Ethanol production by zymomonas mobilis: Effect of temperature on cell growth, ethanol production and intracellular ethanol accumulation

1982 ◽  
Vol 4 (8) ◽  
pp. 537-542 ◽  
Author(s):  
I. Laudrin ◽  
G. Goma
Keyword(s):  
Cell Growth ◽  
Ethanol Production ◽  
Zymomonas Mobilis ◽  
Effect Of Temperature

The effect of temperature on the kinetics of ethanol production by strains of Zymomonas mobilis

1981 ◽  
Vol 3 (6) ◽  
pp. 291-296 ◽  
Author(s):  
K. J. Lee ◽  
M. L. Skotnicki ◽  
D. E. Tribe ◽  
P. L. Rogers
Keyword(s):  
Ethanol Production ◽  
Zymomonas Mobilis ◽  
Effect Of Temperature ◽  
Kinetics Of

scholarly journals Investigation of the impact of a broad range of temperatures on the physiological and transcriptional profiles of Zymomonas mobilis ZM4 for high-temperature-tolerant recombinant strain development

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Runxia Li ◽  
Wei Shen ◽  
Yongfu Yang ◽  
Jun Du ◽  
Mian Li ◽  
...  
Keyword(s):  
High Temperature ◽  
Ethanol Production ◽  
Heat Tolerance ◽  
Zymomonas Mobilis ◽  
Glucose Utilization ◽  
Effect Of Temperature ◽  
Rna Seq ◽  
Transcriptional Profiles ◽  
Different Temperatures ◽  
The Impact

AbstractThe model ethanologenic bacterium Zymomonas mobilis has many advantages for diverse biochemical production. Although the impact of temperature especially high temperature on the growth and ethanol production of Z. mobilis has been reported, the transcriptional profiles of Z. mobilis grown at different temperatures have not been systematically investigated. In this study, Z. mobilis wild-type strain ZM4 was used to study the effect of a broad range of temperatures of 24, 30, 36, 40, and 45 °C on cell growth and morphology, glucose utilization and ethanol production, as well as the corresponding global gene expression profiles using RNA-Seq-based transcriptomics. In addition, a recombinant Z. mobilis strain expressing reporter gene EGFP (ZM4_EGFP) was constructed to study the effect of temperature on heterologous protein expression at different temperatures. Our result demonstrated that the effect of temperature on the growth and morphology of ZM4 and ZM4_EGFP were similar. The biomass of these two strains decreased along with the temperature increase, and an optimal temperature range is needed for efficient glucose utilization and ethanol production. Temperatures lower or higher than normal temperature investigated in this work was not favorable for the glucose utilization and ethanol production as well as the expression of exogenous protein EGFP based on the results of flow cytometry and Western blot. Temperature also affected the transcriptional profiles of Z. mobilis especially under high temperature. Compared with ZM4 cultured at 30 °C, 478 genes were up-regulated and 481 genes were down-regulated at 45 °C. The number of differentially expressed genes of ZM4 cultured at other temperatures (24, 36 or 40 °C) was relatively small though compared with those at 30 °C. Since temperature usually increases during the fermentation process, and heat tolerance is one of the important robustness traits of industrial strains, candidate genes related to heat resistance based on our RNA-Seq result and literature report were then selected for genetics study using the strategies of plasmid overexpression of candidate gene or replacement of the native promoter of candidate gene by an inducible Ptet promoter. The genetics studies indicated that ZMO0236, ZMO1335, ZMO0994, operon groESL, and cspL, which encodes Mrp family chromosome partitioning ATPase, flavoprotein WrbA, an uncharacterized protein, chaperonin Cpn10 and GroEL, and an exogenous cold shock protein, respectively, were associated with heat tolerance, and recombinant strains over-expressing these genes can improve their heat tolerance. Our work thus not only explored the effects of temperature on the expression of exogenous gene EGFP and endogenous genes, but also selected and confirmed several genes associated with heat tolerance in Z. mobilis, which provided a guidance on identifying candidate genes associated with phenotypic improvement through systems biology strategy and genetics studies for other microorganisms.

scholarly journals Sorbitol required for cell growth and ethanol production by Zymomonas mobilis under heat, ethanol, and osmotic stresses

2013 ◽  
Vol 6 (1) ◽  
pp. 180 ◽  
Author(s):  
Kaewta Sootsuwan ◽  
Pornthap Thanonkeo ◽  
Nawapote Keeratirakha ◽  
Sudarat Thanonkeo ◽  
Prasit Jaisil ◽  
...  
Keyword(s):  
Cell Growth ◽  
Ethanol Production ◽  
Zymomonas Mobilis

The influence of the initial concentrations of glucose and yeast extract on the ethanolic fermentation by Pachysolen tannophilus

1990 ◽  
Vol 55 (3) ◽  
pp. 854-866 ◽  
Author(s):  
Rodríguez V. Bravo ◽  
Rubio F. Camacho ◽  
Villasclaras S. Sánchez ◽  
Vico M. Castro
Keyword(s):  
Cell Growth ◽  
Ethanol Production ◽  
Yeast Extract ◽  
Culture Medium ◽  
Ethanolic Fermentation ◽  
Pachysolen Tannophilus ◽  
Significant Component ◽  
Batch Cultures

The ethanolic fermentation in batch cultures of Pachysolen tannophilus was studied experimentally varying the initial concentrations of two of the components in the culture medium: glucose between 0 and 200 g l-1 and yeast extract between 0 and 8 g l-1. The yeast extract appears to be a significant component both in cell growth and for ethanol production.

scholarly journals The Quorum Sensing Auto-Inducer 2 (AI-2) Stimulates Nitrogen Fixation and Favors Ethanol Production over Biomass Accumulation in Zymomonas mobilis

2021 ◽  
Vol 22 (11) ◽  
pp. 5628
Author(s):  
Valquíria Campos Alencar ◽  
Juliana de Fátima dos Santos Silva ◽  
Renata Ozelami Vilas Boas ◽  
Vinícius Manganaro Farnézio ◽  
Yara N. L. F. de Maria ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Quorum Sensing ◽  
Ethanol Production ◽  
N2 Fixation ◽  
Energy Demand ◽  
Zymomonas Mobilis ◽  
Biomass Accumulation ◽  
High Energy ◽  
Gram Negative ◽  
Expression Of Genes

Autoinducer 2 (or AI-2) is one of the molecules used by bacteria to trigger the Quorum Sensing (QS) response, which activates expression of genes involved in a series of alternative mechanisms, when cells reach high population densities (including bioluminescence, motility, biofilm formation, stress resistance, and production of public goods, or pathogenicity factors, among others). Contrary to most autoinducers, AI-2 can induce QS responses in both Gram-negative and Gram-positive bacteria, and has been suggested to constitute a trans-specific system of bacterial communication, capable of affecting even bacteria that cannot produce this autoinducer. In this work, we demonstrate that the ethanologenic Gram-negative bacterium Zymomonas mobilis (a non-AI-2 producer) responds to exogenous AI-2 by modulating expression of genes involved in mechanisms typically associated with QS in other bacteria, such as motility, DNA repair, and nitrogen fixation. Interestingly, the metabolism of AI-2-induced Z. mobilis cells seems to favor ethanol production over biomass accumulation, probably as an adaptation to the high-energy demand of N2 fixation. This opens the possibility of employing AI-2 during the industrial production of second-generation ethanol, as a way to boost N2 fixation by these bacteria, which could reduce costs associated with the use of nitrogen-based fertilizers, without compromising ethanol production in industrial plants.

scholarly journals Ethanol Production from Glucose and Xylose by Immobilized Zymomonas mobilis CP4(pZB5)

2000 ◽  
Vol 84-86 (1-9) ◽  
pp. 525-542 ◽  
Author(s):  
Mahesh S. Krishnan ◽  
Maria Blanco ◽  
Christopher K. Shattuck ◽  
Nhuan P. Nghiem ◽  
Brian H. Davison
Keyword(s):  
Ethanol Production ◽  
Zymomonas Mobilis

scholarly journals Ethanol production from Jerusalem artichoke (Helianthus tuberosus L.) by Zymomonas mobilis TISTR548

2011 ◽  
Vol 10 (52) ◽  
pp. 10691-10697 ◽  
Author(s):  
Thanonkeo Pornthap ◽  
Thanonkeo Sudarat ◽  
Charoensuk Kannikar ◽  
Yamada Mamoru
Keyword(s):  
Ethanol Production ◽  
Zymomonas Mobilis ◽  
Jerusalem Artichoke ◽  
Helianthus Tuberosus
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