Synthesis of antiparallel 4?-helix bundle TASP by chemoselective ligation

1994 ◽  
Vol 1 (1) ◽  
pp. 9-16 ◽  
Author(s):  
Orig�ne Nyanguile ◽  
Manfred Mutter ◽  
Gabriele Tuchscherer
Keyword(s):  
Chemoselective Ligation ◽  
Helix Bundle

Fmoc Solid Phase Peptide Synthesis

1999 ◽  
Keyword(s):  
Peptide Synthesis ◽  
Solid Phase ◽  
Solid Phase Peptide Synthesis ◽  
Peptide Chain ◽  
Standard Approach ◽  
Side Chain ◽  
Complex Structures ◽  
Protein Conjugation ◽  
Chemoselective Ligation ◽  
Routine Production

In the years since the publication of Atherton and Sheppard's volume, the technique of Fmoc solid-phase peptide synthesis has matured considerably and is now the standard approach for the routine production of peptides. The basic problems outstanding at the time of publication of this earlier work have now been, for the most part, solved. As a result, innovators in the field have focussed their efforts to develop methodologies and chemistry for the synthesis of more complex structures. The focus of this new volume is much broader, and covers not only the essential procedures for the production of linear peptides but also more advanced techniques for preparing cyclic, side-chain modified, phospho- and glycopeptides. Many other methods also deserving attention have been included: convergent peptide synthesis; peptide-protein conjugation; chemoselective ligation; and chemoselective purification. The difficult preparation of cysteine and methionine-containing peptides is also covered, as well as methods for overcoming aggregation during peptide chain assembly and a survey of available automated instrumentation.

Chemoselective ligation assisted DNA walker for analysis of double targets

2021 ◽  
Vol 201 ◽  
pp. 111620
Author(s):  
Tianxiang Xue ◽  
Longfei Tang ◽  
Xiquan Yue ◽  
Lili Niu ◽  
Jinhui Peng ◽  
...  
Keyword(s):  
Chemoselective Ligation ◽  
Dna Walker ◽  
Double Targets

scholarly journals Rational thermostabilisation of four-helix bundle dimeric de novo proteins

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Shin Irumagawa ◽  
Kaito Kobayashi ◽  
Yutaka Saito ◽  
Takeshi Miyata ◽  
Mitsuo Umetsu ◽  
...  
Keyword(s):  
Protein Design ◽  
De Novo ◽  
Protein Complexes ◽  
Salt Bridge ◽  
Dynamics Simulation ◽  
Saturation Mutagenesis ◽  
Helix Bundle ◽  
Stable Mutant ◽  
Four Helix Bundle ◽  
The Stability

AbstractThe stability of proteins is an important factor for industrial and medical applications. Improving protein stability is one of the main subjects in protein engineering. In a previous study, we improved the stability of a four-helix bundle dimeric de novo protein (WA20) by five mutations. The stabilised mutant (H26L/G28S/N34L/V71L/E78L, SUWA) showed an extremely high denaturation midpoint temperature (Tm). Although SUWA is a remarkably hyperstable protein, in protein design and engineering, it is an attractive challenge to rationally explore more stable mutants. In this study, we predicted stabilising mutations of WA20 by in silico saturation mutagenesis and molecular dynamics simulation, and experimentally confirmed three stabilising mutations of WA20 (N22A, N22E, and H86K). The stability of a double mutant (N22A/H86K, rationally optimised WA20, ROWA) was greatly improved compared with WA20 (ΔTm = 10.6 °C). The model structures suggested that N22A enhances the stability of the α-helices and N22E and H86K contribute to salt-bridge formation for protein stabilisation. These mutations were also added to SUWA and improved its Tm. Remarkably, the most stable mutant of SUWA (N22E/H86K, rationally optimised SUWA, ROSA) showed the highest Tm (129.0 °C). These new thermostable mutants will be useful as a component of protein nanobuilding blocks to construct supramolecular protein complexes.

Highly chemoselective ligation of thiol- and amino-peptides on a bromomaleimide core

2016 ◽  
Vol 52 (11) ◽  
pp. 2334-2337 ◽  
Author(s):  
Suhas Ramesh ◽  
Prabhakar Cherkupally ◽  
Thavendran Govender ◽  
Hendrik G. Kruger ◽  
Fernando Albericio ◽  
...  
Keyword(s):  
Chemoselective Ligation ◽  
P Application

Application of a bromomaleimide core allows for the incorporation of three different peptides.

De NovoDesign and Synthesis of Four α-Helix Bundle Proteins with Flavin Function

1993 ◽  
Vol 22 (9) ◽  
pp. 1533-1536 ◽  
Author(s):  
Hisakazu Mihara ◽  
Kin-ya Tomizaki ◽  
Norikazu Nishino ◽  
Tsutomu Fujimoto
Keyword(s):  
Helix Bundle ◽  
Α Helix

Self-Assembly of a Four-Helix Bundle on a DNA Quadruplex

2009 ◽  
Vol 48 (15) ◽  
pp. 2749-2751 ◽  
Author(s):  
Brooke A. Rosenzweig ◽  
Andrew D. Hamilton
Keyword(s):  
Self Assembly ◽  
Helix Bundle ◽  
Dna Quadruplex ◽  
Four Helix Bundle
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