The nickel resistance determinant cloned from the enterobacterium Klebsiella oxytoca: conjugational transfer, expression, regulation and DNA homologies to various nickel-resistant bacteria

BioMetals ◽  
1995 ◽  
Vol 8 (1) ◽  
Author(s):  
Ralf-Dietmar Stoppel ◽  
Maria Meyer ◽  
HansG�nter Schlegel
Keyword(s):  
Klebsiella Oxytoca ◽  
Expression Regulation ◽  
Resistance Determinant ◽  
Resistant Bacteria ◽  
Nickel Resistance ◽  
Conjugational Transfer ◽  
Dna Homologies

Metabolism of methylated arsenic compounds by arsenic-resistant bacteria (Klebsiella oxytoca andXanthomonas sp.)

1992 ◽  
Vol 6 (4) ◽  
pp. 415-420 ◽  
Author(s):  
Shigeru Maeda ◽  
Akira Ohki ◽  
Kuniaki Miyahara ◽  
Kensuke Naka ◽  
Shiro Higashi
Keyword(s):  
Klebsiella Oxytoca ◽  
Resistant Bacteria ◽  
Arsenic Compounds ◽  
Arsenic Resistant Bacteria

scholarly journals Biochemical identification techniques and antibiotic susceptibility profile of lipolytic ambiental bacteria from effluents

2019 ◽  
Vol 79 (4) ◽  
pp. 555-565 ◽  
Author(s):  
A. F. G. Rave ◽  
A. V. Kuss ◽  
G. H. S. Peil ◽  
S. R. Ladeira ◽  
J. P. V. Villarreal ◽  
...  
Keyword(s):  
Antibiotic Susceptibility ◽  
Klebsiella Oxytoca ◽  
Effluent Treatment ◽  
Automated System ◽  
Resistant Bacteria ◽  
Biochemical Tests ◽  
Biochemical Identification ◽  
Identification Techniques ◽  
Susceptibility Profiles ◽  
The Impact

Abstract Different methodologies have been developed throughout the years to identify environmental microorganisms to improve bioremediation techniques, determine susceptibility profiles of bacteria in contaminated environments, and reduce the impact of microorganisms in ecosystems. Two methods of bacterial biochemical identification are compared and the susceptibility profile of bacteria, isolated from residential and industrial wastewater, is determined. Twenty-four bacteria were retrieved from the bacteria bank of the Environmental Microbiology Laboratory at the Institute of Biology (IB) of the Universidade Federal de Pelotas, Pelotas, Brazil. Bacteria were identified by conventional biochemical tests and by the VITEK ®2 automated system. Further, the susceptibility profile to antibiotics was also determined by the automated system. Six species of bacteria (Raoutella planticola, K. pneumoniae ssp. pneumoniae , Serratia marcescens, Raoutella sp., E. cloacae and Klebsiella oxytoca) were identified by conventional biochemical tests, while three species of bacteria (K. pneumoniae ssp. pneumoniae, S. marcescens and K. oxytoca ) were identified by VITEK®2 automated system. VITEK ®2 indicated agreement in 19 (79.17%) isolates and difference in five (20.83%) isolates when compared to results from conventional biochemical tests. Further, antibiotic susceptibility profile results showed that all isolates (100%) were resistant to at least one out of the 18 antibiotics tested by VITEK®2. Thus, no multi-resistant bacteria that may be used in effluent treatment systems or in bioremediation processes have been reported. Results indicate VITEK ® 2 automated system as a potential methodology in the determination of susceptibility profile and identification of environmental bacteria.

scholarly journals Neonatal sepsis in Sana’a city, Yemen: a predominance of Burkholderia cepacia

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Adeeb Salah ◽  
Ibrahim Al-Subol ◽  
Ahmed Hudna ◽  
Ali Alhaj ◽  
Abdulhabib R. Alqubaty ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Neonatal Sepsis ◽  
Antibiotic Susceptibility ◽  
Burkholderia Cepacia ◽  
Complete Blood Count ◽  
Late Onset ◽  
Klebsiella Oxytoca ◽  
Multivariable Analysis ◽  
Resistant Bacteria ◽  
Gram Positive

Abstract Background Neonatal sepsis is a global concern with increasing morbidity and mortality. The burden of neonatal sepsis is highest in developing countries, especially in those lacking proper surveillance systems. The causative pathogens and their drug-resistance levels vary between countries with emergence of multidrug resistance organisms. Thus, accurate records on the recent trends of organisms causing neonatal sepsis will provide vital information for appropriate intervention. We aimed to investigate neonatal sepsis, identify its associated factors and causative pathogens and to assess the antibiotic susceptibility patterns in Sana’a city, Yemen. Methods A cross-sectional study was conducted on neonates admitted to intensive care units of six hospitals in Sana’a city, Yemen, in the period from January 15, to March 30, 2020. Natal and prenatal medical data were collected using well-structured questionnaire. Neonates were subjected to sepsis work-up including blood culture, complete blood count and C-reactive protein. Organisms were identified by Gram staining and analyzed by the VITEK II system for bacterial bio-typing and antibiotic susceptibility testing. Findings Of the 199-neonates with suspected neonatal sepsis, 154 (77.38%) had culture-proven sepsis. Early-onset neonatal sepsis (EOS) was higher (50.25%; 100/199) than late-onset neonatal sepsis (LOS) (27.13%; 54/199). Multivariable analysis identified vaginal delivery as an independent risk factor for neonatal sepsis p = 0.005. Majority of isolated bacteria (74.39%) were gram-negative with Burkholderia cepacia (39%) and Klebsiella oxytoca (13%) being the most common pathogens of EOS and LOS. The most common gram-positive pathogens were Staphylococcus haemolyticus (9.1%) and Staphylococcus epidermidis (7.1%). B. cepacia showed multidrug resistance except for cefepime. All Klebsiella species isolates (100%) and most Pantoea species (93%) were ESBL and carbapenemase positive. All Escherichia coli and Acinetobacter baumannii isolates were ESBL positive. A significant number of gram-positive bacteria showed resistance to vancomycin. Conclusion The study findings show a high proportion of neonatal sepsis among neonates admitted to hospitals in Sana’a city with antibiotic-resistant B. cepacia being the single most common pathogen causing EOS and LOS. Findings also emphasize the emerging threat of multidrug-resistant bacteria in neonatal units and will help develop evidence-based management of neonatal sepsis in Yemen.

A Novel Nickel Resistance Determinant Found in Sewage-Associated Bacteria

1997 ◽  
Vol 33 (3) ◽  
pp. 230-239 ◽  
Author(s):  
R.W. Pickup ◽  
H.E.H. Mallinson ◽  
G. Rhodes ◽  
L.K. Chatfield
Keyword(s):  
Resistance Determinant ◽  
Nickel Resistance ◽  
Associated Bacteria

scholarly journals Molecular Analysis of Antimicrobial Resistance among Enterobacteriaceae Isolated from Diarrhoeic Calves in Egypt

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1712
Author(s):  
Abdel-Moamen E. Meshref ◽  
Ibrahim E. Eldesoukey ◽  
Abdulaziz S. Alouffi ◽  
Saleh A. Alrashedi ◽  
Salama A. Osman ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Klebsiella Oxytoca ◽  
Conventional Polymerase Chain Reaction ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
M Gene ◽  
Resistant Species ◽  
Antimicrobial Resistance Genes ◽  
Class 1 ◽  
Shigella Spp

The present study was designed to investigate the presence of genes that conferred resistance to antimicrobials among Enterobacteriaceae that were isolated from diarrhoeic calves. A total of 120 faecal samples were collected from diarrhoeic calves that were raised in Kafr El-Sheikh governorate, Egypt. The samples were screened for Enterobacteriaceae. A total of 149 isolates of bacteria were recovered and identified; Escherichia coli was found to be the most overwhelming species, followed by Citrobacter diversus, Shigella spp., Serratia spp., Providencia spp., Enterobacter spp., Klebsiella pneumoniae, Proteus spp., Klebsiella oxytoca, and Morganella morganii. All isolates were tested for susceptibility to 12 antimicrobials; resistant and intermediately resistant strains were screened by conventional polymerase chain reaction for the presence of antimicrobial resistance genes. Of the 149 isolates, 37 (24.8%) exhibited multidrug resistant phenotypes. The most prevalent multidrug resistant species were E. coli, C. diversus, Serratia spp., K. pneumoniae, Shigella spp., Providencia spp., and K. oxytoca. Class 1 integrons were detected in 28 (18.8%) isolates. All isolates were negative for class 2 integrons. The blaTEM gene was identified in 37 (24.8%) isolates, whereas no isolates carried the blaCTX-M gene. The florfenicol gene (floR) was detected in two bacterial isolates (1.3%). The findings of this study reveal that calves may act as potential reservoirs of multidrug resistant bacteria that can be easily transmitted to humans.

scholarly journals The Occurrence of Potentially Pathogenic and Antibiotic Resistant Gram-Negative Bacteria Isolated from the Danube Delta Ecosystem

2021 ◽  
Vol 13 (7) ◽  
pp. 3955
Author(s):  
Alina R. Banciu ◽  
Daniela L. Ionica ◽  
Monica A. Vaideanu ◽  
Dragos M. Radulescu ◽  
Mihai Nita-Lazar ◽  
...  
Keyword(s):  
Global Warming ◽  
Surface Water ◽  
Klebsiella Oxytoca ◽  
Coliform Bacteria ◽  
Transport Systems ◽  
Resistant Bacteria ◽  
Close Monitoring ◽  
Danube Delta ◽  
Beta Lactam ◽  
Antibiotic Resistant

The spread of a growing number of antibiotic-resistant bacteria (ARB) outside the clinical setting into the environment has been observed. The surface water plays an important role in ARB dissemination by being both habitats and transport systems for microorganisms. The ecological and touristic importance of the Danube Delta make it a European priority for close monitoring of its freshwater system. The main goal of this paper was to analyze how the St. Gheorghe branch of the Danube Delta microbiological contamination and their antibiotic-resistant profile were influenced by climate change, especially the global warming from 2013 up to 2019. In the surface water from all sampling points, total and fecal coliform bacteria showed a constant colony forming units (CFU) increase tendency during the years, with a sharp rise from 1500 CFU/mL in 2015 to more than 20,000 CFU/mL in 2019. The bacterial population’s analyses revealed an indirect proportionality between coliform bacteria density in water and sediment during the years in accordance with global warming. The most commonly identified bacterial strains such as Escherichia coli, Klebsiella oxytoca, Citrobacter freundii and Proteus mirabilis have been shown a resistance rate of approximatively 70% to beta-lactam antibiotics, especially to ampicillin and amoxicillin-clavulanate.

scholarly journals Rapid Detection of New Delhi Metallo-β-Lactamase Gene Using Recombinase-Aided Amplification Directly on Clinical Samples From Children

2021 ◽  
Vol 12 ◽  
Author(s):  
Yanling Feng ◽  
Guanhua Xue ◽  
Junxia Feng ◽  
Chao Yan ◽  
Jinghua Cui ◽  
...  
Keyword(s):  
Klebsiella Oxytoca ◽  
Multi Drug Resistance ◽  
Clinical Samples ◽  
Resistant Bacteria ◽  
New Delhi ◽  
Analytical Sensitivity ◽  
Acinetobacter Junii ◽  
Carbapenem Resistant ◽  
Three Samples ◽  
Lactamase Gene

New Delhi metallo-β-lactamase, a metallo-β-lactamase carbapenemase type, mediates resistance to most β-lactam antibiotics including penicillins, cephalosporins, and carbapenems. Therefore, it is important to detect blaNDM genes in children’s clinical samples as quickly as possible and analyze their characteristics. Here, a recombinase-aided amplification (RAA) assay, which operates in a single one-step reaction tube at 39°C in 5−15 min, was established to target blaNDM genes in children’s clinical samples. The analytical sensitivity of the RAA assay was 20 copies, and the various bacterial types without blaNDM genes did not amplify. This method was used to detect blaNDM genes in 112 children’s stool samples, 10 of which were tested positive by both RAA and standard PCR. To further investigate the characteristics of carbapenem-resistant bacteria carrying blaNDM in children, 15 carbapenem-resistant bacteria (Escherichia coli, Klebsiella pneumoniae, Acinetobacter baumannii, Citrobacter freundii, Klebsiella oxytoca, Acinetobacter junii, and Proteus mirabilis) were isolated from the 10 samples. Notably, more than one bacterial type was isolated from three samples. Most of these isolates were resistant to cephalosporins, cefoperazone-sulbactam, piperacillin-tazobactam, ticarcillin-clavulanic acid, aztreonam, co-trimoxazole, and carbapenems. blaNDM–1 and blaNDM–5 were the two main types in these samples. These data show that the RAA assay has potential to be a sensitive and rapid blaNDM gene screening test for clinical samples. The common existence of blaNDM and multi-drug resistance genes presents major challenges for pediatric treatment.

Antibiotic susceptibility profile of extended spectrum β-lactamase producing Escherichia coli, Klebsiella pneumoniae and Klebsiella oxytoca from Urinary tract infections

2021 ◽  
pp. 4425-4428
Author(s):  
M. Sharmal Kumar ◽  
Arunagirinathan N. ◽  
Ravikumar M.
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Antibiotic Susceptibility ◽  
Klebsiella Oxytoca ◽  
Bacterial Pathogen ◽  
Resistant Bacteria ◽  
Extended Spectrum ◽  
High Level ◽  
Tract Infections

The aim of this study was to analyze the extended spectrum β-lactamases (ESBLs) production and antibiotic susceptibility profile of urinary tract infected bacterial pathogens such as Escherichia coli and Klebsiella spp. A total of 143 Gram-negative bacteria were isolated from people suffering from urinary tract infections (UTIs) were included in this study. Among them, Escherichia coli (75%) were the predominantly isolated bacterial pathogen followed by Klebsiella oxytoca (14.6%) and K. pneumoniae (10.4%). Approximately 65% (n=93) of isolates were positive for ESBL production and E.coli was found to be the highest ESBL producer (67.6%) followed by K. oxytoca (57.1%) and K. pneumoniae (53.3%). E. coli showed high level of 86.1% resistance to cefotaxime and cefuroxime and 100% sensitive to imipenem and meropenem, whereas, K. oxytoca showed high level of 90.5% resistance to cefuroxime and 100% sensitive to amikacin, imipenem and meropenem. Similarly, K. pneumoniae showed high level of 73.3% resistance to nitrofurantoin and 93.3% sensitive to imipenem. This study reveals that majority of UTIs caused bacteria are ESBL producing multidrug-resistant bacteria and showing broad spectrum antibiotic resistance profile.

scholarly journals Occurrence of Plasmid- and Chromosome-Carried mcr-1 in Waterborne Enterobacteriaceae in China

2017 ◽  
Vol 61 (8) ◽  
Author(s):  
Hong-Wei Zhou ◽  
Ting Zhang ◽  
Ji-Hua Ma ◽  
Ying Fang ◽  
Han-Yu Wang ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Klebsiella Oxytoca ◽  
Culture Method ◽  
Environmental Water ◽  
Broth Culture ◽  
Resistant Bacteria ◽  
Enrichment Broth ◽  
Content Type ◽  
Sampling Locations ◽  
Susceptibility Profiles

ABSTRACT The aim of this study was to investigate the prevalence of the polymyxin resistance gene mcr-1 in Enterobacteriaceae from environmental water sources in Hangzhou, China. Colistin-resistant bacteria were isolated from environmental water samples using an enrichment broth culture method, were screened for mcr-1, and then were analyzed for the location and transferability of mcr-1. Isolates positive for mcr-1 were further examined to determine their susceptibility profiles and were screened for the presence of additional resistance genes. Twenty-three mcr-1-positive isolates (16 Escherichia coli, two Citrobacter freundii, two Klebsiella oxytoca, two Citrobacter braakii, and one Enterobacter cloacae) were isolated from 7/9 sampling locations; of those, eight mcr-1-positive isolates also contained β-lactamase-resistance genes, eight contained qnrS, and 10 contained oqx. No mcr-2-positive isolates were identified. The majority of isolates demonstrated a low to moderate level of colistin resistance. Transconjugation was successfully conducted from 14 of the 23 mcr-1-positive isolates, and mcr-1 was identified on plasmids ranging from 60 to 220 kb in these isolates. Conjugation and hybridization experiments revealed that mcr-1 was chromosome-borne in only three isolates. Pulsed-field gel electrophoresis showed that the majority of E. coli isolates belonged to different clonal lineages. Multilocus sequence typing analysis revealed that sequence type 10 (ST10) was the most prevalent, followed by ST181 and ST206. This study demonstrates the utility of enrichment broth culture for identifying environmental mcr-1-positive isolates. Furthermore, it highlights the importance of responsible agriculture and clinical use of polymyxins to prevent further widespread dissemination of polymyxin-resistant pathogens.

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