Increased shikonin production by hairy roots of Lithospermum erythrorhizon in two phase bubble column reactor

1993 ◽  
Vol 15 (2) ◽  
pp. 145-150 ◽  
Author(s):  
Sang Jun Sim ◽  
Ho Nam Chang
Keyword(s):  
Hairy Roots ◽  
Bubble Column ◽  
Bubble Column Reactor ◽  
Lithospermum Erythrorhizon ◽  
Two Phase ◽  
Column Reactor

Batch and Fed-Batch Production of Betalains by Red Beet (Beta vulgaris) Hairy Roots in a Bubble Column Reactor

2007 ◽  
Vol 62 (5-6) ◽  
pp. 439-446 ◽  
Author(s):  
Atanas Pavlov ◽  
Milen Georgiev ◽  
Thomas Bley
Keyword(s):  
Beta Vulgaris ◽  
Hairy Roots ◽  
Bubble Column ◽  
Dry Weight ◽  
Bubble Column Reactor ◽  
Fed Batch ◽  
Column Reactor ◽  
Red Beet ◽  
Air Supply ◽  
Dry Biomass

Hairy root cultures from red beet (Beta vulgaris L.), which could be used for the commercial production of biologically active betalain pigments, were cultivated in a 3 L bubble column bioreactor in batch mode with various rates of air supply. Both the growth of the roots and betalain volumetric yields were highest (12.7 g accumulated dry biomass/L and 330.5 mg/ L, respectively) with a 10 L/h (0.083 vvm) air supply. The air flow rate also influenced the betacyanins/betaxanthins ratios in the cultures. Growth and betalains production were then examined in two fed-batch regimes (with a 10 L/h air supply), in which nutrient medium was fed just once or on five occasions, designated FBI and FBII, respectively. The root mass accumulation was increased in the FBI feeding regime (to 13.3 g accumulated dry biomass/ L), while in FBII the betalains content was ca. 11% higher (15.1 mg betacyanins/g dry weight and 14.0 mg betaxanthins/g dry weight) than in the most productive batch regime. Data on the time course of the utilization of major components in the medium during both operational modes were also collected. The implications of the information acquired are discussed, and the performance of the hairy roots (in terms of both growth and betalains production) in the bubble column reactor and previously investigated cultivation systems is compared.

Transient large-scale two-phase flow structures in a 3D bubble column reactor

2020 ◽  
Vol 127 ◽  
pp. 103236
Author(s):  
Mojtaba Goraki Fard ◽  
Anton Vernet ◽  
Youssef Stiriba ◽  
Xavier Grau
Keyword(s):  
Large Scale ◽  
Two Phase Flow ◽  
Bubble Column ◽  
Flow Structures ◽  
Bubble Column Reactor ◽  
Phase Flow ◽  
Two Phase ◽  
Column Reactor
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