Enhanced alcohol yields in batch cultures of Clostridium acetobutylicum using a three-electrode potentiometric system with methyl viologen as electron carrier

1994 ◽  
Vol 16 (3) ◽  
pp. 269-274 ◽  
Author(s):  
S. Peguin ◽  
P. Delorme ◽  
G. Goma ◽  
P. Soucaille
Keyword(s):  
Clostridium Acetobutylicum ◽  
Methyl Viologen ◽  
Electron Carrier ◽  
Batch Cultures

Electrochemical Study of Reversible Hydrogenase Reaction ofDesulfovibrio vulgarisCells with Methyl Viologen as an Electron Carrier

1999 ◽  
Vol 71 (9) ◽  
pp. 1753-1759 ◽  
Author(s):  
Hirosuke Tatsumi ◽  
Kazuyoshi Takagi ◽  
Megumi Fujita ◽  
Kenji Kano ◽  
Tokuji Ikeda
Keyword(s):  
Methyl Viologen ◽  
Electrochemical Study ◽  
Electron Carrier ◽  
Reversible Hydrogenase

scholarly journals d-2,3-Butanediol Production Due to Heterologous Expression of an Acetoin Reductase in Clostridium acetobutylicum

2011 ◽  
Vol 77 (8) ◽  
pp. 2582-2588 ◽  
Author(s):  
Marco A. J. Siemerink ◽  
Wouter Kuit ◽  
Ana M. López Contreras ◽  
Gerrit Eggink ◽  
John van der Oost ◽  
...  
Keyword(s):  
Experimental Data ◽  
Heterologous Expression ◽  
Metabolic Network ◽  
Clostridium Acetobutylicum ◽  
Clostridium Beijerinckii ◽  
Racemic Mixture ◽  
Gene Encoding ◽  
Content Type ◽  
Batch Cultures

ABSTRACTAcetoin reductase (ACR) catalyzes the conversion of acetoin to 2,3-butanediol. Under certain conditions,Clostridium acetobutylicumATCC 824 (and strains derived from it) generates bothd- andl-stereoisomers of acetoin, but because of the absence of an ACR enzyme, it does not produce 2,3-butanediol. A gene encoding ACR fromClostridium beijerinckiiNCIMB 8052 was functionally expressed inC. acetobutylicumunder the control of two strong promoters, the constitutivethlpromoter and the late exponentialadcpromoter. Both ACR-overproducing strains were grown in batch cultures, during which 89 to 90% of the natively produced acetoin was converted to 20 to 22 mMd-2,3-butanediol. The addition of a racemic mixture of acetoin led to the production of bothd-2,3-butanediol andmeso-2,3-butanediol. A metabolic network that is in agreement with the experimental data is proposed. Native 2,3-butanediol production is a first step toward a potential homofermentative 2-butanol-producing strain ofC. acetobutylicum.

Photoinduced hydrogen evolution with lysine-linked viologen and hydrogenase

2002 ◽  
Vol 06 (01) ◽  
pp. 26-32 ◽  
Author(s):  
Noriyuki Asakura ◽  
Akimitsu Miyaji ◽  
Toshiaki Kamachi ◽  
Ichiro Okura
Keyword(s):  
Hydrogen Evolution ◽  
Electron Donor ◽  
Methyl Viologen ◽  
Evolution System ◽  
Electron Carrier ◽  
Effective Hydrogen

Lysine-linked viologen was prepared as a substrate for the hydrogenase. By using reduced lysine-linked viologen ( LysV +•), the hydrogenase- LysV +• complex was formed efficiently, leading to effective hydrogen evolution compared with methyl viologen. Lysine-linked viologen as an electron carrier was applied for the photoinduced hydrogen evolution system containing hydrogenase, Tetrakis(4-carboxyphenyl)porphyrin ( TCPP ) as a photosensitizer, and a sacrificial electron donor for TCPP . In this system, effective photoinduced hydrogen evolution was observed.

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