Influence of climatic and nutritional factors on yeast population dynamics in the phyllosphere of wheat

1992 ◽  
Vol 23 (1) ◽  
pp. 41-52 ◽  
Author(s):  
Aleid J. Dik ◽  
Nyckle J. Fokkema ◽  
Johannes A. van Pelt
Keyword(s):  
Population Dynamics ◽  
Nutritional Factors ◽  
Yeast Population

scholarly journals Viability-PCR Allows Monitoring Yeast Population Dynamics in Mixed Fermentations Including Viable but Non-Culturable Yeasts

Foods ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1373
Author(s):  
Yurena Navarro ◽  
María-Jesús Torija ◽  
Albert Mas ◽  
Gemma Beltran
Keyword(s):  
Population Dynamics ◽  
Alcoholic Fermentation ◽  
Yeast Species ◽  
Culture Media ◽  
Microbial Interactions ◽  
Fast Method ◽  
Yeast Population ◽  
Final Population ◽  
Differential Medium ◽  
Yeast Interactions

The use of controlled mixed inocula of Saccharomyces cerevisiae and non-Saccharomyces yeasts is a common practice in winemaking, with Torulaspora delbrueckii, Lachancea thermotolerans and Metschnikowia pulcherrima being the most commonly used non-Saccharomyces species. Although S. cerevisiae is usually the dominant yeast at the end of mixed fermentations, some non-Saccharomyces species are also able to reach the late stages; such species may not grow in culture media, which is a status known as viable but non-culturable (VBNC). Thus, an accurate methodology to properly monitor viable yeast population dynamics during alcoholic fermentation is required to understand microbial interactions and the contribution of each species to the final product. Quantitative PCR (qPCR) has been found to be a good and sensitive method for determining the identity of the cell population, but it cannot distinguish the DNA from living and dead cells, which can overestimate the final population results. To address this shortcoming, viability dyes can be used to avoid the amplification and, therefore, the quantification of DNA from non-viable cells. In this study, we validated the use of PMAxx dye (an optimized version of propidium monoazide (PMA) dye) coupled with qPCR (PMAxx-qPCR), as a tool to monitor the viable population dynamics of the most common yeast species used in wine mixed fermentations (S. cerevisiae, T. delbrueckii, L. thermotolerans and M. pulcherrima), comparing the results with non-dyed qPCR and colony counting on differential medium. Our results showed that the PMAxx-qPCR assay used in this study is a reliable, specific and fast method for quantifying these four yeast species during the alcoholic fermentation process, being able to distinguish between living and dead yeast populations. Moreover, the entry into VBNC status was observed for the first time in L. thermotolerans and S. cerevisiae during alcoholic fermentation. Further studies are needed to unravel which compounds trigger this VBNC state during alcoholic fermentation in these species, which would help to better understand yeast interactions.

Yeast population dynamics during prefermentative cold soak of Cabernet Sauvignon and Malbec wines

2015 ◽  
Vol 199 ◽  
pp. 23-32 ◽  
Author(s):  
Y. Paola Maturano ◽  
M. Victoria Mestre ◽  
Braulio Esteve-Zarzoso ◽  
María Cristina Nally ◽  
María Cecilia Lerena ◽  
...  
Keyword(s):  
Population Dynamics ◽  
Cabernet Sauvignon ◽  
Yeast Population

Yeast population dynamics in five spontaneous fermentations of Malvasia must

2001 ◽  
Vol 18 (3) ◽  
pp. 247-259 ◽  
Author(s):  
K. Povhe Jemec ◽  
N. Cadez ◽  
T. Zagorc ◽  
V. Bubic ◽  
A. Zupec ◽  
...  
Keyword(s):  
Population Dynamics ◽  
Yeast Population ◽  
Spontaneous Fermentations

scholarly journals Geotrichum candidumdominates in yeast population dynamics in Livarot, a French red-smear cheese

2006 ◽  
Vol 6 (8) ◽  
pp. 1243-1253 ◽  
Author(s):  
Sandra Larpin ◽  
Céline Mondoloni ◽  
Stefanie Goerges ◽  
Jean-Paul Vernoux ◽  
Micheline Guéguen ◽  
...  
Keyword(s):  
Population Dynamics ◽  
Yeast Population ◽  
Red Smear Cheese

Yeast population dynamics of industrial fuel-ethanol fermentation process assessed by PCR-fingerprinting

2005 ◽  
Vol 88 (2) ◽  
pp. 13-23 ◽  
Author(s):  
Eurípedes Alves da Silva-Filho ◽  
Scheila Karina Brito dos Santos ◽  
Alecsandra do Monte Resende ◽  
José Otamar Falcão de Morais ◽  
Marcos Antonio de Morais ◽  
...  
Keyword(s):  
Population Dynamics ◽  
Ethanol Fermentation ◽  
Fermentation Process ◽  
Fuel Ethanol ◽  
Yeast Population ◽  
Pcr Fingerprinting

Yeast population dynamics of industrial fuel-ethanol fermentation process assessed by PCR-fingerprinting

2005 ◽  
Vol 88 (1) ◽  
pp. 13-23 ◽  
Author(s):  
Eurípedes Alves da Silva-Filho ◽  
Scheila Karina Brito dos Santos ◽  
Alecsandra do Monte Resende ◽  
José Otamar Falcão de Morais ◽  
Marcos Antonio de Morais ◽  
...  
Keyword(s):  
Population Dynamics ◽  
Ethanol Fermentation ◽  
Fermentation Process ◽  
Fuel Ethanol ◽  
Yeast Population ◽  
Pcr Fingerprinting
Sign in / Sign up

Export Citation Format

Share Document