A cell retention internal filter reactor for ethanol production using tapioca hydrolysates

1994 ◽  
Vol 8 (11) ◽  
pp. 817-820 ◽  
Author(s):  
Woo Gi Lee ◽  
Yong-Seok Lee ◽  
Ho Nam Chang ◽  
Yong Keun Chang
Keyword(s):  
Ethanol Production ◽  
Cell Retention ◽  
A Cell

Continuous ethanol production from wood hydrolysate by chemostat and total cell retention culture

1996 ◽  
Vol 13 (5) ◽  
pp. 453-456 ◽  
Author(s):  
Woo Gi Lee ◽  
Jin Suk Lee ◽  
Byung Geon Park ◽  
Mi Sun Kim ◽  
Soon Chul Park ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Total Cell ◽  
Cell Retention ◽  
Wood Hydrolysate

scholarly journals Report of unexpected findings after cardiac stem cell injections in a preclinical model

2021 ◽  
Author(s):  
Mira van der Naald ◽  
Hans T van den Broek ◽  
John LM Bemelmans ◽  
Klaus Neef ◽  
Maarten H Bakker ◽  
...  
Keyword(s):  
Ischemia Reperfusion ◽  
Future Research ◽  
Preclinical Model ◽  
Cardiac Cell ◽  
Cell Retention ◽  
Cell Accumulation ◽  
A Cell ◽  
Indium 111 ◽  
Total Body ◽  
Unexpected Findings

Introduction Cardiac regenerative therapy is a proposed therapy for ischemic heart disease. So far efficacy has been low and this might partly be explained by low cardiac cell retention. In this study we aimed to investigate if cardiac cell retention improves using ureido-pyrimidinone units (UPy-gel) as a cell carrier. Methods We used an ischemia-reperfusion model. Pigs were randomized to intramyocardial injections with mesenchymal stromal cells (MSC) labelled with both Indium-111 and a fluorescent tracer in either PBS or in the UPy-gel. After 4 hours, a total body scintigraphy was performed to determine the cardiac cell retention and histology was obtained. Results In the first 4 pigs, we noticed focused areas of radio activity (hotspots) outside the heart in both the control and UPy-gel arm, and decided to interrupt the study. At histology we confirmed one hotspots to be located in a lymph node. No satisfactory explanation for these, potentially harmful, hotspots was found. Conclusion This study was interrupted due to unexpected extra-cardiac hotspots. Although we do not have a conclusive explanation for these findings, we find that sharing these results is important for future research. We recommend to use total body imaging in future retention studies to confirm of reject the occurrence of extra-cardiac cell accumulation after intramyocardial cell injection and discover the pathophysiology and its clinical implications.

scholarly journals Developing a Cell-Free Extract Reaction (CFER) System in Clostridium thermocellum to Identify Metabolic Limitations to Ethanol Production

2020 ◽  
Vol 8 ◽  
Author(s):  
Jingxuan Cui ◽  
David Stevenson ◽  
Travis Korosh ◽  
Daniel Amador-Noguez ◽  
Daniel G. Olson ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Clostridium Thermocellum ◽  
Cell Free Extract ◽  
A Cell

scholarly journals Carbon Starvation Can Induce Energy Deprivation and Loss of Fermentative Capacity in Saccharomyces cerevisiae

2003 ◽  
Vol 69 (6) ◽  
pp. 3251-3257 ◽  
Author(s):  
Elisabeth Thomsson ◽  
Christer Larsson ◽  
Eva Albers ◽  
Annika Nilsson ◽  
Carl Johan Franz�n ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ethanol Production ◽  
Nitrogen Starvation ◽  
Carbon Starvation ◽  
Adaptation Period ◽  
Fermentative Capacity ◽  
Ethanol Production Rate ◽  
A Cell ◽  
Different Strains ◽  
Energy Deprivation

ABSTRACT Seven different strains of Saccharomyces cerevisiae were tested for the ability to maintain their fermentative capacity during 24 h of carbon or nitrogen starvation. Starvation was imposed by transferring cells, exponentially growing in anaerobic batch cultures, to a defined growth medium lacking either a carbon or a nitrogen source. After 24 h of starvation, fermentative capacity was determined by addition of glucose and measurement of the resulting ethanol production rate. The results showed that 24 h of nitrogen starvation reduced the fermentative capacity by 70 to 95%, depending on the strain. Carbon starvation, on the other hand, provoked an almost complete loss of fermentative capacity in all of the strains tested. The absence of ethanol production following carbon starvation occurred even though the cells possessed a substantial glucose transport capacity. In fact, similar uptake capacities were recorded irrespective of whether the cells had been subjected to carbon or nitrogen starvation. Instead, the loss of fermentative capacity observed in carbon-starved cells was almost surely a result of energy deprivation. Carbon starvation drastically reduced the ATP content of the cells to values well below 0.1 μmol/g, while nitrogen-starved cells still contained approximately 6 μmol/g after 24 h of treatment. Addition of a small amount of glucose (0.1 g/liter at a cell density of 1.0 g/liter) at the initiation of starvation or use of stationary-phase instead of log-phase cells enabled the cells to preserve their fermentative capacity also during carbon starvation. The prerequisites for successful adaptation to starvation conditions are probably gradual nutrient depletion and access to energy during the adaptation period.

Repeated-batch production of kojic acid in a cell-retention fermenter using Aspergillus oryzae M3B9

2005 ◽  
Vol 32 (6) ◽  
pp. 227-233 ◽  
Author(s):  
H. M. Wan ◽  
C. C. Chen ◽  
R. Giridhar ◽  
T. S. Chang ◽  
W. T. Wu
Keyword(s):  
Aspergillus Oryzae ◽  
Kojic Acid ◽  
Batch Production ◽  
Cell Retention ◽  
A Cell ◽  
Repeated Batch
Sign in / Sign up

Export Citation Format

Share Document