Presynaptic ?2-adrenoceptor, opioid ?-receptor and adenosine A1-receptor interactions on noradrenaline release in rabbit brain cortex

1988 ◽  
Vol 338 (1) ◽  
Author(s):  
N. Limberger ◽  
L. Sp�th ◽  
K. Starke
Keyword(s):  
Opioid Receptor ◽  
Noradrenaline Release ◽  
Brain Cortex ◽  
Adenosine A1 Receptor ◽  
Rabbit Brain ◽  
Rabbit Brain Cortex ◽  
Receptor Interactions ◽  
Adenosine A1 ◽  
A1 Receptor

New Fluorescent Adenosine A1-Receptor Agonists That Allow Quantification of Ligand−Receptor Interactions in Microdomains of Single Living Cells

2007 ◽  
Vol 50 (4) ◽  
pp. 782-793 ◽  
Author(s):  
Richard J. Middleton ◽  
Stephen J. Briddon ◽  
Yolande Cordeaux ◽  
Andrew S. Yates ◽  
Clare L. Dale ◽  
...  
Keyword(s):  
Living Cells ◽  
Adenosine A1 Receptor ◽  
Receptor Agonists ◽  
Receptor Interactions ◽  
Adenosine A1 ◽  
A1 Receptor ◽  
Single Living Cells ◽  
Single Living

scholarly journals Characterization of adenosine A1 receptor in a cell line (28A) derived from rabbit collecting tubule

1992 ◽  
Vol 263 (2) ◽  
pp. C502-C508 ◽  
Author(s):  
W. S. Spielman ◽  
K. N. Klotz ◽  
L. J. Arend ◽  
B. A. Olson ◽  
D. G. LeVier ◽  
...  
Keyword(s):  
Cell Line ◽  
Adenosine Receptor ◽  
Binding Sites ◽  
Renal Cell ◽  
Cell Membranes ◽  
Binding Capacity ◽  
Adenosine A1 Receptor ◽  
Rabbit Brain ◽  
Adenosine A1 ◽  
A1 Receptor

We have previously reported that in several renal cell types, adenosine receptor agonists inhibit adenylyl cyclase and activate phospholipase C via a pertussis toxin-sensitive G protein. In the present study, in 28A cells, both of these adenosine receptor-mediated responses were inhibited by 8-cyclopentyl-1,3-dipropylxanthine (DPCPX), a highly selective A1 adenosine receptor antagonist. The binding characteristics of the adenosine A1 receptor in the 28A renal cell line were studied using the radiolabeled antagonist [3H]DPCPX to determine whether two separate binding sites could account for these responses. Saturation binding of [3H]DPCPX to 28A cell membranes revealed a single class of A1 binding sites with an apparent Kd value of 1.4 nM and maximal binding capacity of 64 fmol/mg protein. Competition experiments with a variety of adenosine agonists gave biphasic displacement curves with a pharmacological profile characteristic of A1 receptors. Comparison of [3H]DPCPX competition binding data from 28A cell membranes with rabbit brain membranes, a tissue with well-characterized A1 receptors, reveals that the A1 receptor population in 28A cells has similar agonist binding affinities to the receptor population in brain but has a considerably lower density. Addition of guanosine 5'-triphosphate (100 microM) to 28A cell membranes caused the competition curves to shift from biphasic to monophasic, indicating that the A1 receptors exist in two interconvertible affinity states because of their coupling to G proteins.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Adenosine A1 receptor agonist N6-cyclohexyl-adenosine induced phosphorylation of delta opioid receptor and desensitization of its signaling

2010 ◽  
Vol 31 (7) ◽  
pp. 784-790 ◽  
Author(s):  
Yun Cheng ◽  
Yi-min Tao ◽  
Jian-feng Sun ◽  
Yu-hua Wang ◽  
Xue-jun Xu ◽  
...  
Keyword(s):  
Opioid Receptor ◽  
Receptor Agonist ◽  
Adenosine A1 Receptor ◽  
Delta Opioid Receptor ◽  
Adenosine A1 ◽  
A1 Receptor
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