3D 13C/1H NMR-based assignments for side-chain resonances of Lactobacillus casei dihydrofolate reductase. Evidence for similarities between the solution and crystal structures of the enzyme

1993 ◽  
Vol 3 (5) ◽  
pp. 535-546 ◽  
Author(s):  
A. Soteriou ◽  
M. D. Carr ◽  
T. A. Frenkiel ◽  
J. E. McCormick ◽  
C. J. Bauer ◽  
...  
Keyword(s):  
Crystal Structures ◽  
1H Nmr ◽  
Dihydrofolate Reductase ◽  
Lactobacillus Casei ◽  
Side Chain

The 1H-NMR assignments of the aromatic resonances in complexes of Lactobacillus casei dihydrofolate reductase and the origins of their chemical shifts

1990 ◽  
Vol 191 (3) ◽  
pp. 659-668 ◽  
Author(s):  
Berry BIRDSALL ◽  
John R. P. ARNOLD ◽  
Jesus JIMENEZ-BARBERO ◽  
Thomas A. FRENKIEL ◽  
Christopher J. BAUER ◽  
...  
Keyword(s):  
1H Nmr ◽  
Dihydrofolate Reductase ◽  
Lactobacillus Casei ◽  
Nmr Assignments ◽  
Chemical Shifts

1H-NMR - und ESR -Messungen zur Struktur der Dehydroascorbinsäure und des Semidehydroascorbatradikals / 1H-NMR- and ESR-Investigations on the Structures of Dehydroascorbic Acid and the Semidehydroascorbate Radical

1982 ◽  
Vol 37 (1-2) ◽  
pp. 129-131 ◽  
Author(s):  
Helmut Sapper ◽  
Anita Pleyer-Weber ◽  
Wolfgang Lohmann
Keyword(s):  
Electron Spin Resonance ◽  
1H Nmr ◽  
Electron Spin ◽  
Dehydroascorbic Acid ◽  
Side Chain ◽  
Bicyclic Structure ◽  
Spin Resonance

By comparing proton and electron spin resonance spec­tra of twofold and single oxidized ascorbate and acetylated derivatives therefrom it can be concluded that semidehy- droascorbate as well as dehydroascorbate are of bicyclic structure due to the formation of a furanose ring of their side chain.

Crystal structures of recombinant human dihydrofolate reductase complexed with folate and 5-deazafolate

Biochemistry ◽  
1990 ◽  
Vol 29 (40) ◽  
pp. 9467-9479 ◽  
Author(s):  
Jay F. Davies ◽  
Tavner J. Delcamp ◽  
Neal J. Prendergast ◽  
Victor A. Ashford ◽  
James H. Freisheim ◽  
...  
Keyword(s):  
Crystal Structures ◽  
Dihydrofolate Reductase ◽  
Human Dihydrofolate Reductase

1H NMR, EPR, UV−Vis, and Electrochemical Studies of Imidazole Complexes of Ru(III). Crystal Structures ofcis-[(Im)2(NH3)4RuIII]Br3and [(1MeIm)6RuII]Cl2·2H2O

1996 ◽  
Vol 35 (17) ◽  
pp. 4896-4903 ◽  
Author(s):  
M. J. Clarke ◽  
V. M. Bailey ◽  
P. E. Doan ◽  
C. D. Hiller ◽  
K. J. LaChance-Galang ◽  
...  
Keyword(s):  
Crystal Structures ◽  
1H Nmr ◽  
Electrochemical Studies ◽  
Imidazole Complexes

Structural Studies on N-(2,4,6-Trimethylphenyl)-methyl/ chloro-acetamides, 2,4,6-(CH3)3C6H2NH-CO-CH3–yXy (X = CH3 or Cl and y = 0, 1, 2)

2006 ◽  
Vol 61 (10-11) ◽  
pp. 588-594 ◽  
Author(s):  
Basavalinganadoddy Thimme Gowda ◽  
Jozef Kožíšek ◽  
Hartmut Fuess
Keyword(s):  
Crystal Structure ◽  
Crystal Structures ◽  
Structural Data ◽  
The Other ◽  
Side Chain ◽  
Structural Studies ◽  
Asymmetric Unit ◽  
Lattice Constants ◽  
Peptide Linkage ◽  
The Mean

TMPAThe effect of substitutions in the ring and in the side chain on the crystal structure of N- (2,4,6-trimethylphenyl)-methyl/chloro-acetamides of the configuration 2,4,6-(CH3)3C6H2NH-COCH3− yXy (X = CH3 or Cl and y = 0,1, 2) has been studied by determining the crystal structures of N-(2,4,6-trimethylphenyl)-acetamide, 2,4,6-(CH3)3C6H2NH-CO-CH3 (); N-(2,4,6- trimethylphenyl)-2-methylacetamide, 2,4,6-(CH3)3C6H2NH-CO-CH2-CH3 (TMPMA); N-(2,4,6- trimethylphenyl)-2,2-dimethylacetamide, 2,4,6-(CH3)3C6H2NH-CO-CH(CH3)2 (TMPDMA) and N-(2,4,6-trimethylphenyl)-2,2-dichloroacetamide, 2,4,6-(CH3)3C6H2NH-CO-CHCl2 (TMPDCA). The crystallographic system, space group, formula units and lattice constants in Å are: TMPA: monoclinic, Pn, Z = 2, a = 8.142(3), b = 8.469(3), c = 8.223(3), β = 113.61(2)◦; TMPMA: monoclinic, P21/n, Z = 8, a = 9.103(1), b = 15.812(2), c = 16.4787(19), α = 89.974(10)◦, β = 96.951(10)◦, γ =89.967(10)◦; TMPDMA: monoclinic, P21/c, Z = 4, a =4.757(1), b= 24.644(4), c =10.785(2), β = 99.647(17)◦; TMPDCA: triclinic, P¯1, Z = 2, a = 4.652(1), b = 11.006(1), c = 12.369(1), α = 82.521(7)◦, β = 83.09(1)◦, γ = 79.84(1)◦. The results are analyzed along with the structural data of N-phenylacetamide, C6H5NH-CO-CH3; N-(2,4,6-trimethylphenyl)-2-chloroacetamide, 2,4,6-(CH3)3C6H2NH-CO-CH2Cl; N-(2,4,6-trichlorophenyl)-acetamide, 2,4,6-Cl3C6H2NH-COCH3; N-(2,4,6-trichlorophenyl)-2-chloroacetamide, 2,4,6-Cl3C6H2NH-CO-CH2Cl; N-(2,4,6-trichlorophenyl)- 2,2-dichloroacetamide, 2,4,6-Cl3C6H2NH-CO-CHCl2 and N-(2,4,6-trichlorophenyl)- 2,2,2-trichloroacetamide, 2,4,6-Cl3C6H2NH-CO-CCl3. TMPA, TMPMA and TMPDCA have one molecule each in their asymmetric units, while TMPDMA has two molecules in its asymmetric unit. Changes in the mean ring distances are smaller on substitution as the effect has to be transmitted through the peptide linkage. The comparison of the other bond parameters reveal that there are significant changes in them on substitution.

scholarly journals A 1H n.m.r. study of the role of the glutamate moiety in the binding of methotrexate to Lactobacillus casei dihydrofolate reductase

1984 ◽  
Vol 81 (2) ◽  
pp. 309-315 ◽  
Author(s):  
David J. Antonjuk ◽  
Berry Birdsall ◽  
H.T. Andrew Cheung ◽  
G. Marius Clore ◽  
James Feeney ◽  
...  
Keyword(s):  
Dihydrofolate Reductase ◽  
Lactobacillus Casei

Dihydrofolate reductase from amethopterin-resistant Lactobacillus casei

Biochemistry ◽  
1972 ◽  
Vol 11 (6) ◽  
pp. 1018-1023 ◽  
Author(s):  
L. E. Gundersen ◽  
R. B. Dunlap ◽  
N. G. L. Harding ◽  
J. H. Freisheim ◽  
F. Otting ◽  
...  
Keyword(s):  
Dihydrofolate Reductase ◽  
Lactobacillus Casei
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