Increased capillary permeability in rat brain induced by factors secreted by cultured C6 glioma cells: role in peritumoral brain edema

1991 ◽  
Vol 10 (1) ◽  
pp. 13-25 ◽  
Author(s):  
Takanori Ohnishil ◽  
Peter B. Sher ◽  
Jerome B. Posner ◽  
William R. Shapiro ◽  
George C. Cotzias
Keyword(s):  
Rat Brain ◽  
Brain Edema ◽  
Capillary Permeability ◽  
Glioma Cells ◽  
C6 Glioma ◽  
C6 Glioma Cells ◽  
Peritumoral Brain Edema

scholarly journals TGF-β1 EXPRESSION BY GLIOMA C6 CELLS IN VITRO

2017 ◽  
Vol 39 (4) ◽  
pp. 258-263 ◽  
Author(s):  
L D Liubich ◽  
L M Kovalevska ◽  
M I Lisyany ◽  
V M Semenova ◽  
T A Malysheva ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Rat Brain ◽  
Glioma Cells ◽  
C6 Glioma ◽  
C6 Glioma Cells ◽  
C6 Cells ◽  
Fetal Rat ◽  
Tgf Β1 ◽  
Fetal Rat Brain

The aim of the work was to study the impact of fetal rat brain cell supernatant (FRBCS) on the expression of transforming growth factor β1 (TGF-β1) and p53 in C6 cells of rat glioma in vitro. Materials and Methods: FRBCS was obtained from suspensions of fetal rat brain cells on the 14th (E14) day of gestation. C6 glioma cells were cultured for 48 h in the presence of FRBCS or FRBCS + anti-TGF-β1 monoclonal antibody. Immunocytochemical staining for TGF-β1 and p53 was performed. Results: The proportion of TGF-β1-immunopositive tumor cells in C6 glioma cultures was statistically significantly higher than in the control cell cultures of normal fetal rat brain. FRBCS reduced the proportion of TGF-β1-immunopositive tumor cells and increased the proportion of p53-immunopositive cells in C6 glioma cultures. In cells cultured with FRBCS + anti-TGF-β1 monoclonal antibody, the above effects of FRBCS were abrogated. Conclusion: The obtained results suggest that TGF-β1 seems to be responsible for decrease in TGF-β1 expression and increase in p53 expression in C6 glioma cells treated with FRBCS.

scholarly journals Effect of Docosahexaenoic Acid on the Synthesis of Phosphatidylserine in Rat Brain Microsomes and C6 Glioma Cells

2002 ◽  
Vol 70 (1) ◽  
pp. 24-30 ◽  
Author(s):  
Martha C. Garcia ◽  
Glenn Ward ◽  
Yee-Chung Ma ◽  
Norman Salem ◽  
Hee-Yong Kim
Keyword(s):  
Docosahexaenoic Acid ◽  
Rat Brain ◽  
Glioma Cells ◽  
C6 Glioma ◽  
C6 Glioma Cells ◽  
Brain Microsomes

Invasion of experimental rat brain tumor: early morphological changes following microinjection of C6 glioma cells

1993 ◽  
Vol 86 (2) ◽  
pp. 117-125 ◽  
Author(s):  
Nobuhisa Nagano ◽  
Hiroshi Sasaki ◽  
Masaru Aoyagi ◽  
Kimiyoshi Hirakawa
Keyword(s):  
Brain Tumor ◽  
Rat Brain ◽  
Morphological Changes ◽  
Glioma Cells ◽  
C6 Glioma ◽  
C6 Glioma Cells ◽  
Rat Brain Tumor

Reduced tumorigenicity of rat glioma cells in the brain when mediated by hygromycin phosphotransferase

2001 ◽  
Vol 94 (4) ◽  
pp. 596-604 ◽  
Author(s):  
Adília Hormigo ◽  
David R. Friedlander ◽  
Perry A. Brittis ◽  
David Zagzag ◽  
Martin Grumet
Keyword(s):  
Cell Proliferation ◽  
Rat Brain ◽  
Glioma Cells ◽  
C6 Glioma ◽  
C6 Glioma Cells ◽  
C6 Cells ◽  
Content Type ◽  
Hygromycin Phosphotransferase ◽  
The Brain ◽  
Fine Print

Object. A variant of C6 glioma cells, C6R-G/H cells express hygromycin phosphotransferase (HPT) and appear to have reduced tumorigenicity in the embryonic brain. The goal of this study was to investigate their reduced capacity to generate tumors in the adult rat brain. Methods. Cell lines were implanted into rat brains and tumorigenesis was evaluated. After 3 weeks, all rats with C6 cells showed signs of neurological disease, whereas rats with C6R-G/H cells did not and were either killed then or allowed to survive until later. Histological studies were performed to analyze tumor size, malignancy, angiogenesis, and cell proliferation. Cells isolated from rat brain tumors were analyzed for mutation to HPT by testing their sensitivity to hygromycin. Conclusions. The results indicate that HPT suppresses tumor formation. Three weeks after implantation, only 44% of animals implanted with C6R-G/H cells developed tumors, whereas all animals that received C6 glioma cells developed high-grade gliomas. The C6R-G/H cells filled a 20-fold smaller maximal cross-sectional area than the C6 cells, and exhibited less malignant characteristics, including reduced angiogenesis, mitosis, and cell proliferation. Similar results were obtained in the brain of nude rats, indicating that the immune system did not play a significant role in suppressing tumor growth. The combination of green fluorescent protein (GFP) and HPT was more effective in suppressing tumorigenesis than either plasmid by itself, indicating that the GFP may protect against inactivation of the HPT. Interestingly, hygromycin resistance was lost in tumor cells that were recovered from a group of animals in which C6R-G/H cells formed tumors, confirming the correlation of HPT with reduced tumorigenicity.

Individual C6 glioma cells migrate in adult rat brain after neural homografting

1991 ◽  
Vol 9 (4) ◽  
pp. 427-433 ◽  
Author(s):  
William J. Goldberg ◽  
Edward R. Laws ◽  
Jerald J. Bernstein
Keyword(s):  
Rat Brain ◽  
Glioma Cells ◽  
C6 Glioma ◽  
C6 Glioma Cells ◽  
Adult Rat ◽  
Adult Rat Brain

Expression of the rat brain creatine kinase gene in C6 glioma cells

1993 ◽  
Vol 35 (1) ◽  
pp. 92-102 ◽  
Author(s):  
C. D. Wilson ◽  
B. Parameswaran ◽  
G. R. Molloy
Keyword(s):  
Creatine Kinase ◽  
Rat Brain ◽  
Glioma Cells ◽  
C6 Glioma ◽  
C6 Glioma Cells ◽  
Kinase Gene ◽  
Brain Creatine Kinase

Receptor-mediated uptake of β-glucuronidase into primary astrocytes and C6 glioma cells from rat brain

1988 ◽  
Vol 462 (2) ◽  
pp. 265-274 ◽  
Author(s):  
Huw G. Jenkins ◽  
Jane Martin ◽  
Michael F. Dean
Keyword(s):  
Rat Brain ◽  
Glioma Cells ◽  
C6 Glioma ◽  
C6 Glioma Cells ◽  
Primary Astrocytes

Volume regulation and metabolism of suspended C6 glioma cells: An in vitro model to study cytotoxic brain edema

1983 ◽  
Vol 279 (1-2) ◽  
pp. 217-228 ◽  
Author(s):  
O. Kempski ◽  
L. Chaussy ◽  
U. Gross ◽  
M. Zimmer ◽  
A. Baethmann
Keyword(s):  
Brain Edema ◽  
Volume Regulation ◽  
In Vitro Model ◽  
Glioma Cells ◽  
C6 Glioma ◽  
C6 Glioma Cells ◽  
Vitro Model
Sign in / Sign up

Export Citation Format

Share Document