Fluorodeoxyglucose cell incorporation as an index of cell proliferation: evaluation of accuracy in cell culture

1993 ◽  
Vol 20 (11) ◽  
Author(s):  
D.O. Slosman ◽  
N. Pittet ◽  
A. Donath ◽  
B.S. Polla
Keyword(s):  
Cell Culture ◽  
Cell Proliferation

Artepillin C and Other Herbal PAK1-blockers: Effects on Hair Cell Proliferation and Related PAK1-dependent Biological Function in Cell Culture

2015 ◽  
Vol 30 (1) ◽  
pp. 120-127 ◽  
Author(s):  
Binh Cao Quan Nguyen ◽  
Nozomi Taira ◽  
Hiroshi Maruta ◽  
Shinkichi Tawata
Keyword(s):  
Cell Culture ◽  
Cell Proliferation ◽  
Hair Cell ◽  
Biological Function ◽  
Artepillin C

The role of matrix metalloproteinase activity in the maturation of human capillary endothelial cells in vitro

1999 ◽  
Vol 112 (10) ◽  
pp. 1599-1609 ◽  
Author(s):  
B.M. Kraling ◽  
D.G. Wiederschain ◽  
T. Boehm ◽  
M. Rehn ◽  
J.B. Mulliken ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Proliferation ◽  
Endothelial Cells ◽  
Endothelial Cell ◽  
Endothelial Cell Proliferation ◽  
Protein Levels ◽  
Matrix Deposition ◽  
Capillary Endothelial Cells ◽  
Vessel Maturation

Vessel maturation during angiogenesis (the formation of new blood vessels) is characterized by the deposition of new basement membrane and the downregulation of endothelial cell proliferation in the new vessels. Matrix remodeling plays a crucial, but still poorly understood role, in angiogenesis regulation. We present here a novel assay system with which to study the maturation of human capillary endothelial cells in vitro. When human dermal microvascular endothelial cells (HDMEC) were cultured in the presence of dibutyryl cAMP (Bt2) and hydrocortisone (HC), the deposition of a fibrous lattice of matrix molecules consisting of collagens type IV, type XVIII, laminin and thrombospondin was induced. In basal medium (without Bt2 and HC), HDMEC released active matrix metalloproteinases (MMPs) into the culture medium. However, MMP protein levels were significantly reduced by treatment with Bt2 and HC, while protein levels and activity of endogenous tissue inhibitor of MMPs (TIMP) increased. This shift in the proteolytic balance and matrix deposition was inhibited by the specific protein kinase A inhibitors RpcAMP and KT5720 or by substituting analogues without reported glucocorticoid activity for HC. The addition of MMP inhibitors human recombinant TIMP-1 or 1,10-phenanthroline to cultures under basal conditions induced matrix deposition in a dose-dependent manner, which was not observed with the serine protease inhibitor epsilon-amino-n-caproic acid (ACA). The deposited basement membrane-type of matrix reproducibly suppressed HDMEC proliferation and increased HDMEC adhesion to the substratum. These processes of matrix deposition and downregulation of endothelial cell proliferation, hallmarks of differentiating new capillaries in the end of angiogenesis, were recapitulated in our cell culture system by decreasing the matrix-degrading activity. These data suggest that our cell culture assay provides a simple and feasible model system for the study of capillary endothelial cell differentiation and vessel maturation in vitro.

Benzamide on chondrocytic differentiation in chick limb bud cell culture

Development ◽  
1985 ◽  
Vol 85 (1) ◽  
pp. 163-175
Author(s):  
Shinobu Nakanishi ◽  
Edwin M. Uyeki
Keyword(s):  
Cell Culture ◽  
Cell Proliferation ◽  
Trichloroacetic Acid ◽  
Rna Synthesis ◽  
Limb Bud ◽  
The Other ◽  
Transferase Activity ◽  
Dna And Rna ◽  
Atp Levels ◽  
Chondrocytic Differentiation

Benzamide, an inhibitor of (ADP-ribose) transferase, augmented chondrocytic differentiation of chick limb bud mesenchymal cells in micromass cultures; the incorporation of 35SO42− into the trichloroacetic-acid-insoluble constituents of cell masses as well as the formation of cartilage nodules (Nishio, Nakanishi, Doull & Uyeki, 1983) occurred about 24h earlier than in untreated cultures and continued to be enhanced in benzamide-treated cultures of stage 23- to 24-chick limb bud cells. Benzamide also significantly increased cell proliferation. However, benzamide did not affect DNA and RNA syntheses except for one period: 24 to 30 h after the start of culture, RNA synthesis was stimulated. From 48h of culture, (ADP-ribose) transferase activity decreased daily in untreated cultures, whereas benzamide treatment diminished (ADP-ribose) transferase activity 24 h earlier. On the other hand, intracellular NAD levels increased daily in untreated cultures, and benzamide significantly increased the NAD levels above untreated cultures. ATP levels did not differ significantly during the culture period, and benzamide did not affect ATP levels.

scholarly journals A Microfluidic Cell Stretch Device to Investigate the Effects of Stretching Stress on Artery Smooth Muscle Cell Proliferation in Pulmonary Arterial Hypertension

Inventions ◽  
2018 ◽  
Vol 4 (1) ◽  
pp. 1 ◽  
Author(s):  
Kae Sato ◽  
Manami Nitta ◽  
Aiko Ogawa
Keyword(s):  
Cell Culture ◽  
Cell Proliferation ◽  
Smooth Muscle ◽  
Pulmonary Arterial Hypertension ◽  
Arterial Hypertension ◽  
Smooth Muscle Cell ◽  
Muscle Cell ◽  
Cyclic Stretch ◽  
Cell Stretch ◽  
Pulmonary Arterial

A microfluidic cell stretch device was developed to investigate the effects of stretching stress on pulmonary artery smooth muscle cell (PASMC) proliferation in pulmonary arterial hypertension (PAH). The microfluidic device harbors upper cell culture and lower control channels, separated by a stretchable poly(dimethylsiloxane) membrane that acts as a cell culture substrate. The lower channel inlet was connected to a vacuum pump via a digital switch-controlled solenoid valve. For cyclic stretch at heartbeat frequency (80 bpm), the open or close time for each valve was set to 0.38 s. Proliferation of normal PASMCs and those obtained from patients was enhanced by the circumferential stretching stimulation. This is the first report showing patient cells increased in number by stretching stress. These results are consistent with the abnormal proliferation observed in PAH. Circumferential stretch stress was applied to the cells without increasing the pressure inside the microchannel. Our data may suggest that the stretch stress itself promotes cell proliferation in PAH.

scholarly journals Systems Genetics Analysis of a Recombinant Inbred Mouse Cell Culture Panel Reveals Wnt Pathway Member Lrp6 as a Regulator of Adult Hippocampal Precursor Cell Proliferation

Stem Cells ◽  
2016 ◽  
Vol 34 (3) ◽  
pp. 674-684 ◽  
Author(s):  
Suresh Kannan ◽  
Zeina Nicola ◽  
Rupert W. Overall ◽  
Muhammad Ichwan ◽  
Gerardo Ramírez-Rodríguez ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Proliferation ◽  
Recombinant Inbred ◽  
Wnt Pathway ◽  
Inbred Mouse ◽  
Mouse Cell ◽  
Precursor Cell ◽  
Systems Genetics ◽  
Recombinant Inbred Mouse

scholarly journals Investigation on the intracorneal lens material biocompatibility using the model of the corneal stromal cell culture

2017 ◽  
Vol 19 (1) ◽  
pp. 74-81 ◽  
Author(s):  
B. E. Malyugin ◽  
S. A. Borzenok ◽  
I. A. Mushkova ◽  
D. S. Ostrovskiy ◽  
I. A. Popov ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Proliferation ◽  
Stromal Cell ◽  
Plane Surface ◽  
Growth Curves ◽  
Steady Increase ◽  
Adhesive Properties ◽  
Culture Time ◽  
Stromal Cell Culture ◽  
Lens Material

Aim. To investigate cell reaction to different materials, potentially suitable for intracorneal lens (ICL) production.Materials and methods. A plane surface (2D) corneal stromal cell culture was performed in the presence of different experimental polymer samples, such as hydroxyethylmethacrylate, oligourethanemethacrylate and polymethylmethacrylate. The dynamics of cell numbers was evaluated over culture time. Conclusion on biocompatibility was made based on obtained data.Results.In each of the experimental groups there was a trend toward a steady increase in the number of cells from the 1st to 6th day of observation, the shapes of the cell growth curves showed no toxicity of materials and their ability not to interfere with cell proliferation. Cell proliferation in the contact to hydroxyethylmethacrylate and oligourethanemethacrylate materials was statistically significantly lower (p < 0.001) than in the presence of polymethylmethacrylate, which makes a strong case for their preferential usage for implantation into the stroma of the corneal optical area. When comparing the results obtained on the 6th day, the cells in the presence of the implant of hydroxyethylmethacrylate showed significantly less tendency to proliferate, than in the presence of oligourethanemethacrylate (p < 0.001). However, these differences were statistically significant not in all days.Conclusions.The results obtained have shown the absence of toxicity in experimental material samples and their low adhesive properties with respect to the stromal cell culture, thereby confirming its potential suitability for intracorneal implantation.

scholarly journals The Petri Dish-N2B27 Culture Condition Maintains RPE Phenotype by Inhibiting Cell Proliferation and mTOR Activation

2020 ◽  
Vol 2020 ◽  
pp. 1-12
Author(s):  
Hui Lou ◽  
Chunpin Lian ◽  
Fanjun Shi ◽  
Liqun Chen ◽  
Sicheng Qian ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Proliferation ◽  
Growth Medium ◽  
Culture Condition ◽  
Petri Dish ◽  
High Density ◽  
High Yield ◽  
Low Density ◽  
Rpe Cells ◽  
Petri Dishes

Objective. To develop a method for the rapid isolation of rat RPE cells with high yield and maintain its epithelial state in modified culture system. Methods. The eyeballs were incubated with dispase. The retina was isolated with RPE attached and cut into several pieces. Following a brief incubation in growth medium, large RPE sheets can be harvested rapidly. RPE cells were divided into four groups and cultured for several weeks, that is, (1) in cell culture dishes with 10% FBS containing medium (CC dish-FBS), (2) in petri dishes with 10% FBS containing medium (Petri dish-FBS), (3) in cell culture dishes with N2 and B27 containing medium (CC dish-N2B27), and (4) in petri dishes with N2 and B27 containing medium (Petri dish-N2B27). Morphological and biological characteristics were investigated using light microscopy, Q-PCR, and western blot. Results. The retina would curl inwardly during the growth medium incubation period, releasing RPE sheets in the medium. Compared with low density group (5,000 cells/cm2), RPE cells plated at high density (15,000 cells/cm2) can maintain RPE morphology for a more extended period. Meanwhile, plating RPE cells at low density significantly reduced the expression of RPE cell type-specific genes (RPE65, CRALBP, and bestrophin) and increased the expression of EMT-related genes (N-cadherin, fibronectin, and α-SMA), in comparison with the samples from the high density group. The petri dish culture condition reduced cell adhesion and thus inhibited RPE cell proliferation. As compared with other culture conditions, RPE cells in the petri dish-N2B27 condition could maintain RPE phenotype with increased expression of RPE-specific genes and decreased expression of EMT-related genes. The AKT/mTOR pathway was also decreased in petri dish-N2B27 condition. Conclusion. The current study provided an alternative method for easy isolation of RPE cells with high yield and maintenance of its epithelial morphology in the petri dish-N2B27 condition.

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