Type beta transforming growth factor and epidermal growth factor suppress the plasminogen activator activity in a human glioblastoma cell line

1988 ◽  
Vol 6 (3) ◽  
pp. 277-283 ◽  
Author(s):  
Eirik Helseth ◽  
Are Dalen ◽  
Geirmund Unsgaard ◽  
Randi Vik
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Cell Line ◽  
Plasminogen Activator ◽  
Transforming Growth Factor ◽  
Glioblastoma Cell Line ◽  
Glioblastoma Cell ◽  
Human Glioblastoma Cell Line ◽  
Human Glioblastoma Cell ◽  
Epidermal Growth

scholarly journals Amplified gene for the epidermal growth factor receptor in a human glioblastoma cell line encodes an enzymatically inactive protein.

1988 ◽  
Vol 8 (10) ◽  
pp. 4561-4565 ◽  
Author(s):  
A Wells ◽  
J M Bishop ◽  
D Helmeste
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Cell Line ◽  
Receptor Protein ◽  
Glioblastoma Cell Line ◽  
Glioblastoma Cell ◽  
Human Glioblastoma ◽  
Human Glioblastoma Cell Line ◽  
Human Glioblastoma Cell ◽  
Epidermal Growth

The gene encoding the receptor for epidermal growth factor was amplified two- to fivefold in the human glioblastoma cell line SF268. The amplified gene gave rise to abundant quantities of receptor that bound EGF with a high affinity (Kd, 0.35 nM). The binding of ligand failed to elicit cellular DNA synthesis, however, and the receptor was enzymatically inactive. We presume that the amplified receptor gene carries a mutation(s) that affects several aspects of the receptor's function. Characterization of the mutation(s) may illuminate how structure dictates function in the receptor protein.

Amplified gene for the epidermal growth factor receptor in a human glioblastoma cell line encodes an enzymatically inactive protein

1988 ◽  
Vol 8 (10) ◽  
pp. 4561-4565
Author(s):  
A Wells ◽  
J M Bishop ◽  
D Helmeste
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Cell Line ◽  
Receptor Protein ◽  
Glioblastoma Cell Line ◽  
Glioblastoma Cell ◽  
Human Glioblastoma ◽  
Human Glioblastoma Cell Line ◽  
Human Glioblastoma Cell ◽  
Epidermal Growth

The gene encoding the receptor for epidermal growth factor was amplified two- to fivefold in the human glioblastoma cell line SF268. The amplified gene gave rise to abundant quantities of receptor that bound EGF with a high affinity (Kd, 0.35 nM). The binding of ligand failed to elicit cellular DNA synthesis, however, and the receptor was enzymatically inactive. We presume that the amplified receptor gene carries a mutation(s) that affects several aspects of the receptor's function. Characterization of the mutation(s) may illuminate how structure dictates function in the receptor protein.

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