Cellular lipid accumulation by Pseudomonas aeruginosa 44T1

1991 ◽  
Vol 35 (6) ◽  
pp. 813-816 ◽  
Author(s):  
C. de Andrés ◽  
M. J. Espuny ◽  
M. Robert ◽  
M. E. Mercadé ◽  
A. Manresa ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Lipid Accumulation ◽  
Cellular Lipid ◽  
Cellular Lipid Accumulation

scholarly journals Antioxidant Effect of Wheat Germ Extracts and Their Antilipidemic Effect in Palmitic Acid-Induced Steatosis in HepG2 and 3T3-L1 Cells

Foods ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1061
Author(s):  
Humna Liaqat ◽  
Kyeong Jin Kim ◽  
Soo-yeon Park ◽  
Sung Keun Jung ◽  
Sung Hee Park ◽  
...  
Keyword(s):  
Palmitic Acid ◽  
Biogenic Amines ◽  
Bioactive Compounds ◽  
Lipid Accumulation ◽  
Wheat Germ ◽  
Plasma Lipid ◽  
Reversed Phase ◽  
Antioxidant Potential ◽  
Cellular Lipid ◽  
Cellular Lipid Accumulation

Wheat germ (WG) is a by-product of wheat milling and comprises many bioactive compounds. This study aimed to compare the antioxidant and antilipidemic effects of different WG extracts (WGEs) by analyzing candidate bioactive compounds such as carotenoids, tocopherols, γ-oryzanol, and biogenic amines by reversed-phase high-performance liquid chromatography. Antioxidant activity was determined using the ABTS, DPPH, and FRAP assays. The antilipidemic effect was evaluated in palmitic acid-induced steatosis in HepG2 hepatocytes and 3T3-L1 adipocytes. Cellular lipid accumulation was assessed by Oil Red O staining and a cellular triglyceride content assay. All analyzed WGEs showed significant antioxidant potential, although some bioactive compounds, such as carotenoids, tocopherols, and γ-oryzanol, were the highest in the ethanol extract. Correlation analysis revealed the antioxidant potential of all identified biogenic amines except for spermidine. Ethanol and n-hexane extracts significantly inhibited cellular lipid accumulation in cell models. These results suggest that WGEs exhibit promising antioxidant potential, with a variety of bioactive compounds. Collectively, the findings of this study suggest that bioactive compounds in WGEs attenuate plasma lipid and oxidation levels. In conclusion, WG can be used as a natural antioxidant and nutraceutical using appropriate solvents and extraction methods.

scholarly journals ABCG1 has a critical role in mediating cholesterol efflux to HDL and preventing cellular lipid accumulation

2005 ◽  
Vol 1 (2) ◽  
pp. 121-131 ◽  
Author(s):  
Matthew A. Kennedy ◽  
Gabriel C. Barrera ◽  
Kotoka Nakamura ◽  
Ángel Baldán ◽  
Paul Tarr ◽  
...  
Keyword(s):  
Lipid Accumulation ◽  
Cholesterol Efflux ◽  
Critical Role ◽  
Cellular Lipid ◽  
Cellular Lipid Accumulation

scholarly journals Jaceosidin Inhibits Adipogenesis in 3T3-L1 Adipocytes through the PPARγ Pathway

2018 ◽  
Vol 13 (10) ◽  
pp. 1934578X1801301 ◽  
Author(s):  
Seul Gi Lee ◽  
Jongbeom Chae ◽  
Jin Soo Kim ◽  
Kyoungjin Min ◽  
Taeg Kyu Kwon ◽  
...  
Keyword(s):  
Protein Expression ◽  
Lipid Accumulation ◽  
Adipocyte Differentiation ◽  
Cellular Lipid ◽  
Mrna And Protein Expression ◽  
Anti Oxidant ◽  
Anti Inflammatory ◽  
Cellular Lipid Accumulation

Jaceosidin, a natural flavone, has been reported to have anti-tumorigenic, anti-oxidant, and anti-inflammatory activities. However, the anti-adipogenic effects of jaceosidin have not been investigated. In this study, we demonstrate that jaceosidin suppresses adipocyte differentiation and cellular lipid accumulation without cytotoxicity. Jaceosidin also suppresses the mRNA and protein expression of adipogenesis-related genes. To our knowledge, these results are the first evidence of the anti-adipogenic effects of jaceosidin in 3T3-L1 adipocytes and suggest the use of jaceosidin as a treatment for obesity.

Puerarin promotes ABCA1-mediated cholesterol efflux and decreases cellular lipid accumulation in THP-1 macrophages

2017 ◽  
Vol 811 ◽  
pp. 74-86 ◽  
Author(s):  
Cong-hui Li ◽  
Duo Gong ◽  
Ling-yan Chen ◽  
Min Zhang ◽  
Xiao-dan Xia ◽  
...  
Keyword(s):  
Lipid Accumulation ◽  
Cholesterol Efflux ◽  
Cellular Lipid ◽  
Cellular Lipid Accumulation

scholarly journals Autophagy Induction Protects against 7-Oxysterol-induced Cell Death via Lysosomal Pathway and Oxidative Stress

2016 ◽  
Vol 9 ◽  
pp. JCD.S37841 ◽  
Author(s):  
Xi-Ming Yuan ◽  
Nargis Sultana ◽  
Nabeel Siraj ◽  
Liam J. Ward ◽  
Bijar Ghafouri ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Death ◽  
Lipid Accumulation ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Autophagic Vacuole ◽  
Cellular Lipid ◽  
Autophagy Induction ◽  
Cellular Lipid Accumulation ◽  
And Oxidative Stress

7-Oxysterols are major toxic components in oxidized low-density lipoprotein and human atheroma lesions, which cause lysosomal membrane permeabilization (LMP) and cell death. Autophagy may function as a survival mechanism in this process. Here, we investigated whether 7-oxysterols mixed in an atheroma-relevant proportion induce autophagy, whether autophagy induction influences 7-oxysterol-mediated cell death, and the underlying mechanisms, by focusing on cellular lipid levels, oxidative stress, and LMP in 7-oxysterol-treated macrophages. We found that 7-oxysterols induced cellular lipid accumulation, autophagy dysfunction, and cell death in the form of both apoptosis and necrosis. Exposure to 7-oxysterols induced autophagic vacuole synthesis in the form of increased autophagy marker microtubule-associated protein 1A/1B-light chain 3 (LC3) and LC3-phosphatidylethanolamine conjugate (LC3-II) and autophagic vacuole formation. This led to an accumulation of p62, indicating a reduction in autophagic vacuole degradation. Importantly, autophagy induction significantly reduced 7-oxysterol-mediated cell death by diminishing LMP and oxidative stress. Moreover, autophagy induction minimized cellular lipid accumulation induced by 7-oxysterols. These findings highlight the importance of autophagy in combating cellular stress, LMP, and cell death in atherosclerosis. Therefore, activation of the autophagy pathway may be a potential therapeutic strategy for prevention of necrotic core formation in atherosclerotic lesions.

scholarly journals Identification of cytochrome CYP2E1 as critical mediator of synergistic effects of alcohol and cellular lipid accumulation in hepatocytes in vitro

Oncotarget ◽  
2015 ◽  
Vol 6 (39) ◽  
pp. 41464-41478 ◽  
Author(s):  
Abdo Mahli ◽  
Wolfgang E. Thasler ◽  
Eleonora Patsenker ◽  
Sebastian Müller ◽  
Felix Stickel ◽  
...  
Keyword(s):  
Lipid Accumulation ◽  
Synergistic Effects ◽  
Cellular Lipid ◽  
Cellular Lipid Accumulation
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