Substrate adhesiveness and experimental metastatic potential of rat ascites hepatoma AH7974-derived variant sublines

1992 ◽  
Vol 10 (4) ◽  
Author(s):  
Takanori Kawaguchi ◽  
Seiji Igarashi ◽  
Hironao Wakabayashi ◽  
Sachihiko Yokoya ◽  
Kiyoshi Fukui
Keyword(s):  
Metastatic Potential ◽  
Ascites Hepatoma ◽  
Rat Ascites Hepatoma ◽  
Experimental Metastatic Potential

scholarly journals Inhibition of P-Glycoprotein-Dependent Multidrug Resistance by an Isoquinolinesulfonamide Compound H-87 in Rat Ascites Hepatoma AH66 Cells.

1996 ◽  
Vol 19 (6) ◽  
pp. 886-889 ◽  
Author(s):  
Shigeo NAKAMURA ◽  
Tomoyoshi MINAMINO ◽  
Masaaki NOMURA ◽  
Shinya WAKUSAWA ◽  
Ken-ichi MIYAMOTO ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Ascites Hepatoma ◽  
P Glycoprotein ◽  
Rat Ascites Hepatoma

Cell surface heparan sulphate and adhesive property of sublines of rat ascites hepatoma AH7974

1988 ◽  
Vol 90 (4) ◽  
pp. 683-689 ◽  
Author(s):  
A. Kimura ◽  
T. Kawaguchi ◽  
T. Ono ◽  
A. Sakuma ◽  
Y. Yokoya ◽  
...  
Keyword(s):  
Cell Surface ◽  
Culture Medium ◽  
Heparan Sulphate ◽  
Soluble Form ◽  
Foetal Calf Serum ◽  
Serum Free Medium ◽  
Free Medium ◽  
Ascites Hepatoma ◽  
Serum Free ◽  
Rat Ascites Hepatoma

Two variants (74AD and 74FL) established from rat ascites hepatoma AH7974 were examined for the production of glycosaminoglycans in culture. There was no difference between the adhesive (74AD) and the floating (74FL) variants in quantity of glycosaminoglycans produced by their cultivation in minimum essential medium supplemented with 10% foetal calf serum. However, they were distinctly different in the distribution patterns of heparan sulphate. In 74FL, about 70% of total heparan sulphate was found in the culture medium in soluble form, whereas in 74AD, only 7% was found in the medium and the rest was in the cell-substratum complex. In a serum-free medium, 74AD cells grew without adhering to the substratum. After cultivation, more than 90% of total heparan sulphate was found in the cell-associated fractions and the rest in the substratum fractions. No heparan sulphate was detected in the culture medium. On the other hand, 74FL cells released heparan sulphate to the serum-free medium as much as to the serum-containing medium. The increase in amount of heparan sulphate in the culture medium of 74FL cells was supposed to be caused by failure of the cells to deposit heparan sulphate at the cell surface and not caused by increased production. Cell-substratum adhesion mechanisms involving cell surface heparan sulphate (heparan sulphate proteoglycan) and some serum intermediate(s) are discussed for 74AD cells.

Comparative Study of Glycolipid Compositions of Plasma Membranes among Two Types of Rat Ascites Hepatoma and Normal Rat Liver

1978 ◽  
Vol 83 (5) ◽  
pp. 1517-1520 ◽  
Author(s):  
Takao TAKI ◽  
Yoshio HIRABAYASHI ◽  
Yoshiko SUZUKI ◽  
Makoto MATSUMOTO ◽  
Kiyohide KOJIMA
Keyword(s):  
Comparative Study ◽  
Rat Liver ◽  
Plasma Membranes ◽  
Ascites Hepatoma ◽  
Normal Rat ◽  
Rat Ascites Hepatoma

scholarly journals Loss of hormonal control of glycogenolysis in rat ascites hepatoma cells

1986 ◽  
Vol 40 ◽  
pp. 107
Author(s):  
Kenichi Miyamoto ◽  
Shinya Wakusawa ◽  
Toshikazu Yanaoka ◽  
Ryozo Koshiura
Keyword(s):  
Hepatoma Cells ◽  
Hormonal Control ◽  
Ascites Hepatoma ◽  
Rat Ascites Hepatoma

scholarly journals Purification and characterization of nuclear alkaline phospholipase A2 in rat ascites hepatoma cells

FEBS Letters ◽  
1996 ◽  
Vol 394 (1) ◽  
pp. 55-60 ◽  
Author(s):  
Takashi Oishi ◽  
Keiko Tamiya-Koizumi ◽  
Ichiro Kudo ◽  
Satoshi Iino ◽  
Kenzo Takagi ◽  
...  
Keyword(s):  
Phospholipase A2 ◽  
Hepatoma Cells ◽  
Ascites Hepatoma ◽  
Purification And Characterization ◽  
Rat Ascites Hepatoma

Cell membrane polarity in rat ascites hepatoma cells

1985 ◽  
Vol 240 (2) ◽  
Author(s):  
Yasuji Ishimaru ◽  
Ryuichi Hattori ◽  
Chen Jinyan ◽  
Hideo Hayashi
Keyword(s):  
Cell Membrane ◽  
Hepatoma Cells ◽  
Ascites Hepatoma ◽  
Rat Ascites Hepatoma

Some Chemical and Immunological Analyses of Proteins of Rat Ascites Hepatoma*

1961 ◽  
Vol 49 (6) ◽  
pp. 682-692 ◽  
Author(s):  
HIDEMATUS HIRAI ◽  
KOYA SEKINE ◽  
AKIKO IIJIMA ◽  
KOTARO WARABIOKA
Keyword(s):  
Ascites Hepatoma ◽  
Rat Ascites Hepatoma
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