Levcromakalim-induced modulation of membrane potassium currents, intracellular calcium and mechanical activity in rat mesenteric artery

1994 ◽  
Vol 349 (4) ◽  
pp. 422-430 ◽  
Author(s):  
David N. Criddle ◽  
Iain A. Greenwood ◽  
Arthur H. Weston
Keyword(s):  
Intracellular Calcium ◽  
Mesenteric Artery ◽  
Potassium Currents ◽  
Mechanical Activity ◽  
Rat Mesenteric Artery

scholarly journals Alteration by lipopolysaccharide of the relationship between intracellular calcium levels and contraction in rat mesenteric artery

1996 ◽  
Vol 118 (5) ◽  
pp. 1218-1222 ◽  
Author(s):  
M. Carmen Martínez ◽  
Bernard Muller ◽  
Jean Claude Stoclet ◽  
Ramaroson Andriantsitohaina
Keyword(s):  
Intracellular Calcium ◽  
Mesenteric Artery ◽  
Rat Mesenteric Artery ◽  
The Relationship

Effects of magnesium on the vasoconstrictor responses to norepinephrine and potassium chloride in the rat mesenteric artery.

1982 ◽  
Vol 23 (5) ◽  
pp. 783-789
Author(s):  
Hiromichi SUZUKI ◽  
Kazuoki KONDO ◽  
Michiko HANDA ◽  
Takao SARUTA
Keyword(s):  
Potassium Chloride ◽  
Mesenteric Artery ◽  
Rat Mesenteric Artery

Large-conductance Ca2+-activated K+ currents blocked and impaired by homocysteine in human and rat mesenteric artery smooth muscle cells

Life Sciences ◽  
2007 ◽  
Vol 80 (22) ◽  
pp. 2060-2066 ◽  
Author(s):  
Benzhi Cai ◽  
Dongmei Gong ◽  
Zhenwei Pan ◽  
Yu Liu ◽  
Hong Qian ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Mesenteric Artery ◽  
Muscle Cells ◽  
Rat Mesenteric Artery ◽  
K Currents

Functional changes in adenylyl cyclases and associated decreases in relaxation responses in mesenteric arteries from diabetic rats

2005 ◽  
Vol 289 (5) ◽  
pp. H2234-H2243 ◽  
Author(s):  
Takayuki Matsumoto ◽  
Kentaro Wakabayashi ◽  
Tsuneo Kobayashi ◽  
Katsuo Kamata
Keyword(s):  
Mesenteric Artery ◽  
Diabetic Rats ◽  
Functional Change ◽  
Water Soluble ◽  
Mesenteric Arteries ◽  
Diabetic Rat ◽  
Adenylyl Cyclases ◽  
Camp Production ◽  
Functional Changes ◽  
Rat Mesenteric Artery

To assess the functional change in adenylyl cyclases (AC) associated with the diabetic state, we investigated AC-mediated relaxations and cAMP production in mesenteric arteries from rats with streptozotocin (STZ)-induced diabetes. The relaxations induced by the water-soluble forskolin (FSK) analog NKH477, which is a putative AC5 activator, but not by the β-adrenoceptor agonist isoproterenol (Iso) and the AC activator FSK, were reduced in intact diabetic mesenteric artery. In diabetic rats, however, Iso-, FSK-, and NKH477-induced relaxations were attenuated in the presence of inhibitors of nitric oxide synthase and cyclooxygenase. To exclude the influence of phosphodiesterase (PDE), we also examined the relaxations induced by several AC activators in the presence of 3-isobutyl-1-methylxanthine (IBMX; a PDE inhibitor). Under these conditions, the relaxation induced by Iso was greatly impaired in STZ-diabetic rats. This Iso-induced relaxation was significantly attenuated by pretreatment with SQ-22536, an AC inhibitor, in mesenteric rings from age-matched controls but not in those from STZ-diabetic rats. Under the same conditions, the relaxations induced by FSK or NKH477 were impaired in STZ-diabetic rats. Neither FSK- nor A-23187 (a Ca2+ ionophore)-induced cAMP production was significantly different between diabetics and controls. However, cAMP production induced by Iso or NKH477 was significantly impaired in diabetic mesenteric arteries. Expression of mRNAs and proteins for AC5/6 was lower in diabetic mesenteric arteries than in controls. These results suggest that AC-mediated relaxation is impaired in the STZ-diabetic rat mesenteric artery, perhaps reflecting a reduction in AC5/6 activity.

scholarly journals Sources of Ca2+ in relation to generation of acetylcholine-induced endothelium-dependent hyperpolarization in rat mesenteric artery

1997 ◽  
Vol 120 (7) ◽  
pp. 1328-1334 ◽  
Author(s):  
Mitsuhiro Fukao ◽  
Yuichi Hattori ◽  
Morio Kanno ◽  
Ichiro Sakuma ◽  
Akira Kitabatake
Keyword(s):  
Mesenteric Artery ◽  
Rat Mesenteric Artery

Orchidectomy Modulates α2-Adrenoceptor Reactivity in Rat Mesenteric Artery through Increased Thromboxane A2 Formation

2005 ◽  
Vol 43 (1) ◽  
pp. 101-108 ◽  
Author(s):  
Javier Blanco-Rivero ◽  
Gloria Balfagón ◽  
Mercedes Ferrer
Keyword(s):  
Mesenteric Artery ◽  
Rat Mesenteric Artery

scholarly journals Effects of cytochrome P450 inhibitors on potassium currents and mechanical activity in rat portal vein

1996 ◽  
Vol 119 (4) ◽  
pp. 691-701 ◽  
Author(s):  
Gillian Edwards ◽  
Peter M. Zygmunt ◽  
Edward D. Högestätt ◽  
Arthur H. Weston
Keyword(s):  
Cytochrome P450 ◽  
Portal Vein ◽  
Potassium Currents ◽  
Mechanical Activity ◽  
Rat Portal Vein ◽  
P450 Inhibitors

Abstract 202: In Vitro Effect of Trisulfate Disaccharide on ICa (L-type), INa, IK, and the Na/Ca Exchange Current Recorded From Rat Ventricular Myocytes by Patch Clamp

2020 ◽  
Vol 127 (Suppl_1) ◽  
Author(s):  
Enio R Vasques ◽  
Helena Nader ◽  
Ivarne Tersariol ◽  
Godoy Carlos
Keyword(s):  
Patch Clamp ◽  
Intracellular Calcium ◽  
Ventricular Myocytes ◽  
Potassium Currents ◽  
Ionic Currents ◽  
Exchange Current ◽  
Antiarrhythmic Action ◽  
Rat Ventricular Myocytes ◽  
Adult Rat ◽  
Vitro Effect

Background: Ion channels are pharmacological targets for antiarrhythmic action, and drugs currently used for this purpose are generally not specific to a site of action and may act on several channels and even trigger proarrhythmic phenomena. Trisulfate disaccharide (TD) is an heparin fragment known to act on the sodium calcium exchanger (NCX), reducing intracellular calcium in overload situations and reversing arrhytmias, but its action on other ionic currents is unknown. Objective: To evaluate by patch clamp the action of TD at different concentrations in NCX and ionic currents in situations of intracellular calcium overload. Materials and Methods: Adult rat myocytes were obtained from a sample from ventricles. Currents were measured using the whole-cell variant of the patch clamp method. Creation of voltage clamp pulses and data acquisition was controlled by a computer with pClamp software. Peak inward current amplitude was measured for ion currents. For Na/Ca exchange current a ramp voltage protocol was employed. Three different concentrations of Cai (300nM, 400nM and 600nM) were used in separate experiments. One drug concentration was applied per cell (10, 30 and 100 micromolar each). The current sensitive to 5mM nickel was taken as the Na/Ca exchange current. The effects of TD on the INa, L-type Ca, and the potassium currents, transiente outward current (Ito), inwardly rectifying potassium current (IK1), and sustained current (Isus) recorded from adult rat ventricular myocytes were also examined in the same conditions. Results: TD concentration-dependently increased the inward Na/Ca exchange current in all intracellular calcium concentration. The effects of TD on the INa, L-type Ca, and the potassium currents, Ito, IK1 and Isus was associated with less than 30% mean reduction on any current at the highest concentration of TD tested (100 micromolar) and still below the positive block controls for different channels that is above 40% block. Conclusion: TD acts on NCX under different concentrations used, without affecting other ionic currents, suggesting specificity in the mechanism of action and possibly not exerting a pro-arrhythmic activity, this effect being desirable for its possible use in reversal of cardiac arrhythmias.

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