Rapid cloning of any rearranged mouse immunoglobulin variable genes

1996 ◽  
Vol 43 (3) ◽  
Author(s):  
AnupamK. Dattamajumdar ◽  
DavidP. Jacobson ◽  
LeroyE. Hood ◽  
GamalE. Osman
Keyword(s):  
Mouse Immunoglobulin ◽  
Immunoglobulin Variable Genes ◽  
Variable Genes

DNA polymerase η is an A-T mutator in somatic hypermutation of immunoglobulin variable genes

10.1038/88740 ◽  
2001 ◽  
Vol 2 (6) ◽  
pp. 537-541 ◽  
Author(s):  
Xianmin Zeng ◽  
David B. Winter ◽  
Cynthia Kasmer ◽  
Kenneth H. Kraemer ◽  
Alan R. Lehmann ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Somatic Hypermutation ◽  
Immunoglobulin Variable Genes ◽  
Variable Genes

Somatic mutations of immunoglobulin variable genes are restricted to the rearranged V gene

Science ◽  
1983 ◽  
Vol 220 (4602) ◽  
pp. 1179-1181 ◽  
Author(s):  
J Gorski ◽  
P Rollini ◽  
B Mach
Keyword(s):  
Somatic Mutations ◽  
Immunoglobulin Variable Genes ◽  
V Gene ◽  
Variable Genes

scholarly journals Patterns of Somatic Mutations in Immunoglobulin Variable Genes

Genetics ◽  
1987 ◽  
Vol 115 (1) ◽  
pp. 169-176
Author(s):  
G Brian Golding ◽  
Patricia J Gearhart ◽  
Barry W Glickman
Keyword(s):  
Somatic Mutation ◽  
Dna Sequences ◽  
Somatic Mutations ◽  
Nucleotide Substitutions ◽  
Immunoglobulin Variable Genes ◽  
Variable Gene ◽  
Meiotic Mutations ◽  
Mutational Processes ◽  
Palindromic Sequences ◽  
Variable Genes

ABSTRACT The mechanism responsible for somatic mutation in the variable genes of antibodies is unknown and may differ from previously described mechanisms that produce mutation in DNA. We have analyzed 421 somatic mutations from the rearranged immunoglobulin variable genes of mice to determine (1) if the nucleotide substitutions differ from those generated during meiosis and (2) if the presence of nearby direct and inverted repeated sequences could template mutations around the variable gene. The results reveal a difference in the pattern of substitutions obtained from somatic mutations vs. meiotic mutations. An increased frequency of T:A to C:G transitions and a decreased frequency of mutations involving a G in the somatic mutants compared to the meiotic mutants is indicated. This suggests that the mutational processes responsible for somatic mutation in antibody genes differs from that responsible for mutation during meiosis. An analysis of the local DNA sequences revealed many direct repeats and palindromic sequences that were capable of templating some of the known mutations. Although additional factors may be involved in targeting mutations to the variable gene, mistemplating by nearby repeats may provide a mechanism for the enhancement of somatic mutation.

Recombination signature of germline immunoglobulin variable genes

1998 ◽  
Vol 76 (2) ◽  
pp. 179-185 ◽  
Author(s):  
Georg F Weiller ◽  
Harald S Rothenfluh ◽  
Paula Zylstra ◽  
Lynn M Gay ◽  
Holger Averdunk ◽  
...  
Keyword(s):  
Immunoglobulin Variable Genes ◽  
Variable Genes
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