In vitro expression of Lac-PTS and togatose 1,6-biphosphate aldolase genes from Lactococcus lactis subsp. cremoris plasmid pDI-21

1992 ◽  
Vol 37 (2) ◽  
Author(s):  
P.L. Yu ◽  
R.A. Hodge ◽  
X.P. Li
Keyword(s):  
Lactococcus Lactis ◽  
In Vitro Expression ◽  
Lactococcus Lactis Subsp

scholarly journals Complete Genome Sequence of Lactococcus lactis subsp. lactis Strain 14B4, Which Inhibits the Growth of Salmonella enterica Serotype Poona In Vitro

2018 ◽  
Vol 7 (19) ◽  
Author(s):  
Thao D. Tran ◽  
Steven Huynh ◽  
Craig T. Parker ◽  
Ruyang Han ◽  
Robert Hnasko ◽  
...  
Keyword(s):  
Lactococcus Lactis ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Salmonella Enterica ◽  
Complete Genome ◽  
Northern California ◽  
Content Type ◽  
Lactococcus Lactis Subsp ◽  
Lactis Strain

We present here the complete genome sequence of Lactococcus lactis strain 14B4, isolated from almond drupes in northern California. This strain was observed to inhibit the growth of Salmonella enterica serotype Poona strain RM3363 in vitro.

The antibacterial activity expressed in vitro and in vivo by Lactococcus lactis subsp. lactis CNRZ 1427 against Salmonella sp.

2015 ◽  
Vol 4 (5) ◽  
pp. 240-246
Author(s):  
Menad Najett ◽  
Chougrani Fadelaa ◽  
Moghtet Snoussi ◽  
Cheriguene Abderrahim
Keyword(s):  
Antibacterial Activity ◽  
Lactococcus Lactis ◽  
Bile Salts ◽  
Therapeutic Agent ◽  
Lactococcus Lactis Subsp ◽  
Beneficial Effects ◽  
Gastrointestinal Conditions ◽  
Therapeutic Possibilities

  The present study focused on the beneficial effects of Lactococcus lactis subsp lactis CNRZ 1427 with possible use as a therapeutic agent against Sal-monella sp.; also we have proposed different therapeutic possibilities of our situation against a pathogen Salmonella sp. We have conducted two tests In Vitro and In Vivo; where it is noted that treatment in the presence of this lac-tic strain is effective since it causes a remarkable decrease of the pathogen agent. At the end, the effectiveness of this lactic strain was confirmed by testing for resistance to gastrointestinal conditions (pH, bile salts and diges-tive enzymes).

scholarly journals In vitro assessment of hypocholesterolemic activity of Lactococcus lactis subsp. lactis

2019 ◽  
Vol 43 (1) ◽  
Author(s):  
Mohamed G. Shehata ◽  
Malak A. El-Sahn ◽  
Sobhy A. El Sohaimy ◽  
Mohamed M. Youssef
Keyword(s):  
Lactococcus Lactis ◽  
Lactococcus Lactis Subsp ◽  
In Vitro Assessment

Lactococcus lactis subsp. cremoris strain JFR1 attenuates Salmonella adhesion to human intestinal cells in vitro

2016 ◽  
Vol 90 ◽  
pp. 147-153 ◽  
Author(s):  
Justina Su Zhang ◽  
Anilda Guri ◽  
Milena Corredig ◽  
Rocio Morales-Rayas ◽  
Ashraf Hassan ◽  
...  
Keyword(s):  
Lactococcus Lactis ◽  
Intestinal Cells ◽  
Lactococcus Lactis Subsp ◽  
Human Intestinal Cells

Survival of lactococci during passage through mouse digestive tract

2003 ◽  
Vol 49 (11) ◽  
pp. 707-711 ◽  
Author(s):  
Hiromi Kimoto ◽  
Masaru Nomura ◽  
Miho Kobayashi ◽  
Koko Mizumachi ◽  
Takashi Okamoto
Keyword(s):  
Survival Rate ◽  
Lactococcus Lactis ◽  
Animal Health ◽  
In Vitro Test ◽  
Lactococcus Lactis Subsp ◽  
Mouse Intestine ◽  
Vitro Test ◽  
The Subject

One of the important properties of probiotics is the ability to survive in the intestine. There have been few studies on the probiotic property of lactococci, since they are formally not considered to be natural inhabitants of the intestine. To evaluate lactococci as probiotic bacteria, we investigated their ability to survive during gastric transit by in vitro and in vivo tests. When exposed to an in vitro simulated gastrointestinal environment, such as low pH and bile, only Lactococcus lactis subsp. lactis bv. diacetylactis N7 showed a moderate survival rate among the four strains tested. The tested strains were orally administered to mice, and intestinal passage of the ingested strains was monitored by two methods: antibiotics and PCR. Viable cells of strain N7 were recovered from feces within 24–48 h after administration but not at 72 h. Lactococcus lactis subsp. cremoris ATCC 19257, which had a poor survival rate in vitro test, was also detected at 12 h but not at 24 h. These results indicate that lactococci can reach the mouse intestine alive, but not colonize it. If administered daily, viable strain N7 may exist continuously in the intestine. The effect of strain N7 on intestinal microbial balance and on animal health will be the subject of a further study.Key words: Lactococcus, survival, gastrointestinal tract, probiotics.

scholarly journals Evaluation of Some Factors with Significant Influence on Functionality and Viability of Probiotic Strains LA-5 ® and L. Casei 431 ® in Multiple Starter Cultures in Mesophilic Fermented Milk

2012 ◽  
Vol 69 (2) ◽  
Author(s):  
Daniela PARASCHIV ◽  
Aida VASILE
Keyword(s):  
Lactococcus Lactis ◽  
Fermented Milk ◽  
Starter Cultures ◽  
Milk Product ◽  
Lactococcus Lactis Subsp ◽  
Commercial Code ◽  
Long Time ◽  
Probiotic Strains

Abstract.  For a very long time, probiotics have been selected on the basis of their suitability to food’s environment and technological procedures, as well as survival ability in vitro and also in vivo. These criteria are still used. The aim of this study was to select, using the Plakett-Burman design of experiments model, of three significant factors, which influence the multiplication ability and viability of probiotic strains Lactobacillus acidophilus (commercial code LA-5®) and Lactobacillus casei subsp. paracasei (commercial code L. casei 431®) in fermented mesophilic milk product (commercial named Sana) obtained by fermentation with a multiple culture with mesophilic lactic starter Flora Danica Chr. Hansen commercial starters (Lactococcus lactis subsp. cremoris, Lactococcus lactis subsp. lactis, Lactococcus lactis subsp. diacetylactis and Leuconostoc mesenteroides subsp. cremoris). By statistical analysis the most important factors (independent variables)  with influence on the probiotic bacteria were selected for the optimized. These are the temperature of fermentation; the ratio between probiotic strains and Flora Danica and the addition of prebiotic Fagopyrum esculentum flour. In the future experiments the optimum values of these parameters will be optimized by using response surface methodology, in order to establish the conditions in which can be obtain the mesophilic fermented milk products with probiotic action in vivo. 

In vitro expression of the restriction endonucleases LlaMI and ScrFI isolated from Lactococcus lactis M19 and UC503

2006 ◽  
Vol 121 (2) ◽  
pp. 144-153 ◽  
Author(s):  
George Szatmari ◽  
Ngiep Man Hua ◽  
Dimitri Vzdornov ◽  
François Daigle ◽  
Wanda Smoragiewicz ◽  
...  
Keyword(s):  
Lactococcus Lactis ◽  
Restriction Endonucleases ◽  
In Vitro Expression

scholarly journals Antimicrobial activity of the Nisin Z producer Lactococcus lactis subsp. lactis Lc08 against Listeria monocytogenes in skim milk

2013 ◽  
Vol 65 (5) ◽  
pp. 1554-1560 ◽  
Author(s):  
L.M. Perin ◽  
R.O. Miranda ◽  
A.C. Camargo ◽  
M. Colombo ◽  
A.F. Carvalho ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Listeria Monocytogenes ◽  
Lactococcus Lactis ◽  
Food Systems ◽  
Skim Milk ◽  
In Vitro Tests ◽  
Lactococcus Lactis Subsp ◽  
Ph Values ◽  
Nisin Z

The presented study aimed to verify the effect of different pH values, enzyme solutions and heat treatments on the antimicrobial activity of the bacteriocinogenic strain Lactococcus lactis subsp. lactis Lc08 and to test their antimicrobial activity against Listeria monocytogenes in reconstituted skim milk at refrigeration temperatures. This strain was previously described as a nisin Z producer and capable of inhibiting L. monocytogenes growth in in vitro tests. The antimicrobial activity of the bacteriocin cell-free supernatant of Lc08 was sensitive to enzyme treatments (except papain). The pH values and heating (65ºC for 30min, 75ºC for 15s) had no apparent effect on the antimicrobial activity of the bacteriocin produced by Lc08. Only treatment at autoclave conditions result in loss of their antimicrobial activity. Lc08 presented antimicrobial activity against L. monocytogenes in the milk system after 12h at 25ºC. No effect was found at 7ºC. The results show the application viability of the Lc08 in food systems as a biopreservative against L. monocytogenes.

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