Screening of melanin biosynthesis inhibitors from marine microalgae using Streptomyces bikiniensis bioassay

1995 ◽  
Vol 9 (9) ◽  
pp. 633-636 ◽  
Author(s):  
Yoji Wachi ◽  
Koji Sode ◽  
Kenichi Horikoshi ◽  
Haruko Takeyama ◽  
Tadashi Matsunaga
Keyword(s):  
Marine Microalgae ◽  
Melanin Biosynthesis ◽  
Biosynthesis Inhibitors ◽  
Streptomyces Bikiniensis

scholarly journals A new screening method for melanin biosynthesis inhibitors using Streptomyces bikiniensis.

1990 ◽  
Vol 43 (12) ◽  
pp. 1601-1605 ◽  
Author(s):  
KOJI TOMITA ◽  
NAHOMI ODA ◽  
MASARU OHBAYASHI ◽  
HIDEO KAMEI ◽  
TAKEO MIYAKI ◽  
...  
Keyword(s):  
Screening Method ◽  
Melanin Biosynthesis ◽  
Biosynthesis Inhibitors ◽  
Streptomyces Bikiniensis

ChemInform Abstract: Pauciflorines A and B, Novel Melanin Biosynthesis Inhibitors from Kopsia.

ChemInform ◽  
2010 ◽  
Vol 27 (46) ◽  
pp. no-no
Author(s):  
T.-S. KAM ◽  
K. YOGANATHAN ◽  
T. KOYANO ◽  
K. KOMIYAMA
Keyword(s):  
Melanin Biosynthesis ◽  
Biosynthesis Inhibitors

Fungal Melanin Biosynthesis Inhibitors: Introduction of a Test System Based on the Production of Dihydroxynaphthalene (DHN) Melanin in Agar Cultures

1995 ◽  
Vol 50 (11-12) ◽  
pp. 813-819 ◽  
Author(s):  
Eckhard Thines ◽  
Thomas Daußmann ◽  
Olov Sterner ◽  
Martin Semar ◽  
Heidrun Anke
Keyword(s):  
Test System ◽  
Cytotoxic Activities ◽  
Fungal Metabolites ◽  
Melanin Biosynthesis ◽  
Biosynthesis Inhibitors ◽  
Fungal Melanin ◽  
Methoxybenzoic Acid

In a screening for inhibitors of fungal melanin biosynthesis a novel test system was success­ fully employed. With this test system, based on the production of dihydroxynaphthalene melanin (DHN melanin) by a Lachnellula species in agar cultures, extracts of cultures of basidiomycetes, ascomycetes and deuteromycetes were tested. From fermentations of Col-lybia dryophila, 6-methylpurine (1) and 6-methyl-9-β-ᴅ-ribofuranosylpurine (2) were isolated as the active principles and 3,5-dichloro-4-methoxybenzoic acid (3) was obtained from cultures of Stropharia squamosa. The corresponding alcohol (4) and aldehyde (5) previously isolated from several basidiomycetes, were also active in this test system. In a screening of fungal metabolites, 1-methoxy-8-hydroxynaphthalene and 1,8-dimethoxynaphthalene inhibited biosynthesis of DHN melanin. Among the inhibitors identified, compounds 3 -5 were the most selective ones. They were the only metabolites without cytotoxic activities.

Design of new melanin biosynthesis inhibitors

1989 ◽  
Vol 26 (3) ◽  
pp. 271-281 ◽  
Author(s):  
Kenzo Omata ◽  
Hirofumi Tomita ◽  
Tetsuo Nakajima ◽  
Bunji Natsume
Keyword(s):  
Melanin Biosynthesis ◽  
Biosynthesis Inhibitors

scholarly journals Action mechanism of the novel rice blast fungicide tolprocarb distinct from that of conventional melanin biosynthesis inhibitors

2014 ◽  
Vol 39 (3) ◽  
pp. 152-158 ◽  
Author(s):  
Takahiro Hamada ◽  
Mineko Asanagi ◽  
Tomomi Satozawa ◽  
Natsuko Araki ◽  
Shinichi Banba ◽  
...  
Keyword(s):  
Rice Blast ◽  
The Novel ◽  
Action Mechanism ◽  
Melanin Biosynthesis ◽  
Biosynthesis Inhibitors

Pauciflorines A and B, novel melanin biosynthesis inhibitors from Kopsia

1996 ◽  
Vol 37 (32) ◽  
pp. 5765-5768 ◽  
Author(s):  
Toh-Seok Kam ◽  
K Yoganathan ◽  
Takashi Koyano ◽  
Kanki Komiyama
Keyword(s):  
Melanin Biosynthesis ◽  
Biosynthesis Inhibitors

scholarly journals 1,8-Dihydroxynaphthalene (DHN)-Melanin Biosynthesis Inhibitors Increase Erythritol Production in Torula corallina, and DHN-Melanin Inhibits Erythrose Reductase

2003 ◽  
Vol 69 (6) ◽  
pp. 3427-3434 ◽  
Author(s):  
Jung-Kul Lee ◽  
Hyung-Moo Jung ◽  
Sang-Yong Kim
Keyword(s):  
Ion Exchange ◽  
Elemental Analysis ◽  
Fermentation Broth ◽  
Reductase Activity ◽  
Sugar Alcohol ◽  
Melanin Production ◽  
Melanin Biosynthesis ◽  
Biosynthesis Inhibitors ◽  
Erythrose Reductase

ABSTRACT The yeast Torula corallina is a strong erythritol producer that is used in the industrial production of erythritol. However, melanin accumulation during culture represents a serious problem for the purification of erythritol from the fermentation broth. Melanin biosynthesis inhibitors such as 3,4-dihydroxyphenylalanine and 1,8-dihydroxynaphthalene (DHN)-melanin inhibitors were added to the T. corallina cultures. Only the DHN-melanin inhibitors showed an effect on melanin production, which suggests that the melanin formed during the culturing of T. corallina is derived from DHN. This finding was confirmed by the detection of a shunt product of the pentaketide pathway, flaviolin, and elemental analysis. Among the DHN-melanin inhibitors, tricyclazole was the most effective. Supplementation with tricyclazole enhanced the production of erythritol while significantly inhibiting the production of DHN-melanin and DHN-melanin biosynthetic enzymes, such as trihydroxynaphthalene reductase. The erythrose reductase from T. corallina was purified to homogeneity by ion-exchange and affinity chromatography. Purified erythrose reductase was significantly inhibited in vitro in a noncompetitive manner by elevated levels of DHN-melanin. In contrast, the level of erythrose reductase activity was unaffected by increasing concentrations of tricyclazole. These results suggest that supplemental tricyclazole reduces the production of DHN-melanin, which may lead to a reduction in the inhibition of erythrose reductase and a higher yield of erythritol. This is the first report to demonstrate that melanin biosynthesis inhibitors increase the production of a sugar alcohol in T. corallina.

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