Release of ferulic acid from wheat bran by a ferulic acid esterase (FAE-III) from Aspergillus niger

1995 ◽  
Vol 43 (6) ◽  
pp. 1082-1087 ◽  
Author(s):  
C. B. Faulds ◽  
G. Williamson
Keyword(s):  
Aspergillus Niger ◽  
Ferulic Acid ◽  
Wheat Bran ◽  
Ferulic Acid Esterase

Production and biochemical characterization of a type B ferulic acid esterase from Streptomyces ambofaciens

2009 ◽  
Vol 55 (6) ◽  
pp. 729-738 ◽  
Author(s):  
Fadi Kheder ◽  
Stéphane Delaunay ◽  
Ghassan Abo-Chameh ◽  
Cédric Paris ◽  
Lionel Muniglia ◽  
...  
Keyword(s):  
Ferulic Acid ◽  
Wheat Bran ◽  
Biochemical Characterization ◽  
Extracellular Enzyme ◽  
Type B ◽  
Ferulic Acid Esterase ◽  
Level Of Activity ◽  
Streptomyces Ambofaciens ◽  
Methyl Ferulate ◽  
First Time

For the first time, the presence of a ferulic acid esterase (FAE) was demonstrated in Streptomyces ambofaciens . This extracellular enzyme was produced on a range of lignocellulosic substrates. The maximal level of activity was detected in the presence of either destarched wheat bran or oat spelt xylan as the sole carbon source. We found that 1% (m/v) of destarched wheat bran was the optimal concentration to induce its production. With this inducer, no ferulic acid dimers were released from the cell wall by the produced FAE. Interestingly, rape cattle cake ( Brassica napus ), which does not contain esterified ferulic acid, was also shown to induce the production of the FAE from S. ambofaciens. The FAE was partially purified from the culture supernatant. The purified enzyme was optimally active at pH 7 and 40 °C. The substrate specificity of the FAE from S. ambofaciens was investigated: the highest activity was determined with methyl p-coumarate, methyl ferulate, and methyl cinnamate. Furthermore, the FAE required a certain distance between the benzene ring and the ester bond to be active. According to these biochemical characteristics, the FAE from S. ambofaciens has been classified as a type B FAE.

scholarly journals The Effect of Xylooligosaccharide, Xylan, and Whole Wheat Bran on the Human Gut Bacteria

2020 ◽  
Vol 11 ◽  
Author(s):  
Miao Chen ◽  
Shujun Liu ◽  
Khandaker Md. Sharif Uddin Imam ◽  
Lichao Sun ◽  
Yulu Wang ◽  
...  
Keyword(s):  
Ferulic Acid ◽  
Wheat Bran ◽  
Gut Bacteria ◽  
Venn Diagram ◽  
Metagenomic Sequencing ◽  
Dietary Fibers ◽  
Ferulic Acid Esterase ◽  
Human Gut ◽  
Whole Wheat

Wheat bran is a cereal rich in dietary fibers that have high levels of ferulic acid, which has prebiotic effects on the intestinal microbiota and the host. Herein we explored the effect of xylooligosaccharide, xylan, and whole wheat bran on the human gut bacteria and screened for potential ferulic acid esterase genes. Using in vitro fermentation, we analyzed the air pressure, pH-value, and short-chain fatty acid levels. We also performed 16S rRNA gene and metagenomic sequencing. A Venn diagram analysis revealed that 80% of the core operational taxonomic units (OTUs) were shared among the samples, and most of the xylooligosaccharide treatment core OTUs (319/333 OTUs) were shared with the other two treatments’ core OTUs. A significant difference analysis revealed that the relative abundance of Dorea, Bilophila, and Sulfurovum in wheat bran treatment was higher than that in xylan and xylooligosaccharide treatments. The clusters of orthologous groups of proteins functional composition of all samples was similar to the microbiota composition of the control. Using metagenomic sequencing, we revealed seven genes containing the conserved residues, Gly-X-Ser-X-Gly, and the catalytic triad, Ser-His-Asp, which are thus potential ferulic acid esterase genes. All the results indicate that xylan and/or xylooligosaccharide, the main dietary fibers in wheat bran, plays a major role in in vitro fermentation by the human gut microbiota.

Esterification of ferulic acid with polyols using a ferulic acid esterase from Aspergillus niger

2006 ◽  
Vol 1760 (7) ◽  
pp. 1071-1079 ◽  
Author(s):  
Moriyasu Tsuchiyama ◽  
Tatsuji Sakamoto ◽  
Tomoyuki Fujita ◽  
Shuichi Murata ◽  
Haruhiko Kawasaki
Keyword(s):  
Aspergillus Niger ◽  
Ferulic Acid ◽  
Ferulic Acid Esterase

Degradation of feruloylated oligosaccharides from sugar-beet pulp and wheat bran by ferulic acid esterases from Aspergillus niger

1994 ◽  
Vol 263 (2) ◽  
pp. 257-269 ◽  
Author(s):  
Marie-Christine Ralet ◽  
Craig B. Faulds ◽  
Gary Williamson ◽  
Jean-François Thibault
Keyword(s):  
Aspergillus Niger ◽  
Sugar Beet ◽  
Ferulic Acid ◽  
Wheat Bran ◽  
Sugar Beet Pulp ◽  
Beet Pulp

Mechanistic insights into the catalytic reaction of ferulic acid decarboxylase from Aspergillus niger: a QM/MM study

2017 ◽  
Vol 19 (11) ◽  
pp. 7733-7742 ◽  
Author(s):  
Ge Tian ◽  
Yongjun Liu
Keyword(s):  
Aspergillus Niger ◽  
Ferulic Acid ◽  
Catalytic Reaction ◽  
Acid Decarboxylase ◽  
Rate Limiting Step ◽  
Limiting Step ◽  
Ferulic Acid Decarboxylase ◽  
Rate Limiting

QM/MM calculations reveal the cofactor prFMNiminiumto be the catalytically relevant species compared with prFMNketamine. The protonation of the intermediate is the rate-limiting step, and the prolonged leaving of the generated CO2can facilitate this process.

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