The effect of the desert cobra (Walterinnesia aegyptia) crude venom and its protein fractions on the metabolic activity of cultured human fibroblasts

1996 ◽  
Vol 12 (3) ◽  
pp. 127-134 ◽  
Author(s):  
S. S. Al-Saleh
Keyword(s):  
Metabolic Activity ◽  
Human Fibroblasts ◽  
Protein Fractions ◽  
Crude Venom ◽  
Cultured Human Fibroblasts

scholarly journals The Effect ofWalterinnesia aegyptiaVenom Proteins on TCA Cycle Activity and Mitochondrial NAD+-Redox State in Cultured Human Fibroblasts

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Hazem K. Ghneim ◽  
Yazeed A. Al-Sheikh ◽  
Mourad A. M. Aboul-Soud
Keyword(s):  
Enzyme Activities ◽  
Redox State ◽  
Human Fibroblasts ◽  
Tca Cycle ◽  
Similar Magnitude ◽  
Crude Venom ◽  
Control Activity ◽  
Cycle Enzyme ◽  
Cultured Human Fibroblasts ◽  
Venom Proteins

Fibroblast cultures were used to study the effects of crudeWalterinnesia aegyptiavenom and its F1–F7 protein fractions on TCA cycle enzyme activities and mitochondrial NAD-redox state. Confluent cells were incubated with 10 μg of venom proteins for 4 hours at 37°C. The activities of all studied TCA enzymes and the non-TCA mitochondrial NADP+-dependent isocitrate dehydrogenase underwent significant reductions of similar magnitude (50–60% of control activity) upon incubation of cells with the crude venom and fractions F4, F5, and F7 and 60–70% for fractions F3 and F6. In addition, the crude and fractions F3–F7 venom proteins caused a drop in mitochondrial NAD+and NADP+levels equivalent to around 25% of control values. Whereas the crude and fractions F4, F5, and F7 venom proteins caused similar magnitude drops in NADH and NADPH (around 55% of control levels), fractions F3 and F6 caused a more drastic drop (60–70% of control levels) of both reduced coenzymes. Results indicate that the effects of venom proteins could be directed at the mitochondrial level and/or the rates of NAD+and NADP+biosynthesis.

scholarly journals Influence of chlorate on proteoglycan biosynthesis by cultured human fibroblasts.

1988 ◽  
Vol 263 (26) ◽  
pp. 12886-12892 ◽  
Author(s):  
H Greve ◽  
Z Cully ◽  
P Blumberg ◽  
H Kresse
Keyword(s):  
Human Fibroblasts ◽  
Cultured Human Fibroblasts

scholarly journals Fatty alcohol metabolism in cultured human fibroblasts. Evidence for a fatty alcohol cycle.

1987 ◽  
Vol 262 (36) ◽  
pp. 17412-17419 ◽  
Author(s):  
W B Rizzo ◽  
D A Craft ◽  
A L Dammann ◽  
M W Phillips
Keyword(s):  
Fatty Alcohol ◽  
Human Fibroblasts ◽  
Alcohol Metabolism ◽  
Cultured Human Fibroblasts

L-iduronidase in cultured human fibroblasts and liver

1971 ◽  
Vol 42 (2) ◽  
pp. 340-345 ◽  
Author(s):  
Reuben Matalon ◽  
J.A. Cifonelli ◽  
Albert Dorfman
Keyword(s):  
Human Fibroblasts ◽  
Cultured Human Fibroblasts

scholarly journals The transport of neutral amino acids in cultured human fibroblasts.

1980 ◽  
Vol 255 (3) ◽  
pp. 929-936 ◽  
Author(s):  
G.C. Gazzola ◽  
V. Dall'Asta ◽  
G.G. Guidotti
Keyword(s):  
Amino Acids ◽  
Human Fibroblasts ◽  
Neutral Amino Acids ◽  
Cultured Human Fibroblasts

scholarly journals Transport of l-Isoleucine by Cultured Human Fibroblasts

1974 ◽  
Vol 249 (11) ◽  
pp. 3430-3435
Author(s):  
Richard E. Hillman ◽  
Elaine F. Otto
Keyword(s):  
Human Fibroblasts ◽  
Cultured Human Fibroblasts

scholarly journals Na/K/Cl cotransport in cultured human fibroblasts.

1985 ◽  
Vol 260 (3) ◽  
pp. 1445-1451
Author(s):  
N E Owen ◽  
M L Prastein
Keyword(s):  
Human Fibroblasts ◽  
Cultured Human Fibroblasts
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