Modelling of cyclic fed-batch plus batch polygalacturonase production by Aureobasidium pullulans on raw orange peel

1991 ◽  
Vol 34 (6) ◽  
Author(s):  
Mauro Moresi ◽  
Maurizio Petruccioli ◽  
Federico Federici
Keyword(s):  
Aureobasidium Pullulans ◽  
Orange Peel ◽  
Fed Batch ◽  
Polygalacturonase Production

Optimization of Exo-Polygalacturonase Production from Orange Peel by Aspergillus sojae

2010 ◽  
Vol 150 ◽  
pp. 414-415 ◽  
Author(s):  
AO Buyukkileci ◽  
C Tari ◽  
HM Fernandez-Lahore ◽  
H Genckal Demir ◽  
N Gogus
Keyword(s):  
Orange Peel ◽  
Aspergillus Sojae ◽  
Polygalacturonase Production

Improved exopolysaccharide production in fed-batch fermentation of Aureobasidium pullulans, with increased impeller speed

1996 ◽  
Vol 18 (7) ◽  
pp. 787-790 ◽  
Author(s):  
Misu Moscovici ◽  
Corina Ionescu ◽  
Comeliu Oniscu ◽  
Ortansa Fotea ◽  
Paula Protopopescu ◽  
...  
Keyword(s):  
Batch Fermentation ◽  
Aureobasidium Pullulans ◽  
Impeller Speed ◽  
Fed Batch ◽  
Exopolysaccharide Production ◽  
Fed Batch Fermentation

scholarly journals Mixed glycerol and orange peel-based substrate for fed-batch microbial biodiesel production

Heliyon ◽  
2020 ◽  
Vol 6 (9) ◽  
pp. e04801
Author(s):  
Eleonora Carota ◽  
Maurizio Petruccioli ◽  
Alessandro D'Annibale ◽  
Silvia Crognale
Keyword(s):  
Orange Peel ◽  
Biodiesel Production ◽  
Fed Batch

Evaluation of Medium Composition and Fermentation Parameters on Pullulan Production by Aureobasidium pullulans

2011 ◽  
Vol 17 (2) ◽  
pp. 99-109 ◽  
Author(s):  
Kuan-Chen Cheng ◽  
Ali Demirci ◽  
Jeffrey M. Catchmark
Keyword(s):  
Production Rate ◽  
Batch Fermentation ◽  
Aureobasidium Pullulans ◽  
Fed Batch ◽  
Maximum Production ◽  
Fed Batch Fermentation ◽  
Initial Ph ◽  
Fermentation Parameters ◽  
Maximum Production Rate ◽  
Ph Profiles

The goal of this study was to enhance pullulan production by evaluating the effects of different fermentation parameters. Various carbon sources and their concentrations, yeast extract (YE) concentrations, fermentation temperatures and various pH profiles were examined. The optimal growth condition for pullulan production by Aureobasidium pullulans has been found as 75 g/L of sucrose as carbon source, 3 g/L of YE and cultivation temperature at 30 °C. Under these conditions with an initial pH at 5, 20.7 g/L of final pullulan concentration and 0.22 g/L/h maximum production rate were obtained. Later on, various pH profiles, agitation speeds, aerations and fed-batch fermentation were evaluated. The results demonstrated that pullulan production was enhanced to 25.8 g/L after 7-day cultivation with a 0.68 -g/L/h maximum production rate. There was no significant improvement of pullulan production from fed-batch fermentation. The optimal kinetics parameters were as follows: initial pH at 2.0, switched to pH 5.0 after 72 h and kept constant; agitation speed at 200 rpm; aeration at 1.5 vvm. The quality analysis demonstrated that the pullulan content produced from optimal conditions was 94.5% and its viscosity was 2.3 centipoise (cP). Fourier transform infrared spectroscopy also suggested that pullulan dominated the produced exopolysaccharide.

scholarly journals Polygalacturonase production by Aspergillus nomius MR103 in solid state fermentation using Agro-industrial wastes

2019 ◽  
Vol 11 (2) ◽  
pp. 305-310 ◽  
Author(s):  
Ravi Ketipally ◽  
G. Kranthi Kumar ◽  
M. Raghu Ram
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Enzyme Production ◽  
Orange Peel ◽  
Nitrogen Sources ◽  
Industrial Wastes ◽  
Citrus Peel ◽  
Aspergillus Nomius ◽  
Cost Efficient ◽  
Polygalacturonase Production

The present study was aimed at polygalacturonase production from Aspergillus nomius MR103 under solid state fermentation. A total of 57 fungal strains were obtained from mangrove soils collected from Gilakaladindi and Malakayalanka of Krishna District Andhra Pradesh. For the isolation of fungi these Soil samples were serially diluted and plated on pectin agar media plates.  Among them, the isolate which showed maximum polygalacturonase activity was selected for this study. This strain was identified as A. nomius MR 103 by 18S rRNA sequences analysis. Pectin rich agro-industrial wastes like apple peel, citrus peel, orange peel, wheat bran, rice bran and sugarcane bagasse were used as substrates for polygalacturonase production by A. nomius MR 103. This strain was inoculated into the nutrient broth containing agro industrial wastes under solid state fermentation and amount of Polygalacturonase production was estimated. Maximum enzyme production of 4.83 IU/mg was recorded at pH 7.0 and temperature 35?C after 7 days of incubation, when orange peels were used as substrate.  Addition of carbon and nitrogen sources to orange peel media improved the Polygalcturonase production. Sucrose as carbon and peptone as nitrogen sources were proved  to be the best for  maximum production of Polygalcturonase by A. nomius MR 103 on orange peel substrate. Utilization of agro-industrial by-products provided the establishment of a cost-efficient and sustainable process for enzyme production. 

OPTIMISATION OF MICROWAVE PRETREATMENT CONDITIONS OF ORANGE AND PLANTAIN PEELS FOR POLYGALACTURONASE PRODUCTION BY ASPERGILLUS AWAMORI CICC 2040

2021 ◽  
Vol 36 (1) ◽  
pp. 25-32
Author(s):  
O. E. Adedeji ◽  
)O. O. Ezekiel
Keyword(s):  
Particle Size ◽  
Response Surface Methodology ◽  
Orange Peel ◽  
Aspergillus Awamori ◽  
Fruit Peel ◽  
P Values ◽  
Microwave Pretreatment ◽  
Pretreatment Conditions ◽  
Polygalacturonase Production ◽  
Polygalacturonase Activity

This study investigated the optimisation of microwave pretreatment of orange and plantain peels for polygalacturonase (PG) production, by Aspergillus awamori CICC 2040, using response surface methodology. The microwave pretreatment factors interacted were particle size (PS) (<0.4250, 0.4250<PS<0.8025, and 0.8025<PS<1.1800 mm), microwave power (240, 480 and 720 W) and time (2.50, 6.25, and 10.00 min.). These factors were interacted to determine combinations for maximum polygalacturonase activity (MPA). Pretreated orange and plantain peel powders were inoculated with 106 spores/mL Aspergillus awamori CICC 2040, incubated at 28oC for 5 days, and crude polygalacturonase was extracted and its activity determined. Same microwave pretreatment combination, 0.8025<PS<1.1800 mm, 720 W and 10.00 min, gave MPA for orange and plantain peels. The MPA from orange and plantain peels was 26.21 and 26.72 U/mL, respectively. F and p values obtained for orange peel powder were 35.42 and 0.00, respectively while those obtained for plantain peel powder were 5.71 and 0.006, respectively. R2 and R2 (adjusted) of 96.96 and 94.22%, respectively were obtained for PG activity produced using orange peel powder while 90.71 and 79.04% were recorded for PG activity produced using plantain peel powder. Optimised microwave pretreatment conditions of orange and plantain peels for MPA from Aspergillus awamori CICC 2040 were established. Keywords: Aspergillus awamori, Fruit peel, Microwave, Optimisation, Polygalacturonase, Pretreatment,

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