Histological changes in elastic components of soft palate scars after CO2 and contact Nd:YAG laser incisions in the dog as an experimental model

1996 ◽  
Vol 253 (8) ◽  
Author(s):  
J. Laranne ◽  
S. Matsune ◽  
T. Shima ◽  
M. Ohyama
Keyword(s):  
Experimental Model ◽  
Soft Palate ◽  
Nd:Yag Laser ◽  
Histological Changes ◽  
Contact Nd:Yag Laser

New and safe experimental model of radiation-induced neurovascular histological changes for microsurgical research

2016 ◽  
Vol 51 (2) ◽  
pp. 124-137
Author(s):  
Sergi Barrera-Ochoa ◽  
Irene Gallardo-Calero ◽  
Andrea Sallent ◽  
Alba López-Fernández ◽  
Ramona Vergés ◽  
...  
Keyword(s):  
Side Effects ◽  
Experimental Model ◽  
Experimental Studies ◽  
Neurovascular Bundle ◽  
Control Group ◽  
Sprague Dawley ◽  
Histological Changes ◽  
Group I ◽  
Radiation Induced

The aim is to create a new and safe experimental model of radiation-induced neurovascular histological changes with reduced morbidity and mortality for use with experimental microsurgical techniques. Seventy-two Sprague–Dawley rats (250–300 g) were divided as follows: Group I: control group, 24 rats clinically evaluated during six weeks; Group II: evaluation of acute side-effects (two-week follow-up period), 24 irradiated (20 Gy) rats; and Group III: evaluation of subacute side-effects (six-week follow-up period), 24 irradiated (20 Gy) rats. Variables included clinical assessments, weight, vascular permeability (arterial and venous), mortality and histological studies. No significant differences were observed between groups with respect to the variables studied. Significant differences were observed between groups I vs II–III regarding survival rates and histological changes to arteries, veins and nerves. Rat body weights showed progressive increases in all groups, and the mortality rate of the present model is 10.4% compared with 30–40% in the previous models. In conclusion, the designed model induces selective changes by radiotherapy in the neurovascular bundle without histological changes affecting the surrounding tissues. This model allows therapeutic experimental studies to be conducted, including the viability of microvascular and microneural sutures post radiotherapy in the cervical neurovascular bundle.

scholarly journals A contribution to the study of acute schistosomiasis (an experimental trial)

1987 ◽  
Vol 82 (3) ◽  
pp. 311-317 ◽  
Author(s):  
Zilton A. Andrade ◽  
Theomira Mauadie de Azevedo
Keyword(s):  
Schistosoma Mansoni ◽  
Experimental Model ◽  
Murine Model ◽  
Single Infection ◽  
Coagulative Necrosis ◽  
Histological Changes ◽  
Experimental Trial ◽  
Repeated Infections ◽  
Major Factors ◽  
Inflammatory Changes

In an attempt to establish an experimental model of acute schistosomiasis, sequential histological changes were investigated in the skin, lung, liver and spleen of mice infected with 30 or 100 cercariae of Schistosoma mansoni according to four sets of experiments: single infection, repeated infections, unisexual infection and infection in mice born from infected mothers. Animals were killed every other day from exposure up to 50 days after infection. Only mild, isolated, focal inflammatory changes were found before the appearance of mature eggs in the liver, even when repeated infections were made. Severe changes of reactive hepatitis and splenitis appeared suddenly when the first mature eggs were deposited, around the 37th to 42nd day after infection. The mature eggs induced lytic and coagulative necrosis of hepatocytes around them which was soon followed by dense infiltration of eosinophils. So, mature egg-induced lesions appeared as the major factors in the pathogenesis of acute schistosomiasis in mice. Mice born from infected mothers were apparently able to rapidly modulate the egg-lesions, forming early fibrotic granulomas. The murine model of acute schistosomiasis appeared adequate for the study of pathology and pathogenesis of acute schistosomiasis.

Subglottic Plasmacytoma: The Use of Jet Ventilation and Contact Nd:YAG Laser for Tissue Diagnosis

1993 ◽  
Vol 11 (3) ◽  
pp. 131-134 ◽  
Author(s):  
ROSE C. RABINOV ◽  
DAN J. CASTRO ◽  
THOMAS C. CALCATERRA ◽  
YAO S. FU ◽  
CORRIE T.M. ANDERSON ◽  
...  
Keyword(s):  
Nd:Yag Laser ◽  
Jet Ventilation ◽  
Tissue Diagnosis ◽  
Contact Nd:Yag Laser

External drainage of subretinal fluid with a contact Nd:YAG laser

1987 ◽  
Vol 11 (2) ◽  
pp. 77-78 ◽  
Author(s):  
Gholam A. Peyman ◽  
Howard Charles ◽  
Magdy E. Tawakol ◽  
Jay Federman ◽  
Fumitaka Ando
Keyword(s):  
Nd:Yag Laser ◽  
Subretinal Fluid ◽  
External Drainage ◽  
Contact Nd:Yag Laser

Effects of electrocautery, CO2 laser, and contact Nd:YAG laser scalpel on the healing of intestinal incision

1987 ◽  
Vol 7 (6) ◽  
pp. 507-511 ◽  
Author(s):  
Pauli Puolakkainen ◽  
Kim Brackett ◽  
M. Y. Sankar ◽  
Steven Joffe ◽  
Tom Schröder
Keyword(s):  
Co2 Laser ◽  
Nd:Yag Laser ◽  
Laser Scalpel ◽  
Contact Nd:Yag Laser

Transendoscopic Nd:YAG Laser Surgery for Treatment of Epiglottal Entrapment and Dorsal Displacement of the Soft Palate in the Horse

1990 ◽  
Vol 19 (5) ◽  
pp. 356-363 ◽  
Author(s):  
LLOYD P. TATE ◽  
CRAIG L. SWEENEY ◽  
KARL F. BOWMAN ◽  
HY C. NEWMAN ◽  
WENDY M. DUCKETT
Keyword(s):  
Soft Palate ◽  
Nd:Yag Laser ◽  
Laser Surgery ◽  
Nd:Yag Laser Surgery

An Experimental Model of Eosinophilic Chronic Rhinosinusitis Induced by Bacterial Toxins in Rabbits

2019 ◽  
Vol 33 (6) ◽  
pp. 737-750 ◽  
Author(s):  
Andréa A. Braga ◽  
Fabiana C. P. Valera ◽  
Francesca M. Faria ◽  
Maria Rossato ◽  
Adriana A. B. Murashima ◽  
...  
Keyword(s):  
Experimental Model ◽  
Chronic Rhinosinusitis ◽  
Prolonged Exposure ◽  
Inflammatory Cells ◽  
Lipoteichoic Acid ◽  
Bacterial Toxins ◽  
Bacterial Toxin ◽  
Indwelling Catheter ◽  
Histological Changes ◽  
Mucosal Thickening

Background The pathophysiology of chronic rhinosinusitis (CRS) is still not well known due to the multifactorial etiologies involved. Bacteria play a role in the pathogenesis of CRS by various means, including biofilm adhesion, intracellular persistence, or inducing inflammation secondary to toxins. Endotoxins and exotoxins, especially Staphylococcus aureus superantigens, can produce significant immune responses in the host and are implicated in patients with CRS. The majority of animal models described for CRS revalidates the pathophysiology of acute sinusitis, ostium occlusion, or foreign body associated infection. Objectives To evaluate an experimental model of eosinophilic CRS using prolonged exposure to bacterial toxins. The histological changes in rabbits exposed to S. aureus enterotoxin B (SEB), lipopolysaccharide (LPS), or lipoteichoic acid (LTA) were compared. Methods After induction with ovalbumin (OVA) sensitization with subcutaneous injection for 2 weeks, rabbits underwent surgery to insert an indwelling catheter into the maxillary sinus. The sinus was irrigated with OVA 3 times weekly for 2 weeks, followed by sinus irrigation with bacterial toxin (SEB: 1 µg/mL, LPS: 100 ng/mL, or LTA: 100 ng/mL) 3 times weekly for 4 weeks. The histological changes in the treated sinus were compared with control rabbits. Results Sinuses exposed to bacterial toxins (SEB, LPS, and LTA) produced significant mucosal thickening with infiltration of inflammatory cells, notably eosinophils. SEB was the only toxin that promoted a mixed pattern of inflammation, including eosinophilic and neutrophilic infiltration. Conclusion Our experimental model of eosinophilic CRS in rabbits produced significant mucosal thickening and inflammation in the sinuses exposed to bacterial toxins, with histological changes analogous to what is observed in patients with CRS with nasal polyps. This model may serve as a basis for future investigation of the pathogenesis of eosinophilic CRS in relation to bacterial toxins or as a model for testing new therapeutic modalities for this disease.

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