Morphological changes in rat submandibular gland mucous cells during fixation with 10% formalin

1996 ◽  
Vol 253 (4-5) ◽  
Author(s):  
H. Kakoi ◽  
M. Anniko ◽  
C. Erwall ◽  
C.�.V. Pettersson ◽  
B. Jansson
Keyword(s):  
Submandibular Gland ◽  
Morphological Changes ◽  
Mucous Cells ◽  
Rat Submandibular Gland

scholarly journals Morphological Changes of Myoepithelial Cells in the Rat Submandibular Gland Following the Application of Surgical Stimuli

2016 ◽  
Vol 49 (6) ◽  
pp. 159-169 ◽  
Author(s):  
Yoshihiro Kawabe ◽  
Kenich Mizobe ◽  
Yasuhiko Bando ◽  
Koji Sakiyama ◽  
Fuyoko Taira ◽  
...  
Keyword(s):  
Submandibular Gland ◽  
Morphological Changes ◽  
Myoepithelial Cells ◽  
Rat Submandibular Gland

scholarly journals Immunohistochemical localization of Na+,K+-ATPase in rodent and human salivary and lacrimal glands.

1988 ◽  
Vol 36 (9) ◽  
pp. 1139-1145 ◽  
Author(s):  
D C Winston ◽  
R A Hennigar ◽  
S S Spicer ◽  
J R Garrett ◽  
B A Schulte
Keyword(s):  
Submandibular Gland ◽  
Immunohistochemical Localization ◽  
Secretory Cells ◽  
Mucous Cells ◽  
Duct System ◽  
Lacrimal Glands ◽  
Low Protein ◽  
Rats And Mice ◽  
Rat Submandibular Gland ◽  
Gland Type

The enzyme Na+,K+-ATPase was localized immunohistochemically in major salivary glands of mouse, rat, and human and in exorbital lacrimal glands of the rodents. Immunoreactive Na+,K+-ATPase was abundant in the basolateral membranes of all epithelial cells lining striated and intra- and interlobular ducts of all glands. Reactivity of intercalated ducts varied among gland type and species. Cells lining granular ducts in rodent submandibular gland showed a heterogeneous staining pattern in rat but stained homogeneously in mouse. Secretory cells varied greatly in their content of immunoreactive Na+,K+-ATPase. As with all duct cells, staining was present only at the basolateral surface and was never observed at the luminal surface of reactive secretory cells. Mucous cells failed to show any reactivity in any gland examined. Serous cells showed a gradient of immunostaining intensity ranging from strongly positive in demilunes of human sublingual gland to negative in rat submandibular gland and lacrimal glands of rats and mice. The presence of basolaterally localized Na+,K+-ATPase in most serous cells but not in mucous cells suggests that the enzyme contributes to the ion and water content of copious, low-protein serous secretions. The intense immunostaining of cells in most if not all segments of the duct system supports the idea that the ducts are involved with modification of the primary saliva, and extends this concept to include all segments of the duct system.

Structural and functional changes by Ciprofloxacin of rat submandibular gland

2003 ◽  
Vol 22 (4) ◽  
pp. 177-181 ◽  
Author(s):  
Mohammad Abdollahi ◽  
Bagher Minaiee ◽  
Amir A Yaaghoubi
Keyword(s):  
Submandibular Gland ◽  
Flow Rate ◽  
Total Protein ◽  
Histopathological Examination ◽  
Morphological Changes ◽  
Salivary Flow ◽  
Intracellular Camp ◽  
Functional Changes ◽  
And Function ◽  
Rat Submandibular Gland

In the present study, the effects of Ciprofloxacin (Cipro), a fluoroquinolone antibiotic, on rat submandibular gland structure and function were examined in an acute experiment. Cipro was administered intraperitoneally at various doses (20, 40 and 80 mg/kg). Pure submandibular saliva was collected intraorally by micropolyethylene tubes under anaesthesia using a dissecting microscope. After collection of saliva, submandibular glands were removed and weighed. Flow rate, amylase activity, total protein and electrolyte concentrations were measured in saliva. Concentrations of DNA and protein were measured in the gland. All doses of Cipro (20, 40, 80 mg/kg) reduced salivary flow rate. Concentrations of salivary total protein and calcium and gland DNA were reduced by all doses of Cipro. Treatment by Cipro (80 mg/kg) induced an increase in salivary sodium and potassium concentrations. Histopathological examination of glands revealed that Cipro at doses of 40 mg/kg and 80 mg/kg induces morphological changes in the glands including irregular shape of the cerous and mucous bobbles, lack of nucleus in some cells, damage of the cytoplasmic and cell walls and presence of oncocytes in secretory ducts. It is concluded that Cipro inhibits rat submandibular gland functions consistent with structural damages to the gland that might be observed as a side effect in humans. Properties of fluoroquinolones to alter intracellular cAMP and their ability to suppress DNA and protein synthesis of acinar cells might be possible reasons for observed changes.

Relationship of Fluid and Mucin Secretion to Morphological Changes in the Perfused Rat Submandibular Gland

2002 ◽  
Vol 40 (4) ◽  
pp. 203-207 ◽  
Author(s):  
M. Murakami ◽  
K. Yoshimura ◽  
H. Sugiya ◽  
Y. Seo ◽  
F. Loffredo ◽  
...  
Keyword(s):  
Submandibular Gland ◽  
Morphological Changes ◽  
Mucin Secretion ◽  
Relationship Of ◽  
Rat Submandibular Gland

Cytochemical Evidence for Presynatic β-Adrenergic Regulation of Secretion in the Developing Rat Submandibular Gland

1977 ◽  
Vol 35 ◽  
pp. 430-431
Author(s):  
L.S. Cutler
Keyword(s):  
Cyclic Amp ◽  
Adenylate Cyclase ◽  
Submandibular Gland ◽  
Neonatal Rat ◽  
Cyclase Activity ◽  
Adenylate Cyclase Activity ◽  
Parotid Glands ◽  
Adrenergic Agonist ◽  
Rat Submandibular Gland

Many studies previously have shown that the B-adrenergic agonist isoproterenol and the a-adrenergic agonist norepinephrine will stimulate secretion by the adult rat submandibular (SMG) and parotid glands. Recent data from several laboratories indicates that adrenergic agonists bind to specific receptors on the secretory cell surface and stimulate membrane associated adenylate cyclase activity which generates cyclic AMP. The production of cyclic AMP apparently initiates a cascade of events which culminates in exocytosis. During recent studies in our laboratory it was observed that the adenylate cyclase activity in plasma membrane fractions derived from the prenatal and early neonatal rat submandibular gland was retractile to stimulation by isoproterenol but was stimulated by norepinephrine. In addition, in vitro secretion studies indicated that these prenatal and neonatal glands would not secrete peroxidase in response to isoproterenol but would secrete in response to norepinephrine. In contrast to these in vitro observations, it has been shown that the injection of isoproterenol into the living newborn rat results in secretion of peroxidase by the SMG (1).

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