Improved molecular weight analysis of streptococcal hyaluronic acid by size exclusion chromatography

1995 ◽  
Vol 9 (7) ◽  
pp. 491-496 ◽  
Author(s):  
David C. Armstrong ◽  
Michael R. Johns
Keyword(s):  
Molecular Weight ◽  
Hyaluronic Acid ◽  
Size Exclusion Chromatography ◽  
Size Exclusion ◽  
Exclusion Chromatography ◽  
Weight Analysis

scholarly journals Validation of an Analytical Method for the Simultaneous Determination of Hyaluronic Acid Concentration and Molecular Weight by Size-Exclusion Chromatography

Molecules ◽  
2021 ◽  
Vol 26 (17) ◽  
pp. 5360
Author(s):  
Luis Antonio Suárez-Hernández ◽  
Rosa María Camacho-Ruíz ◽  
Enrique Arriola-Guevara ◽  
Eduardo Padilla-Camberos ◽  
Manuel Reinhart Kirchmayr ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Hyaluronic Acid ◽  
Size Exclusion Chromatography ◽  
Pharmaceutical Products ◽  
Global Market ◽  
Size Exclusion ◽  
Chromatography Method ◽  
Market Growth ◽  
Exclusion Chromatography ◽  
Repeatability And Reproducibility

The hyaluronic acid (HA) global market growth can be attributed to its use in medical, cosmetic, and pharmaceutical applications; thus, it is important to have validated, analytical methods to ensure confidence and security of its use (and to save time and resources). In this work, a size-exclusion chromatography method (HPLC-SEC) was validated to determine the concentration and molecular distribution of HA simultaneously. Analytical curves were developed for concentration and molecular weight in the ranges of 100–1000 mg/L and 0.011–2.200 MDa, respectively. The HPLC-SEC method showed repeatability and reproducibility greater than 98% and limits of detection and quantification of 12 and 42 mg/L, respectively, and was successfully applied to the analysis of HA from a bacterial culture, as well as cosmetic, and pharmaceutical products.

Sign in / Sign up

Export Citation Format

Share Document