Start-up of a thermophilic upflow anaerobic sludge bed (UASB) reactor with mesophilic granular sludge

1992 ◽  
Vol 37 (1) ◽  
pp. 130-135 ◽  
Author(s):  
Jules B. van Lier ◽  
Katja C. F. Grolle ◽  
Alfons J. M. Stams ◽  
Everly Conway de Macario ◽  
Gatze Lettinga
Keyword(s):  
Granular Sludge ◽  
Uasb Reactor ◽  
Anaerobic Sludge ◽  
Start Up

Start up of Thermophilic UASB (Upflow Anaerobic Sludge Blanket) Reactors Using Micro-Carrier and Mesophilic Granular Sludge

1992 ◽  
Vol 26 (3-4) ◽  
pp. 877-886 ◽  
Author(s):  
T. Ohtsuki ◽  
M. Watanabe ◽  
Y. Miyaji
Keyword(s):  
High Performance ◽  
Ph Dependence ◽  
Granular Sludge ◽  
Upflow Anaerobic Sludge Blanket ◽  
Uasb Reactor ◽  
Anaerobic Sludge ◽  
Phase System ◽  
Two Phase ◽  
Start Up ◽  
Initial Seed

Two start-up methods of thermophilic UASB reactor were investigated for fast start-ups; one utilized micro-carrier as an initial support material and the other used intact mesophilic UASB granules as an initial seed. With both methods thermophilic granules having high activity were obtained in less than 3 months, even with acidified wastewater. Maximum sludge load for VFA substrate and sugar substrate were 3.2 and 0.9 kgCOD/kgVSS/day, respectively. Pre-acidogenesis was indispensable for high-performance treatment of sugar-containing substrate. It was proven that with a two-phase system sludge load could be raised to 3.0 kgCOD/kgVSS/day for sugar containing wastewaters. Some features of obtained sludge were examined, including maximum substrate utilizing activities, temperature dependence, pH dependence, and activity deterioration under lower loading conditions.

Removal of COD from Piggery Wastewater Using a Lab-Scale UASB Reactor

2012 ◽  
Vol 534 ◽  
pp. 221-224
Author(s):  
Fei Yan ◽  
Jin Long Zuo ◽  
Tian Lei Qiu ◽  
Xu Ming Wang
Keyword(s):  
Granular Sludge ◽  
Cod Removal ◽  
Upflow Anaerobic Sludge Blanket ◽  
Synthetic Wastewater ◽  
Uasb Reactor ◽  
Anaerobic Sludge ◽  
Volume Loading ◽  
Piggery Wastewater ◽  
Start Up ◽  
Anaerobic Sludge Blanket

It took 55 days to start up a lab-scale upflow anaerobic sludge blanket (UASB) reactor at ambient temperature 27-28 oC by using the synthetic wastewater, and piggery wastewater was used as the influent after the reactor start-up. From day 120 onwards, COD removal efficiency maintained in the range of 85% to 95% with 6.79-9.66 kg COD/ (m3•d) of volume loading, and the effluent COD concentration ranged between 400 mg/L and 600 mg/L. Granular sludge formation was observed in the reactor after 40-day operation, and the sludge diameter reached 2-4 mm in the 120 day-old reactor. The pH changes in the influent had little influence on COD removal from piggery wastewater using the UASB reactor.

High-Rate Anaerobic Treatment of Industrial Wastewaters

1991 ◽  
Vol 24 (1) ◽  
pp. 69-74 ◽  
Author(s):  
J. Rintala
Keyword(s):  
Granular Sludge ◽  
Anaerobic Treatment ◽  
High Rate ◽  
Upflow Anaerobic Sludge Blanket ◽  
Synthetic Wastewater ◽  
Uasb Reactor ◽  
Anaerobic Sludge ◽  
Industrial Wastewaters ◽  
Start Up ◽  
Uasb Reactors

Anaerobic mesophilic treatment of synthetic (a mixture of acetate and methanol) and thermomechanical pulping (TMP) wastewater was studied in laboratory-scale upflow anaerobic sludge blanket (UASB) reactors and filters with emphasis on the process start-up. The reactors were inoculated with nongranular sludge. The start-up of mesophilic and thermophilic processes inoculated with mesophilic granular sludge was investigated in UASB reactors fed with diluted vinasse. The start-up proceeded faster in the filters than in the UASB reactors with TMP and synthetic wastewater. Loading rates of over 15 kgCODm−3d−1 with 50-60 % COD removal efficiencies were achieved in 10 days in the mesophilic and in 50 days in the thermophilic UASB reactor treating vinasse. The results show that high-rate anaerobic treatment can be applied to different types of industrial wastewaters under varying conditions.

Start-up of a thermophilic methanol-fed UASB reactor: change in sludge characteristics

2002 ◽  
Vol 45 (10) ◽  
pp. 145-150 ◽  
Author(s):  
P.L. Paulo ◽  
B. Jiang ◽  
K. Roest ◽  
J.B. van Lier ◽  
G. Lettinga
Keyword(s):  
Granular Sludge ◽  
Community Analysis ◽  
Uasb Reactor ◽  
Anaerobic Sludge ◽  
Sludge Characteristics ◽  
Loading Rates ◽  
Start Up ◽  
Laboratory Scale Reactor ◽  
Methanol Removal ◽  
Scale Reactor

Experiments were performed to study the change in sludge characteristics and sludge granulation during the start-up of a thermophilic methanol-fed upflow anaerobic sludge bed (UASB) reactor. The laboratory scale reactor, was inoculated with thermophilic granular sludge and operated at 55°C over 130 days at organic loading rates (OLR) varying from 2.7 to 47 gCOD.L−1.d−1. Physical characterisation was performed for both the seed and the cultivated sludge. Results demonstrated that a good quality, well settleable granular sludge was cultivated and retained in the reactor, allowing an OLR of 47 gCOD.L−1.d−1 with 93% of methanol removal, where 79% was converted into methane. Using a community analysis of the cultivated consortia, high numbers of rod-shaped hydrogenotrophic methanogens were enumerated. Biomass washout coincided with a high specific gas load, but was not detrimental to the system in the conditions tested.

The effect of extreme temperatures (70–80°C) on the effluent quality and sludge characteristics of UASB reactors

1997 ◽  
Vol 36 (6-7) ◽  
pp. 325-332 ◽  
Author(s):  
Raghida Lepistö ◽  
Jukka Rintala
Keyword(s):  
Volatile Fatty Acids ◽  
Granular Sludge ◽  
Anaerobic Sludge ◽  
Methanogenic Activity ◽  
Sludge Characteristics ◽  
Effluent Quality ◽  
Start Up ◽  
Thermomechanical Pulping ◽  
Increasing Temperature ◽  
Uasb Reactors

The study focused on the effluent quality and sludge characteristics during the start-up and operation of extreme thermophilic (70 to 80°C) upflow anaerobic sludge bed (UASB) reactors, inoculated with mesophilic and thermophilic granular sludge and fed with acetate, volatile fatty acids (VFA), and thermomechanical pulping (TMP) whitewater. Low effluent quality and long start-up periods were observed during the start-up of the 70 to 76°C, VFA-fed UASB reactors inoculated with mesophilic granulae, while better effluent quality and considerably shorter start-up periods were observed when thermophilic (55/70°C) inocula were used. With VFA feed, a significant amount of acetate was removed at 70°C and even at 80°C, while propionate removal was negligible. With TMP whitewater feed, low VFA effluent concentration was obtained at 70°C. The volatile solids (VS) and the VS/total solids (TS) content of the sludge decreased significantly during the first 2–3 months of operation when mesophilic inocula were used. The initial specific methanogenic activity (ISMA) of the extreme thermophilic sludge decreased with increasing temperature and was slightly higher on glucose than on acetate. At 70 to 80°C, various rod-like bacteria were dispersed through the granulae in either individual or in low density micro colonies surrounded with a varying degree of precipitates.

scholarly journals Microbial Diversity Dynamics in a Methanogenic-Sulfidogenic UASB Reactor

2021 ◽  
Vol 18 (3) ◽  
pp. 1305
Author(s):  
E. Fernández-Palacios ◽  
Xudong Zhou ◽  
Mabel Mora ◽  
David Gabriel
Keyword(s):  
Paper Industry ◽  
Chemical Species ◽  
Methanogenic Archaea ◽  
Granular Sludge ◽  
Upflow Anaerobic Sludge Blanket ◽  
Uasb Reactor ◽  
Anaerobic Sludge ◽  
Rrna Gene ◽  
Sulfate Reducing ◽  
Long Run

In this study, the long-term performance and microbial dynamics of an Upflow Anaerobic Sludge Blanket (UASB) reactor targeting sulfate reduction in a SOx emissions treatment system were assessed using crude glycerol as organic carbon source and electron donor under constant S and C loading rates. The reactor was inoculated with granular sludge obtained from a pulp and paper industry and fed at a constant inlet sulfate concentration of 250 mg S-SO42−L−1 and a constant C/S ratio of 1.5 ± 0.3 g Cg−1 S for over 500 days. Apart from the regular analysis of chemical species, Illumina analyses of the 16S rRNA gene were used to study the dynamics of the bacterial community along with the whole operation. The reactor was sampled along the operation to monitor its diversity and the changes in targeted species to gain insight into the performance of the sulfidogenic UASB. Moreover, studies on the stratification of the sludge bed were performed by sampling at different reactor heights. Shifts in the UASB performance correlated well with the main shifts in microbial communities of interest. A progressive loss of the methanogenic capacity towards a fully sulfidogenic UASB was explained by a progressive wash-out of methanogenic Archaea, which were outcompeted by sulfate-reducing bacteria. Desulfovibrio was found as the main sulfate-reducing genus in the reactor along time. A progressive reduction in the sulfidogenic capacity of the UASB was found in the long run due to the accumulation of a slime-like substance in the UASB.

scholarly journals Simple estimation of granule size distribution and sludge bed porosity in a UASB reactor

2013 ◽  
Vol 10 (1) ◽  
pp. 73-79
Keyword(s):  
Size Distribution ◽  
Granular Sludge ◽  
Granule Size ◽  
Uasb Reactor ◽  
Anaerobic Sludge ◽  
Reactor Performance ◽  
Efficient Operation ◽  
Mathematical Algorithm ◽  
Bed Porosity

Granular sludge is the key factor for an efficient operation of an upflow anaerobic sludge blanket (UASB) reactor. In order to monitor the granularity of anaerobic sludge, the determination of the granule size distribution is of vital importance. Another critical parameter for the UASB reactor performance is the sludge bed porosity. For this reason, several techniques have been proposed, however they are either tedious, imprecise or expensive and hardly applicable in full scale treatment plants. There was then the need for a simple and low cost technique. This technique involves the determination of the settling velocities of a sludge sample and of extrapolating the corresponding diameters using a mathematical algorithm. In the proposed algorithm, the granules density was calculated, the flow regime was examined and finally the granule size distribution was obtained. Some very important correlations were suggested by the experimental results. The granule density and diameter as well as the sludge bed porosity were strongly correlated with the VSS/TSS ratio.

Acid Endurance and Sorption of Zn2+ of Anaerobic Granular Sludge Acclimated by Flue Gas Pretreatment Wastewater

2013 ◽  
Vol 634-638 ◽  
pp. 182-186
Author(s):  
Juan Wang ◽  
Qin Zhong
Keyword(s):  
Flue Gas ◽  
Ph Value ◽  
Granular Sludge ◽  
Upflow Anaerobic Sludge Blanket ◽  
Uasb Reactor ◽  
Anaerobic Sludge ◽  
Anaerobic Granular Sludge ◽  
Methanogenic Activity ◽  
Activity Inhibition ◽  
Anaerobic Sludge Blanket

With the aim to use anaerobic granular sludge, the methanogenic activity inhibition and recovery of anaerobic granular sludge from an industrial anaerobic reactor (s1) were investigated by measuring the methane volume at low pH. A lab-scale upflow anaerobic sludge blanket (UASB) reactor was inoculated with s1.s1 was used to remove Zn2+ in wastewater. The results show that activity of s1 is similar when the pH value is 6.5 to 7.0. The methane volume is obviously decreased when the pH value is 6.0. The activity is completely inhibited when the pH value is 4.5. The activity is fully recovered when the pH is above 6.5 and hardly recovers when the pH fell to 4.5. The main Zn2+ removal mechanism is chemical adsorption.

Advanced Reactor Design, Operation and Economy

1986 ◽  
Vol 18 (12) ◽  
pp. 99-108 ◽  
Author(s):  
Gatze Lettinga ◽  
Look Hulshoff Pol
Keyword(s):  
Suspended Solids ◽  
Granular Sludge ◽  
High Rate ◽  
Upflow Anaerobic Sludge Blanket ◽  
Uasb Reactor ◽  
Anaerobic Sludge ◽  
Internal Circulation ◽  
Inlet System ◽  
Anaerobic Sludge Blanket ◽  
Feed Inlet

Of the high rate anaerobic wastewater treatment systems the UASB (Upflow Anaerobic Sludge Blanket) reactor has found the widest application. Therefore the attention with respect to design, operation and economy will be focussed on this reactor type. In designing a UASB reactor specific attention is needed for the GSS (Gas-Solids Separator) device and the feed inlet system. For soluble wastewater generally no phase separation is required. Only for wastewaters high in suspended solids pre-acidification in a separate acidification reactor can be beneficial. Increasing attention is given to the development of modified UASB systems, such as a combination of a sludge bed reactor and an anaerobic filter. Other possible modified UASB systems may be found in a FS (Floating Settling) UASB reactor, the EGSB (Expanded Granular Sludge Bed) reactor and the UASB IC (Internal Circulation) reactor. As many factors are involved in the costs of a UASB reactor, only some rough data on reactor costs are presented.

Impact of aluminum chloride on process performance and microbial community structure of granular sludge in an upflow anaerobic sludge blanket reactor for natural rubber processing wastewater treatment

2016 ◽  
Vol 74 (2) ◽  
pp. 500-507 ◽  
Author(s):  
Nguyen Thi Thanh ◽  
Takahiro Watari ◽  
Tran Phuong Thao ◽  
Masashi Hatamoto ◽  
Daisuke Tanikawa ◽  
...  
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Natural Rubber ◽  
Microbial Community Structure ◽  
Aluminum Chloride ◽  
Granular Sludge ◽  
Upflow Anaerobic Sludge Blanket ◽  
Uasb Reactor ◽  
Anaerobic Sludge ◽  
Anaerobic Sludge Blanket

In this study, granular sludge formation was carried out using an aluminum chloride supplement in an upflow anaerobic sludge blanket (UASB) reactor treating natural rubber processing wastewater. Results show that during the first 75 days after the start-up of the UASB reactor with an organic loading rate (OLR) of 2.65 kg-COD·m−3·day−1, it performed stably with a removal of 90% of the total chemical oxygen demand (COD) and sludge still remained in small dispersed flocs. However, after aluminum chloride was added at a concentration of 300 mg·L−1 and the OLR range was increased up to 5.32 kg-COD·m−3·day−1, the total COD removal efficiency rose to 96.5 ± 2.6%, with a methane recovery rate of 84.9 ± 13.4%, and the flocs began to form granules. Massively parallel 16S rRNA gene sequencing of the sludge retained in the UASB reactor showed that total sequence reads of Methanosaeta sp. and Methanosarcina sp., reported to be the key organisms for granulation, increased after 311 days of operation. This indicates that the microbial community structure of the retained sludge in the UASB reactor at the end of the experiment gave a good account of itself in not only COD removal, but also granule formation.

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