Transfer of the broad-host-range IncQ plasmid RSF1010 and other plasmid vectors to the Gram-positive methylotroph Brevibacterium methylicum by electrotransformation

1994 ◽  
Vol 40 (6) ◽  
pp. 864-866 ◽  
Author(s):  
Jan Nešvera ◽  
Jitka Hochmannová ◽  
Miroslav Pátek ◽  
Alena Šroglová ◽  
Věra Bečvářová
Keyword(s):  
Host Range ◽  
Broad Host Range ◽  
Gram Positive ◽  
Plasmid Vectors

Transfer of the broad-host-range IncQ plasmid RSF1010 and other plasmid vectors to the Gram-positive methylotroph Brevibacterium methylicum by electrotransformationy

1994 ◽  
Vol 40 (6) ◽  
pp. 864-866 ◽  
Author(s):  
Miroslav P�tek ◽  
Alena ?roglov� ◽  
Vera Becv�rov� ◽  
Jan Ne?vera ◽  
Jitka Hochmannov�
Keyword(s):  
Host Range ◽  
Broad Host Range ◽  
Gram Positive ◽  
Plasmid Vectors

scholarly journals A Type IV-Secretion-Like System Is Required for Conjugative DNA Transport of Broad-Host-Range Plasmid pIP501 in Gram-Positive Bacteria

2007 ◽  
Vol 189 (6) ◽  
pp. 2487-2496 ◽  
Author(s):  
Mohammad Y. Abajy ◽  
Jolanta Kopeć ◽  
Katarzyna Schiwon ◽  
Michal Burzynski ◽  
Mike Döring ◽  
...  
Keyword(s):  
Host Range ◽  
Sequence Similarity ◽  
Type Iv Secretion ◽  
Conjugative Plasmid ◽  
Computer Assisted ◽  
Transcriptional Level ◽  
Broad Host Range ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Type Iv

ABSTRACT Plasmid pIP501 has a very broad host range for conjugative transfer among a wide variety of gram-positive bacteria and gram-negative Escherichia coli. Functionality of the pIP501 transfer (tra) genes in E. coli was proven by pIP501 retrotransfer to Enterococcus faecalis (B. Kurenbach, C. Bohn, J. Prabhu, M. Abudukerim, U. Szewzyk, and E. Grohmann, Plasmid 50:86-93, 2003). The 15 pIP501 tra genes are organized in a single operon (B. Kurenbach, J. Kopeć, M. Mägdefrau, K. Andreas, W. Keller, C. Bohn, M. Y. Abajy, and E. Grohmann, Microbiology 152:637-645, 2006). The pIP501 tra operon is negatively autoregulated at the transcriptional level by the conjugative DNA relaxase TraA. Three of the 15 pIP501-encoded Tra proteins show significant sequence similarity to the Agrobacterium type IV secretion system proteins VirB1, VirB4, and VirD4. Here we report a comprehensive protein-protein interaction map of all of the pIP501-encoded Tra proteins determined by the yeast two-hybrid assay. Most of the interactions were verified in vitro by isolation of the protein complexes with pull-down assays. In conjunction with known or postulated functions of the pIP501-encoded Tra proteins and computer-assisted prediction of their cellular location, we propose a model for the first type IV-secretion-like system encoded by a conjugative plasmid from gram-positive bacteria.

Characterization of Gram-positive broad host-range plasmids carrying a thermophilic replicon

1991 ◽  
Vol 142 (4) ◽  
pp. 389-396 ◽  
Author(s):  
E De Rossi ◽  
P Brigidi ◽  
M Rossi ◽  
D Matteuzzi ◽  
G Riccardi
Keyword(s):  
Host Range ◽  
Broad Host Range ◽  
Gram Positive ◽  
Broad Host Range Plasmids

Stability of r-microbes: Stabilization of plasmid vectors by the partitioning function of broad-host-range plasmid RP4

1993 ◽  
Vol 28 (2-3) ◽  
pp. 291-299 ◽  
Author(s):  
C. Haigermoser ◽  
G.Q. Chen ◽  
E. Grohmann ◽  
O. Hrabak ◽  
H. Schwab
Keyword(s):  
Host Range ◽  
Broad Host Range ◽  
Plasmid Vectors ◽  
Broad Host Range Plasmid

scholarly journals The Terminally Redundant, Nonpermuted Genome of Listeria Bacteriophage A511: a Model for the SPO1-Like Myoviruses of Gram-Positive Bacteria

2008 ◽  
Vol 190 (17) ◽  
pp. 5753-5765 ◽  
Author(s):  
Jochen Klumpp ◽  
Julia Dorscht ◽  
Rudi Lurz ◽  
Regula Bielmann ◽  
Matthias Wieland ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Host Range ◽  
Genome Structure ◽  
High Resolution Electron Microscopy ◽  
Open Reading Frames ◽  
Trna Genes ◽  
Broad Host Range ◽  
Gram Positive ◽  
Terminal Repeats ◽  
Sequence Relatedness

ABSTRACT Only little information on a particular class of myoviruses, the SPO1-like bacteriophages infecting low-G+C-content, gram-positive host bacteria (Firmicutes), is available. We present the genome analysis and molecular characterization of the large, virulent, broad-host-range Listeria phage A511. A511 contains a unit (informational) genome of 134,494 bp, encompassing 190 putative open reading frames (ORFs) and 16 tRNA genes, organized in a modular fashion common among the Caudovirales. Electron microscopy, enzymatic fragmentation analyses, and sequencing revealed that the A511 DNA molecule contains linear terminal repeats of a total of 3,125 bp, encompassing nine small putative ORFs. This particular genome structure explains why A511 is unable to perform general transduction. A511 features significant sequence homologies to Listeria phage P100 and other morphologically related phages infecting Firmicutes such as Staphylococcus phage K and Lactobacillus phage LP65. Equivalent but more-extensive terminal repeats also exist in phages P100 (∼6 kb) and K (∼20 kb). High-resolution electron microscopy revealed, for the first time, the presence of long tail fibers organized in a sixfold symmetry in these viruses. Mass spectrometry-based peptide fingerprinting permitted assignment of individual proteins to A511 structural components. On the basis of the data available for A511 and relatives, we propose that SPO1-like myoviruses are characterized by (i) their infection of gram-positive, low-G+C-content bacteria; (ii) a wide host range within the host bacterial genus and a strictly virulent lifestyle; (iii) similar morphology, sequence relatedness, and collinearity of the phage genome organization; and (iv) large double-stranded DNA genomes featuring nonpermuted terminal repeats of various sizes.

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