Molecular cloning and expression of the VP1 gene of foot-and-mouth disease virus C1 in E. coli: effect on bacterial cell viability

1991 ◽  
Vol 35 (6) ◽  
pp. 788-792 ◽  
Author(s):  
M. Vidal ◽  
J. Cairó ◽  
M. G. Mateu ◽  
A. Villaverde
Keyword(s):  
Cell Viability ◽  
Molecular Cloning ◽  
Disease Virus ◽  
Bacterial Cell ◽  
Foot And Mouth Disease ◽  
Cloning And Expression ◽  
Vp1 Gene ◽  
E Coli ◽  
Mouth Disease Virus ◽  
Bacterial Cell Viability

Molecular cloning of cDNA from foot-and-mouth disease virus C1-Santa Pau (C-S8). Sequence of protein-VP1-coding segment

Gene ◽  
1983 ◽  
Vol 23 (2) ◽  
pp. 185-194 ◽  
Author(s):  
Nieves Villanueva ◽  
Mercedes Dávila ◽  
Juan Ortín ◽  
Esteban Domingo
Keyword(s):  
Molecular Cloning ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Mouth Disease Virus ◽  
Foot And Mouth

Intracellular membrane proliferation in E. coli induced by foot-and-mouth disease virus 3A gene products

Virus Genes ◽  
1996 ◽  
Vol 12 (1) ◽  
pp. 5-14 ◽  
Author(s):  
Siegfried Weber ◽  
Harald Granzow ◽  
Frank Weiland ◽  
Otfried Marquardt
Keyword(s):  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Gene Products ◽  
Intracellular Membrane ◽  
E Coli ◽  
Mouth Disease Virus ◽  
Foot And Mouth

Cloning of cDNA of major antigen of foot and mouth disease virus and expression in E. coli

Nature ◽  
1981 ◽  
Vol 289 (5798) ◽  
pp. 555-559 ◽  
Author(s):  
Hans Küpper ◽  
Walter Keller ◽  
Christina Kurz ◽  
Sonja Forss ◽  
Heinz Schaller ◽  
...  
Keyword(s):  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
E Coli ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Major Antigen

scholarly journals CLONING AND EXPRESSION OF Lb-PROTEASE FROM cDNA CLONE OF FOOT-AND- MOUTH DISEASE VIRUS

2017 ◽  
Vol 5 (9(SE)) ◽  
pp. 62-71
Author(s):  
Swaroop Sarkar ◽  
V.V.S. Suryanarayana ◽  
S.R.Madhan Shankar
Keyword(s):  
Disease Virus ◽  
Cdna Clone ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Host Protein ◽  
Cloning And Expression ◽  
Initiation Factor ◽  
Mouth Disease Virus ◽  
N Terminus ◽  
Foot And Mouth

Foot-and –Mouth disease virus (FMDV) is a positive sense RNA virus and the genome codes for single polyprotein. The FMDV L protein is located at the N terminus of the polyprotein and is the first gene product released from the nascent polyprotein. The leader L protease which is involved in pathogenesis has two known functions: (i) auto-catalytic removal from the N terminus of the viral polyprotein and (ii) cleavage of the p220 subunit of the eukaryotic initiation factor 4F complex, which helps to shut off host protein synthesis. To explore the role of L protease in FMDV pathogenesis we generated synthetic FMDV genome lacking the L gene. The gene was amplified from an infectious cDNA clone of serotype Asia1. Primers corresponding to L protease were designed based on the sequence available in the data base. An amplified DNA of 546bp was purified and cloned into pET28 cloning vector. The sequence analysis revealed the presence of single Open Reading Frame (ORF) encoding a protein of 173 amino acid residues. The sequence alignment using BLAST search in NCBI gene Bank showed 91% homology with FMDV strain A isolate IND17/77 L protease gene. The recombinant plasmids pETLb was transferred into BL21 (DE3) pLysS cells and the IPTG induced expressed protein of 25 KDa was purified by nickel affinity column as per the manufacturer’s protocol (Sigma, USA). The specificity of the expressed protein in was confirmed by western blotting using convalescent cattle serum/ rabbit anti-bovine horse radish peroxidase conjugate and O-Dianisidine Dihydrochloride substrate.

scholarly journals Identification of optimal regions for phylogenetic studies on VP1 gene of foot-and-mouth disease virus: analysis of types A and O Argentinean viruses

2001 ◽  
Vol 32 (1) ◽  
pp. 31-45 ◽  
Author(s):  
Jose I. N��ez ◽  
Maria J. Mart�n ◽  
Maria E. Piccone ◽  
Elisa Carrillo ◽  
Eduardo L. Palma ◽  
...  
Keyword(s):  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Vp1 Gene ◽  
Phylogenetic Studies ◽  
Mouth Disease Virus ◽  
Foot And Mouth

Construction of a recombinant BHV-1 expressing the VP1 gene of foot and mouth disease virus and its immunogenicity in a rabbit model

2009 ◽  
Vol 31 (8) ◽  
pp. 1159-1165 ◽  
Author(s):  
Xian-Gang Ren ◽  
Fei Xue ◽  
Yuan-Mao Zhu ◽  
Guang-Zhi Tong ◽  
Yan-Hui Wang ◽  
...  
Keyword(s):  
Disease Virus ◽  
Rabbit Model ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Vp1 Gene ◽  
Mouth Disease Virus ◽  
Foot And Mouth
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