Isolation and physiological characterization of Syntrophus buswellii strain GA from a syntrophic benzoate-degrading, strictly anaerobic coculture

1995 ◽  
Vol 44 (1-2) ◽  
pp. 241-248 ◽  
Author(s):  
G. Auburger ◽  
J. Winter
Keyword(s):  
Strictly Anaerobic ◽  
Physiological Characterization

scholarly journals Post-translational thioamidation of methyl-coenzyme M reductase, a key enzyme in methanogenic and methanotrophic Archaea

2017 ◽  
Author(s):  
Dipti D. Nayak ◽  
Nilkamal Mahanta ◽  
Douglas A. Mitchell ◽  
William W. Metcalf
Keyword(s):  
Strictly Anaerobic ◽  
Methanosarcina Acetivorans ◽  
Coenzyme M ◽  
Α Subunit ◽  
Post Translational Modifications ◽  
Physiological Characterization ◽  
Key Enzyme ◽  
Catalytic Role ◽  
Methyl Coenzyme M Reductase

AbstractThe enzyme methyl-coenzyme M reductase (MCR), found in strictly anaerobic methanogenic and methanotrophic archaea, catalyzes a reversible reaction involved in the production and consumption of the potent greenhouse gas methane. The α subunit of this enzyme (McrA) contains several unusual post-translational modifications, including an exceptionally rare thioamidation of glycine. Based on the presumed function of homologous genes involved in the biosynthesis of thioamide-containing natural products, we hypothesized that the archaealtfuAandycaOgenes would be responsible for post-translational installation of thioglycine into McrA. Mass spectrometric characterization of McrA in a ΔycaO-tfuAmutant of the methanogenic archaeonMethanosarcina acetivoransrevealed the presence of glycine, rather than thioglycine, supporting this hypothesis. Physiological characterization of this mutant suggested a new role for the thioglycine modification in enhancing protein stability, as opposed to playing a direct catalytic role. The universal conservation of this modification suggests that MCR arose in a thermophilic ancestor.

Molecular and physiological characterization of Arabidopsis‐Colletotrichum gloeosporioides pathosystem

2021 ◽  
Author(s):  
Mengze Gao ◽  
Miting Wan ◽  
Liyun Yang ◽  
Meng Zhao ◽  
Xiaojin Liu ◽  
...  
Keyword(s):  
Colletotrichum Gloeosporioides ◽  
Physiological Characterization

scholarly journals Host Range of Streptomycete Strains Causing Common Scab

Plant Disease ◽  
1997 ◽  
Vol 81 (8) ◽  
pp. 901-904 ◽  
Author(s):  
Claudia Goyer ◽  
Carole Beaulieu
Keyword(s):  
Host Range ◽  
Daucus Carota ◽  
Dna Hybridization ◽  
Common Scab ◽  
Genetic Cluster ◽  
Dna Relatedness ◽  
Physiological Characterization ◽  
Genetic Clusters ◽  
High Level

Ten Streptomyces isolates from common scab lesions on carrots (Daucus carota) were characterized. Morphological and physiological characterization of the carrot isolates established that they were closely related to S. scabies. DNA-DNA hybridization studies were carried out between DNA from the carrot isolates and DNA from two potato strains belonging to the two genetic clusters of S. scabies. Most of the carrot isolates exhibited a high level of DNA relatedness (average of 90%) to strain EF-54, which belongs to genetic cluster 1 of S. scabies. Three carrot isolates could not be included in either S. scabies genetic cluster 1 or 2. The pathogenicity of six S. scabies isolates from potato or carrot, two isolates of S. caviscabies, and one isolate of S. acidiscabies was determined on potato, carrot, radish, beet, turnip, and parsnip. All S. scabies isolates were pathogenic on carrot and radish, but pathogenicity on beet, parsnip, turnip, and potato was variable. Even though S. acidiscabies and S. caviscabies until now have been isolated only from potato, we demonstrated that isolates of these species also could infect other crops, such as radish, carrot, parsnip, and turnip.

scholarly journals The Physiological Characterization of Connexin41.8 and Connexin39.4, Which Are Involved in the Striped Pattern Formation of Zebrafish

2015 ◽  
Vol 291 (3) ◽  
pp. 1053-1063 ◽  
Author(s):  
Masakatsu Watanabe ◽  
Risa Sawada ◽  
Toshihiro Aramaki ◽  
I. Martha Skerrett ◽  
Shigeru Kondo
Keyword(s):  
Pattern Formation ◽  
Striped Pattern ◽  
Physiological Characterization

Identification and Morphological-Physiological Characterization of Astaxanthin Producer Strains of Haematococcus pluvialis from the Black Sea Region

2018 ◽  
Vol 54 (6) ◽  
pp. 639-648
Author(s):  
E. S. Chelebieva ◽  
N. V. Dantsyuk ◽  
K. A. Chekanov ◽  
I. N. Chubchikova ◽  
I. V. Drobetskaya ◽  
...  
Keyword(s):  
Black Sea ◽  
Haematococcus Pluvialis ◽  
The Black Sea ◽  
Black Sea Region ◽  
Physiological Characterization ◽  
Producer Strains
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