Purification by high performance liquid chromatography of Clostridium perfringens type a enterotoxin prepared from high toxin producers selected by a toxin-antitoxin halo

1985 ◽  
Vol 1 (2) ◽  
pp. 131-138 ◽  
Author(s):  
N. Sugimoto ◽  
K. Ozutsumi ◽  
M. Matsuda
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Clostridium Perfringens ◽  
High Performance ◽  
Type A ◽  
Toxin Producers

scholarly journals Purification of Clostridium perfringens enterotoxins by high performance liquid chromatography

1985 ◽  
Vol 29 (3) ◽  
pp. 293-297 ◽  
Author(s):  
Takashi Uemura ◽  
Yumiko Aoi ◽  
Yasuhiko Horiguchi ◽  
Akira Wadano ◽  
Naoki Goshima ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Clostridium Perfringens ◽  
High Performance

scholarly journals Identification of Type A, B, E, and F Botulinum Neurotoxin Genes and of Botulinum Neurotoxigenic Clostridia by Denaturing High-Performance Liquid Chromatography

2004 ◽  
Vol 70 (7) ◽  
pp. 4170-4176 ◽  
Author(s):  
Giovanna Franciosa ◽  
Manoocheher Pourshaban ◽  
Alessandro De Luca ◽  
Anna Buccino ◽  
Bruno Dallapiccola ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Botulinum Toxin ◽  
High Performance ◽  
Botulinum Neurotoxin ◽  
Type A ◽  
Food Borne Pathogens ◽  
Food Borne ◽  
Pcr Products ◽  
Dhplc Analysis

ABSTRACT Denaturing high-performance liquid chromatography (DHPLC) is a recently developed technique for rapid screening of nucleotide polymorphisms in PCR products. We used this technique for the identification of type A, B, E, and F botulinum neurotoxin genes. PCR products amplified from a conserved region of the type A, B, E, and F botulinum toxin genes from Clostridium botulinum, neurotoxigenic C. butyricum type E, and C. baratii type F strains were subjected to both DHPLC analysis and sequencing. Unique DHPLC peak profiles were obtained with each different type of botulinum toxin gene fragment, consistent with nucleotide differences observed in the related sequences. We then evaluated the ability of this technique to identify botulinal neurotoxigenic organisms at the genus and species level. A specific short region of the 16S rRNA gene which contains genus-specific and in some cases species-specific heterogeneity was amplified from botulinum neurotoxigenic clostridia and from different food-borne pathogens and subjected to DHPLC analysis. Different peak profiles were obtained for each genus and species, demonstrating that the technique could be a reliable alternative to sequencing for the rapid identification of food-borne pathogens, specifically of botulinal neurotoxigenic clostridia most frequently implicated in human botulism.

888: Metabolomic Urine Samples Profiling Using High Performance Liquid Chromatography Coupled with Mass Spectrometry to Detect Bladder Cancer

2007 ◽  
Vol 177 (4S) ◽  
pp. 295-295
Author(s):  
Michael Mullerad ◽  
Haleem J. Issaq ◽  
Alexander Kravtsov ◽  
Timothy Waybright ◽  
Brian Luke ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Bladder Cancer ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Urine Samples
Sign in / Sign up

Export Citation Format

Share Document