Pollination with gamma-irradiated pollen and development of fruits, seeds and parthenogenetic plants in apple

Euphytica ◽  
1991 ◽  
Vol 54 (1) ◽  
pp. 101-109 ◽  
Author(s):  
Y. X. Zhang ◽  
Y. Lespinasse
Keyword(s):  
Irradiated Pollen ◽  
Gamma Irradiated

Seed yield and quality in Populus tremuloides following pollination with gamma-irradiated pollen

1978 ◽  
Vol 56 (23) ◽  
pp. 2967-2972
Author(s):  
T. D. Rudolph
Keyword(s):  
Seed Yield ◽  
Populus Tremuloides ◽  
Seed Quality ◽  
Quaking Aspen ◽  
Quality Factors ◽  
Yield And Quality ◽  
Irradiated Pollen ◽  
Pollen Mixture ◽  
The Mean ◽  
Gamma Irradiated

A pollen mixture from three male quaking aspen (Populus tremuloides Michx.) trees was irradiated at exposures of 484, 968, 1453, 1937, 3874, 7747, and 15 494 R and used to control-pollinate cut branches from three female trees. The pollen radiosensitivity was determined from various seed yield and seed quality factors. The pollen LD50 exposure varied with the end point evaluated, ranging from 255 R for number of 50-mesh seed per catkin to 8800 R for total seeds per catkin. The mean LD50 for nine seed yield and seed quality end points was 3995 R. A significant stimulatory response in seed yield was noted at low pollen irradiation levels, particularly at the 484-R exposure. The LD100 was approached but not reached at 15 494 R. Irradiated quaking aspen pollen may be useful in breeding experiments.

scholarly journals Studies on Citrus pollination using gamma-irradiated pollen

1988 ◽  
Vol 54 (3) ◽  
pp. 257-264 ◽  
Author(s):  
J.H. de Lange ◽  
A.P. Vincent
Keyword(s):  
Irradiated Pollen ◽  
Gamma Irradiated

scholarly journals Production of Haploids in Persian Walnut through Parthenogenesis Induced by Gamma-irradiated Pollen

2011 ◽  
Vol 136 (3) ◽  
pp. 198-204 ◽  
Author(s):  
Mohammad Sadat Hosseini Grouh ◽  
Kourosh Vahdati ◽  
Mahmoud Lotfi ◽  
Darab Hassani ◽  
Nejat Pirvali Biranvand
Keyword(s):  
Embryo Rescue ◽  
Juglans Regia ◽  
Breeding Programs ◽  
Persian Walnut ◽  
Irradiated Pollen ◽  
Molecular Marker Analysis ◽  
Gamma Irradiated ◽  
Haploid Plants

We report the first successful regeneration of haploid lines in persian walnut (Juglans regia) developed by in situ parthenogenesis followed by embryo rescue. Female flowers of cultivars Hartley and Pedro and two native Iranian selections (Z63 and Z67) were pollinated using pollen of selections Z53 and Z30 that had been irradiated with gamma rays at five doses (50, 150, 300, 600, and 900 Gy). Gamma-irradiated pollen induced fruit set and development of some parthenogenetic embryos. The immature embryos were excised 30 and 45 days after pollination, cultured in vitro, and then stratified for 30 days at 4 °C to overcome dormancy. Ploidy level of the resulting plantlets was determined by chromosome counting and flow cytometry. Haploid plants were obtained from ‘Hartley’, ‘Pedro’, Z63, and Z67 after pollination using pollen irradiated at 300 and 600 Gy. Plants obtained from pollen irradiated at 50 and 150 Gy were all diploid. Molecular marker analysis using four simple sequence repeat (SSR) markers also showed that all the diploid plants recovered were zygotic and no spontaneous double haploid plants were obtained in this work. Also, the haploid plantlets presented only one allele of their female parents. These profiles confirmed the parthenogenetic origin of the obtained haploid plants. The techniques used to induce haploid walnut plants by irradiated pollen were successful and could be used in breeding programs and accelerate genome analysis in this plant in which the genome size is approximately three times the size of the human genome.

scholarly journals Effect of gamma ray irradiated pollen technique on seed development pattern in Citrus

2020 ◽  
Vol 80 (04) ◽  
Author(s):  
Manoj Kundu ◽  
Anil Dubey
Keyword(s):  
Gamma Ray ◽  
Developmental Pattern ◽  
Breeding Cycle ◽  
Major Drawback ◽  
Irradiated Pollen ◽  
Irradiation Treatment ◽  
Normal Seed ◽  
Gamma Irradiated ◽  
Higher Doses ◽  
Healthy Seed

In modern fruit breeding, to reduce the breeding cycle, induction of haploid plants through irradiated pollen technique is of paramount importance. However, the major drawback is the sensitivity of pollen to higher irradiation doses as it induces abnormality in double fertilization process. Hence, optimization of irradiation doses for maximum recovery of healthy seed is very important. Present work analyzed the seed developmental pattern of Citrus grandis, pollinated with gamma irradiated pollen (100-500 Gy) of C. limon, C. limetta, and C. sinensis. Not a single fruit was retained on the tree till maturity in C. grandis × C. limon crosses; however, in other two cross combinations, fruits were harvested at maturity only up to 300 Gy irradiation doses. Among the harvested fruit, normal seed decreased gradually with increasing irradiation doses and less than 5 normal seeds per fruit were obtained at 300 Gy with maximum in C. grandis × C. limetta cross. However, abnormal and empty number no. of seeds has increased significantly with increasing irradiation doses. Developmental pattern of embryo and endosperm within normal seeds was also influenced significantly by irradiation treatment. At higher doses, seeds number with both embryo and endosperm was decreased gradually and at 300 Gy, it was reduced to 59.47% as compared to control.

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