Neural cells in culture: models for the purification and the study of the effects of growth factors

1992 ◽  
Vol 8 (3) ◽  
pp. 107-115
Author(s):  
Brigitte Pettmann ◽  
Marston Manthorpe
Keyword(s):  
Growth Factors ◽  
Neural Cells ◽  
Cells In Culture ◽  
Culture Models

T cells show preferential adhesion to enteric neural cells in culture and are close to neural cells in the myenteric ganglia of Crohn's patients

2020 ◽  
Vol 349 ◽  
pp. 577422
Author(s):  
Julie Pabois ◽  
Tony Durand ◽  
Catherine Le Berre ◽  
Jacques Gonzales ◽  
Michel Neunlist ◽  
...  
Keyword(s):  
T Cells ◽  
Neural Cells ◽  
Cells In Culture ◽  
Myenteric Ganglia

Effects of oestrogen on human breast cancer cells in culture

1989 ◽  
Vol 95 ◽  
pp. 119-132 ◽  
Author(s):  
Philippa D. Darbre ◽  
Roger J. Daly
Keyword(s):  
Breast Cancer ◽  
Growth Factors ◽  
Cell Lines ◽  
Human Breast Cancer ◽  
Breast Cancer Cell Lines ◽  
Phenol Red ◽  
Human Breast ◽  
Cells In Culture ◽  
D Cells

SynopsisOestrogen regulates the growth of human breast cancer cell lines ZR-75–1, T-47-D and MCF-7 (KO and McGrath). Basal cell growth can be reduced (T-47-D) or eliminated (ZR-75–1) by prior growth in the absence of steroid and phenol red for three weeks, demonstrating that oestrogens can have long-lasting effects on cells in culture (termed “steroid memory”). Effects of oestradiol on different cell biological parameters are described and interaction with other steroids and serum growth factors is discussed. Antioestrogen action in these cell lines is affected by at least five parameters: (1) presence of phenol red, (2) time in culture, (3) cell density, (4) antioestrogen concentration, (5) steroid memory.An in vitro model for loss of oestrogen sensitivity in breast cancer is presented. Both dependent (ZR-75–1) and responsive (T-47-D) cells lose oestrogen sensitivity when deprived of steroid in the long term but show a gradual increase in growth. For ZR-75–1 cells, the effects appear to be clonal but occur at a high frequency (about 1 in 1,000 cells). Parallel alterations in sensitivity to other steroids, antioestrogens and serum growth factors are shown. Molecular markers of this action are described and the results compared with the well-established model for loss of androgen/glucocorticoid sensitivity in SI 15 cells.

Secretion of Polypeptide Growth Factors by Human Nonfunctioning Pituitary Adenoma Cells in Culture

1993 ◽  
Vol 57 (5) ◽  
pp. 825-834 ◽  
Author(s):  
Ulrich Renner ◽  
Juri Mojto ◽  
Eduardo Arzt ◽  
Manfred Lange ◽  
Johanna Stalla ◽  
...  
Keyword(s):  
Growth Factors ◽  
Pituitary Adenoma ◽  
Nonfunctioning Pituitary Adenoma ◽  
Cells In Culture ◽  
Polypeptide Growth Factors
Sign in / Sign up

Export Citation Format

Share Document