Multiple mechanisms in the smooth muscle relaxant action of calcitonin gene-related peptide (CGRP) in the guinea-pig ureter

1994 ◽  
Vol 350 (5) ◽  
Author(s):  
CarloAlberto Maggi ◽  
Sandro Giuliani ◽  
Paolo Santicioli
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Muscle Relaxant ◽  
Calcitonin Gene Related Peptide ◽  
Related Peptide ◽  
Calcitonin Gene ◽  
Smooth Muscle Relaxant

Calcitonin gene related peptide relaxes cholecystokinin-induced contraction in guinea pig gallbladder strips in vitro

1992 ◽  
Vol 70 (12) ◽  
pp. 1571-1575 ◽  
Author(s):  
L. W. Kline ◽  
P. K. T. Pang
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Calcitonin Gene Related Peptide ◽  
Intestinal Smooth Muscle ◽  
Human Calcitonin ◽  
Peptide Receptor ◽  
Related Peptide ◽  
Calcitonin Gene ◽  
Dose Dependent

Calcitonin gene related peptide has been shown to relax vascular and intestinal smooth muscle. This study examines the effects of calcitonin gene related peptide on cholecystokinin-induced contraction of guinea pig gallbladder strips in vitro. Calcitonin gene related peptide was found to cause a dose-dependent relaxation of cholecystokinin-induced tension, which was blocked by the calcitonin gene related peptide receptor antagonist human calcitonin gene related peptide8–37. Previous studies demonstrated that calcitonin gene related peptide acted directly on guinea pig gallbladder smooth muscle to inhibit acetylcholine- or KCl-induced contraction. The present results further confirm that calcitonin gene related peptide acts directly on the smooth muscle. In addition, the use of L-NG-nitroarginine methyl ester, glibenclamide, and other agents strongly suggests that calcitonin gene related peptide also acts by way of the nonadrenergic noncholinergic nervous system, to induce the relaxation of cholecystokinin-induced contraction observed in the guinea pig gallbladder strips.Key words: calcitonin gene related peptide, gallbladder, cholecystokinin.

Calcitonin gene-related peptide: am inhibitor of guinea pig gallbladder contraction

1991 ◽  
Vol 69 (8) ◽  
pp. 1149-1154 ◽  
Author(s):  
Loren W. Kline ◽  
Toyoji Kaneko ◽  
Christina G. Benishin ◽  
Peter K. T. Pang
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Colchicine Treatment ◽  
Calcitonin Gene Related Peptide ◽  
Nerve Fibers ◽  
Gallbladder Contraction ◽  
Related Peptide ◽  
Calcitonin Gene ◽  
Site Of Action

Calcitonin geae-related peptide (CGRP) relaxes vascular and intestinal smooth muscle. This study localized CGRP in the guinea pig gallbladder, examined the effects of CGRP on KCl- and ACh-induced contraction, and determined CGRPs site of action in the gallbladder. The gallbladder of male Hartley guinea pigs was used in in vitro tension studies, radioimmunoassay, or immunocytochemical studies. Radioimmunoassay showed that 8.0 ± 0.5 pmol/g of immunoreactive CGRP was present. Immunocytochemistry demonstrated that immunoreactive-CGRP nerve fibers occurred around blood vessels, in gallbladder smooth muscle layers, and were associated with ganglia. No immunoreactive cell bodies were observed, even after colchicine treatment. The in vitro tension studies showed that CGRP inhibits either KCl- or acetylcholine-stimulated contraction. CGRP may in part act directly on the gallbladder smooth muscle to inhibit contraction.Key words: calcitonin gene-related peptide, gallbladder, smooth muscle.

scholarly journals Effects of capsaicin and 5-HT3 antagonists on 5-hydroxytryptamine-evoked release of calcitonin gene-related peptide in the guinea-pig heart

1993 ◽  
Vol 108 (2) ◽  
pp. 431-435 ◽  
Author(s):  
Manuela Tramontana ◽  
Sandro Giuliani ◽  
Elena Bianco ◽  
Alessandro Lecci ◽  
Carlo A. Maggi ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Calcitonin Gene Related Peptide ◽  
Guinea Pig Heart ◽  
Related Peptide ◽  
Calcitonin Gene
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