scholarly journals Role of fungi in cyanogens removal during solid substrate fermentation of cassava

1994 ◽  
Vol 16 (7) ◽  
pp. 755-758 ◽  
Author(s):  
A. J. Alexander Essers ◽  
Claudia M. J. W. Witjes ◽  
Erna W. Schurink ◽  
M. J. Robert Nout
Biomolecules ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 98
Author(s):  
Lidia Błaszczyk ◽  
Agnieszka Waśkiewicz ◽  
Karolina Gromadzka ◽  
Katarzyna Mikołajczak ◽  
Jerzy Chełkowski

The occurrence and diversity of Lecanicillium and Sarocladium in maize seeds and their role in this cereal are poorly understood. Therefore, the present study aimed to investigate Sarocladium and Lecanicillium communities found in endosphere of maize seeds collected from fields in Poland and their potential to form selected bioactive substances. The sequencing of the internally transcribed spacer regions 1 (ITS 1) and 2 (ITS2) and the large-subunit (LSU, 28S) of the rRNA gene cluster resulted in the identification of 17 Sarocladium zeae strains, three Sarocladium strictum and five Lecanicillium lecanii isolates. The assay on solid substrate showed that S. zeae and S. strictum can synthesize bassianolide, vertilecanin A, vertilecanin A methyl ester, 2-decenedioic acid and 10-hydroxy-8-decenoic acid. This is also the first study revealing the ability of these two species to produce beauvericin and enniatin B1, respectively. Moreover, for the first time in the present investigation, pyrrocidine A and/or B have been annotated as metabolites of S. strictum and L. lecanii. The production of toxic, insecticidal and antibacterial compounds in cultures of S. strictum, S. zeae and L. lecanii suggests the requirement to revise the approach to study the biological role of fungi inhabiting maize seeds.


1983 ◽  
Vol 3 (5) ◽  
pp. 931-945
Author(s):  
B D Crawford ◽  
J C Barrett ◽  
P O Ts'o

Analysis of the role of gene mutations in the multistep process of neoplastic transformation requires that the discrete steps in carcinogenesis first be dissected. Toward this end, we have isolated and characterized preneoplastic Syrian hamster cells which exhibit in vitro a trait highly correlated with neoplastic conversion in vivo. Previous findings (J. C. Barrett, Cancer Res. 40:91-94, 1980) indicate that spontaneous neoplastic transformation of Syrian hamster cells occurs in at least two steps. An intermediate stage, characterized by an aneuploid established cell line which has a propensity to become neoplastic spontaneously upon further growth in vitro, has been described. These preneoplastic cells differ from diploid early-passage Syrian hamster cells in becoming capable of anchorage-independent growth in semisolid agar, as well as becoming neoplastic in vivo when attached to a solid substrate. Evidence presented here demonstrates that anchorage-independent conversion in vitro is a reliable marker for neoplastic conversion in this cell system. Fluctuation analyses, patterned after those described by Luria and Delbruck for microbial genetics, demonstrate that anchorage-independent variants are generated randomly from clonally derived preneoplastic cells at the rate of 10(-8) to 10(-7) variants per cell per generation. These results establish a multistep stochastic process for transformation in vitro and indicate that conversion to anchorage independence may be necessary for Syrian hamster cells to become tumorigenic. The possible role of gene mutation in this step during neoplastic progression is discussed.


1984 ◽  
Vol 6 (2) ◽  
pp. 97-102 ◽  
Author(s):  
E. Maheva ◽  
G. Djelveh ◽  
C. Larroche ◽  
J. B. Gros

1987 ◽  
Vol 29 (8) ◽  
pp. 962-968 ◽  
Author(s):  
P. Daubresse ◽  
S. Ntibashirwa ◽  
A. Gheysen ◽  
J. A. Meyer

2008 ◽  
Vol 4 (1) ◽  
pp. 36-46 ◽  
Author(s):  
M. Daker ◽  
N. Abdullah ◽  
S. Vikineswar ◽  
U.R. Kuppusamy

2015 ◽  
Vol 10 (10) ◽  
pp. 1934578X1501001 ◽  
Author(s):  
Xianfu Wu ◽  
Yadan Wang ◽  
Shuchun Liu ◽  
Xinzhong Liu ◽  
Liangdong Guo

Three new ambuic acid derivatives, microsporols A-C (1-3) and the known compound ambuic acid (4), were isolated from the solid-substrate fermentation cultures of the plant endophytic fungus Pestalotiopsis microspora. Their structures were elucidated primarily by NMR experiments. The absolute configurations of the 6,7-diol moiety in 1 and 2 were assigned using the Snatzke's method, whereas that of 3 was deduced by circular dichroism (CD) exciton chirality method. Compounds 1, 3, and 4 showed moderate 5-lipoxygenase (5-LOX) inhibitory effects.


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