In vitro activity of 4? -iodo-4? -deoxydoxorubicin on human colo-rectal cancer as measured by a short-term antimetabolic assay

1990 ◽  
Vol 8 (2) ◽  
Author(s):  
Raffaella Villa ◽  
Nadia Zaffaroni ◽  
Fernando Giuliani ◽  
Giovanni Colella ◽  
Ornella Sanfilippo ◽  
...  
Keyword(s):  
Rectal Cancer ◽  
Vitro Activity ◽  
In Vitro Activity ◽  
Short Term

In Vitro Activity of Alkylating Agents on Human Tumors as Measured by a Short-term Antimetabolic Assay

1985 ◽  
Vol 71 (6) ◽  
pp. 555-561 ◽  
Author(s):  
Nadia Zafferoni ◽  
Rosella Silvestrini ◽  
Ornella Sanfilippo ◽  
Maria Grazia Daidone ◽  
Giampietro Gasparini
Keyword(s):  
Alkylating Agents ◽  
Human Tumors ◽  
Vitro Activity ◽  
In Vitro Activity ◽  
Short Term

Photosynthesis, carbon partitioning and metabolite content during drought stress in peach seedlings

1998 ◽  
Vol 25 (2) ◽  
pp. 197 ◽  
Author(s):  
A. J. Escobar-Gutiérrez ◽  
B. Zipperlin ◽  
F. Carbonne ◽  
A. Moing ◽  
J.P. Gaudillére
Keyword(s):  
Drought Stress ◽  
Osmotic Adjustment ◽  
Osmotic Potential ◽  
Vitro Activity ◽  
In Vitro Activity ◽  
Short Term ◽  
Phloem Sap ◽  
Mature Leaves ◽  
Key Enzyme

Photosynthesis, metabolic carbon partitioning and the contribution of sorbitol to the osmotic potential of mature peach (Prunus persica (L.) Batsch) leaves and phloem sap were examined in plants undergoing two levels of a short term drought stress. The relationship between osmotic potential at full turgor and water potential showed that neither mild nor severe drought stress induced a significant active osmotic adjustment in mature leaves. The osmotic potential of leaves at full turgor was -1.9 MPa; sorbitol was the major organic component (20%). Leaf sucrose and starch contents were significantly reduced by drought. The partitioning of newly-fixed carbon was also affected by stress. These changes appeared to originate from the inhibition of photosynthesis induced by drought stress. At low photosynthetic rates, the turnover of organic ions was low, and sorbitol synthesis was favoured over that of sucrose. Water stress did not affect the in vitro activity of sucrose phosphate synthase (EC 2.4.1.14), the key enzyme in sucrose synthesis. The in vitro activity of aldose-6-phosphate reductase (EC 1.1.1.200), the key enzyme in sorbitol synthesis, tended to increase linearly in response to drought stress. It is concluded that, contrary to some other polyol-synthesising species, peach did not seem to benefit from sorbitol synthesis during short term drought stress for active osmotic adjustment in mature leaves. However, in phloem sap, increases in sucrose and especially sorbitol concentration were observed in stressed plants.

scholarly journals In Vitro Activity of Tebipenem, a New Oral Carbapenem Antibiotic, against Penicillin-Nonsusceptible Streptococcus pneumoniae

2005 ◽  
Vol 49 (3) ◽  
pp. 889-894 ◽  
Author(s):  
Reiko Kobayashi ◽  
Mami Konomi ◽  
Keiko Hasegawa ◽  
Miyuki Morozumi ◽  
Keisuke Sunakawa ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Respiratory Tract Infections ◽  
Multidrug Resistant ◽  
Vitro Activity ◽  
In Vitro Activity ◽  
Short Term ◽  
Short Term Exposure ◽  
Tract Infections ◽  
Carbapenem Antibiotic

ABSTRACT The in vitro activity of tebipenem (TBM), a new oral carbapenem antibiotic, against Streptococcus pneumoniae clinical isolates (n = 202) was compared with those of 15 reference agents. The isolates were classified into five genotypic classes after PCR identification of abnormal pbp1a, pbp2x, and pbp2b genes: (i) penicillin-susceptible S. pneumoniae (PSSP) isolates with no abnormal pbp genes (n = 34; 16.8%), (ii) genotypic penicillin-intermediate S. pneumoniae (gPISP) isolates with only an abnormal pbp2x gene [gPISP (2x)] (n = 48; 23.8%), (iii) gPISP isolates with abnormal pbp1a and pbp2x genes (n = 32; 15.8%), (iv) gPISP isolates with abnormal pbp2x and pbp2b genes (n = 16; 7.9%), and (v) genotypic penicillin-resistant S. pneumoniae (gPRSP) isolates with three abnormal pbp genes (n = 72; 35.6%). The majority of the strains tested had mefA (n = 59; 29.2%) or ermB (n = 91; 45%) gene-mediating macrolide resistance. For these isolates the MIC at which 90% of isolates are inhibited was significantly lower for TBM than for the reference oral antibiotics, as follows: 0.002 μg/ml for PSSP, 0.004 μg/ml for gPISP (2x), 0.016 μg/ml for gPISP (isolates with abnormal pbp1a and pbp2x genes and isolates with abnormal pbp2x and pbp2b genes), and 0.063 μg/ml for gPRSP. In addition, TBM showed excellent bactericidal activity against gPRSP isolates, which exhibited a 3-log10 decrease within 2 h when they were incubated with a concentration greater than or equal to the MIC. Inhibition of cell wall synthesis toward the long axis and subsequent cell lysis were observed by scanning electron microscopy after a short-term exposure to TBM, unlike the effects seen with cephalosporins. These data suggest that TBM has potent activity against multidrug-resistant S. pneumoniae, the causative pathogen of community-acquired respiratory tract infections.

In Vitro Activity of Phytocannabinoids from High Potency Cannabis sativa

Planta Medica ◽  
2012 ◽  
Vol 78 (05) ◽  
Author(s):  
A Husni ◽  
S Ross ◽  
O Dale ◽  
C Gemelli ◽  
G Ma ◽  
...  
Keyword(s):  
Cannabis Sativa ◽  
Vitro Activity ◽  
In Vitro Activity ◽  
High Potency
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