Fluorescent dye rhodamine 6G as a molecular probe to study drug resistance of C6 rat glioma cells

1992 ◽  
Vol 13 (3) ◽  
Author(s):  
Yoshihito Matsumoto ◽  
Noboru Sasaoka ◽  
Takahiro Tsuchida ◽  
Takashi Fujiwara ◽  
Seigo Nagao ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Rhodamine 6G ◽  
Glioma Cells ◽  
Study Drug ◽  
Fluorescent Dye ◽  
Molecular Probe ◽  
Rat Glioma

effect of ginsenoside Rd on nitric oxide system induced by lipopolysaccharide plus TNF-α in C6 rat glioma cells

2003 ◽  
Vol 26 (5) ◽  
pp. 375-382 ◽  
Author(s):  
Seong-Soo Choi ◽  
Jin-Koo Lee ◽  
Eun-Jung Han ◽  
Ki-Jung Han ◽  
Han-Kyu Lee ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Glioma Cells ◽  
Oxide System ◽  
Rat Glioma ◽  
Ginsenoside Rd ◽  
Tnf Α ◽  
Nitric Oxide System

Growth inhibition by sialosyl cholesterol of rat glioma cells

1990 ◽  
Vol 17 (1) ◽  
pp. 93-100 ◽  
Author(s):  
Sumiko Abe-Dohmae ◽  
Jin-Ichi Ito ◽  
Taiji Kato ◽  
Ryo Tanaka
Keyword(s):  
Growth Inhibition ◽  
Glioma Cells ◽  
Rat Glioma

β-Adrenergic receptor desensitization stimulates glucose uptake in C6 rat glioma cells

1982 ◽  
Vol 109 (3) ◽  
pp. 753-761 ◽  
Author(s):  
Nabuyuki Shitara ◽  
Paul E. McKeever ◽  
Craig Cummins ◽  
Barry H. Smith ◽  
Paul L. Kornblith ◽  
...  
Keyword(s):  
Glucose Uptake ◽  
Adrenergic Receptor ◽  
Glioma Cells ◽  
Receptor Desensitization ◽  
Rat Glioma

scholarly journals Overexpression of CD133 Promotes Drug Resistance in C6 Glioma Cells

2010 ◽  
Vol 8 (8) ◽  
pp. 1105-1115 ◽  
Author(s):  
James M. Angelastro ◽  
Michael W. Lamé
Keyword(s):  
Drug Resistance ◽  
Glioma Cells ◽  
C6 Glioma ◽  
C6 Glioma Cells

Effect of Combined Treatment of X-rays and A CNU on Rat Glioma Cells in Monolayer and Multicellular Spheroids

1985 ◽  
Vol 25 (9) ◽  
pp. 707-714 ◽  
Author(s):  
Satoru SUGIYAMA ◽  
Teruaki MORI ◽  
Jiro SUZUKI ◽  
Takehito SASAKI
Keyword(s):  
Combined Treatment ◽  
Glioma Cells ◽  
X Rays ◽  
Multicellular Spheroids ◽  
Rat Glioma

scholarly journals Channelling of intermediates in the biosynthesis of phosphatidylcholine and phosphatidylethanolamine in mammalian cells

1998 ◽  
Vol 334 (3) ◽  
pp. 511-517 ◽  
Author(s):  
Bellinda A. BLADERGROEN ◽  
Math J. H. GEELEN ◽  
A. Ch. Pulla REDDY ◽  
Peter E. DECLERCQ ◽  
Lambert M. G. VAN GOLDE
Keyword(s):  
Staphylococcus Aureus ◽  
Mammalian Cells ◽  
Glioma Cells ◽  
Rat Hepatocytes ◽  
Cell Types ◽  
General Phenomenon ◽  
Rat Glioma ◽  
Permeabilized Cells ◽  
Metabolic Compartmentation ◽  
Choline Incorporation

Previous studies with electropermeabilized cells have suggested the occurrence of metabolic compartmentation and Ca2+-dependent channeling of intermediates of phosphatidylcholine (PC) biosynthesis in C6 rat glioma cells. With a more accessible permeabilization technique, we investigated whether this is a more general phenomenon also occurring in other cell types and whether channeling is involved in phosphatidylethanolamine (PE) synthesis as well. C6 rat glioma cells, C3H10T½ fibroblasts and rat hepatocytes were permeabilized with Staphylococcus aureus α-toxin, and the incorporation of the radiolabelled precursors choline, phosphocholine (P-choline), ethanolamine and phosphoethanolamine (P-EA) into PC and PE were measured both at high and low Ca2+ concentrations. In glioma cells, permeabilization at high Ca2+ concentration did not affect [14C]choline or [14C]P-choline incorporation into PC. However, reduction of free Ca2+ in the medium from 1.8 mM to < 1 nM resulted in a dramatic increase in [14C]P-choline incorporation into permeabilized cells, whereas [14C]choline incorporation remained unaffected. Also, in fibroblasts, reduction of extracellular Ca2+ increased [14C]P-choline and [14C]P-EA incorporation into PC and PE respectively. In hepatocytes, a combination of α-toxin and low Ca2+ concentration severely impaired [14C]choline incorporation into PC. Therefore, α-toxin-permeabilized hepatocytes are not a good model in which to study channeling of intermediates in PC biosynthesis. In conclusion, our results indicate that channeling is involved in PC synthesis in glioma cells and fibroblasts. PE synthesis in fibroblasts is also at least partly dependent on channeling.

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