from wastewater. Hydrogen-producing bacteria (HPB) generate hydrogen from readily available carbohydrates, and organic acids produced during the hydrogen fermentation step can be degraded to generate methane in the following step. Three strong acids, HCl, H2SO4, and HNO3, were tested to determine the appropriate pre-treatment method for enhanced hydrogen production. The hydrogen production rates of 230, 290, and 20 L/kg-glucose/day was observed for the sludge treated with HCl, H2SO4, and HNO3, respectively, indicating that the acid pre-treatment using either HCl or H2SO4 resulted in a significant increase in hydrogen production. The fluorescent in situ hybridization method indicated that the acid pre-treatment selectively enriched HPB including Clostridium sp. of cluster I from inoculum sludge. After hydrogen fermentation was terminated, the sludge was introduced to a methane fermentation reactor. This experiment showed methane production rates of 100, 30, and 13 L/kg-glucose/day for the sludge pre-treated with HCl, H2SO4, and HNO3, respectively, implying that both sulfate and nitrate inhibited the activity of methane-producing bacteria. Consequently, the acid pre-treatment might be a feasible option to enhance biogas recovery in the two-stage fermentation process, and HCl was selected as the optimal strong acid for the enrichment of HPB and the continuous production of methane.

Continuous hydrogen and methane production in a two-stage cheese whey fermentation system

2011 ◽  
Vol 64 (2) ◽  
pp. 367-374 ◽  
Author(s):  
C. B. Cota-Navarro ◽  
J. Carrillo-Reyes ◽  
G. Davila-Vazquez ◽  
F. Alatriste-Mondragón ◽  
E. Razo-Flores
Keyword(s):  
Methane Production ◽  
Cheese Whey ◽  
Continuous Production ◽  
Uasb Reactor ◽  
Organic Loading Rate ◽  
Stable Operation ◽  
Operational Parameters ◽  
Two Stage ◽  
Production Of Hydrogen ◽  
Stage Process

The feasibility of integrating biological hydrogen and methane production in a two-stage process using mixed cultures and cheese whey powder (CWP) as substrate was studied. The effect of operational parameters such as hydraulic retention time (HRT) and organic loading rate (OLR) on the volumetric hydrogen (VHPR) and methane (VMPR) production rates was assessed. The highest VHPR was 28 L H2/L/d, obtained during stable operation in a CSTR at HRT and OLR of 6 h and 142 g lactose/L/d, respectively. Moreover, hydrogen (13 L/L/d) was produced even at HRT as low as 3.5 h and OLR of 163 g lactose/L/d, nonetheless, the reactor operation was not stable. Regarding methane production in an UASB reactor, the acidified effluent from the hydrogen-producing bioreactor was efficiently treated obtaining COD removals above 90% at OLR and HRT of 20 g COD/L/d and 6 h, respectively. The two-stage process for continuous production of hydrogen and methane recovered over 70% of the energy present in the substrate. This study demonstrated that hydrogen production can be efficiently coupled to methane production in a two-stage system and that CWP is an adequate substrate for energy production.

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