Nucleotide sequence and transcriptional studies of the vaccinia virusKpnI I DNA fragment

Virus Genes ◽  
1989 ◽  
Vol 3 (2) ◽  
Author(s):  
LeslieA. Tengelsen ◽  
DennisE. Hruby
Keyword(s):  
Nucleotide Sequence ◽  
Dna Fragment

scholarly journals Molecular analysis of enterolysin a and entL gene cluster from natural isolate Enterococcus faecalis BGPT1-10P

Genetika ◽  
2013 ◽  
Vol 45 (2) ◽  
pp. 479-492 ◽  
Author(s):  
Katarina Veljovic ◽  
Amarela Terzic-Vidojevic ◽  
Maja Tolinacki ◽  
Milan Kojic ◽  
Ljubisa Topisirovic
Keyword(s):  
Functional Analysis ◽  
Nucleotide Sequence ◽  
Enterococcus Faecalis ◽  
Broad Spectrum ◽  
Candida Species ◽  
Genetic Information ◽  
Natural Isolate ◽  
Gene Encoding ◽  
Dna Fragment ◽  
Virulent Factor

Strain Enterococcus faecalis BGPT1-10P was isolated from artisanal semi-hard homemade cheese from Stara Planina, Serbia. Results showed that BGPT1-10P synthesized a heat labile bacteriocin with a broad spectrum of activity, including Listeria and Candida species. Further analysis revealed that synthesized bacteriocin is enterolysin A. Moreover, the entL gene encoding enterolysin A was found to be located on the chromosome. The entL gene was cloned and sequenced. Analysis of nucleotide sequence showed that the entL gene in natural isolate En. faecalis BGPT1-10P is identical to that of the entL gene described previously in En. faecalis LMG 2333. Within the cloned DNA fragment containing the entL gene, four ORFs were detected. One of them was identified as the scpE gene, which encodes a virulent factor staphopain peptidase. Functional analysis of the entL gene showed that the complete genetic information necessary for the synthesis of enterolysin A were directly linked solely to it. Strain BGPT1-10P also synthesized gelatinase and citolysin, and contained a set of virulent factors. In addition, BGPT1-10P carries the ermB and tetM genes conferring the resistance to erythromycin and tetracycline, respectively.

Nucleotide Sequence of a Cloned Murine Leukemia Virus DNA Fragment

1980 ◽  
Vol 44 (0) ◽  
pp. 1275-1279
Author(s):  
J. G. Sutcliffe ◽  
T. M. Shinnick ◽  
R. A. Lerner ◽  
P. Johnson ◽  
I. M. Verma
Keyword(s):  
Nucleotide Sequence ◽  
Murine Leukemia Virus ◽  
Leukemia Virus ◽  
Dna Fragment ◽  
Murine Leukemia

Nucleotide sequence of a DNA fragment that contains the abi gene of the ColIb plasmid

Plasmid ◽  
1988 ◽  
Vol 20 (3) ◽  
pp. 194-206 ◽  
Author(s):  
S.K. Gupta ◽  
D.J. McCorquodale
Keyword(s):  
Nucleotide Sequence ◽  
Dna Fragment

scholarly journals Nucleotide sequence of adenovirus 2 DNA fragment encoding for the carboxylic region of the fiber protein and the entire E4 region

1981 ◽  
Vol 9 (16) ◽  
pp. 4023-4042 ◽  
Author(s):  
J. Hérissé ◽  
M. Rigolet ◽  
S. Dupont de Dinechin ◽  
F. Galibert
Keyword(s):  
Nucleotide Sequence ◽  
E4 Region ◽  
Fiber Protein ◽  
Dna Fragment

scholarly journals The TRI11 Gene of Fusarium sporotrichioides Encodes a Cytochrome P-450 Monooxygenase Required for C-15 Hydroxylation in Trichothecene Biosynthesis

1998 ◽  
Vol 64 (1) ◽  
pp. 221-225 ◽  
Author(s):  
Nancy J. Alexander ◽  
Thomas M. Hohn ◽  
Susan P. McCormick
Keyword(s):  
Sequence Analysis ◽  
Nucleotide Sequence ◽  
Gene Family ◽  
Gene Cluster ◽  
Biosynthetic Pathway ◽  
Fusarium Sporotrichioides ◽  
Significant Similarity ◽  
Dna Fragment ◽  
Cytochrome P 450 ◽  
Cluster Sequence

ABSTRACT Several genes in the trichothecene biosynthetic pathway ofFusarium sporotrichioides have been shown to reside in a gene cluster. Sequence analysis of a cloned DNA fragment located 3.8 kb downstream from TRI5 has led to the identification of the TRI11 gene. The nucleotide sequence ofTRI11 predicts a polypeptide of 492 residues (M r = 55,579) with significant similarity to members of the cytochrome P-450 superfamily. TRI11 is most similar to several fungal cytochromes P-450 (23 to 27% identity) but is sufficiently distinct to define a new cytochrome P-450 gene family, designated CYP65A1. Disruption of TRI11 results in an altered trichothecene production phenotype characterized by the accumulation of isotrichodermin, a trichothecene pathway intermediate. The evidence suggests that TRI11 encodes a C-15 hydroxylase involved in trichothecene biosynthesis.

scholarly journals Characterization of the Type 8 Capsular Gene Cluster of Streptococcus pneumoniae

1999 ◽  
Vol 181 (19) ◽  
pp. 6214-6219 ◽  
Author(s):  
Rosario Muñoz ◽  
Marta Mollerach ◽  
Rubens López ◽  
Ernesto García
Keyword(s):  
Streptococcus Pneumoniae ◽  
Nucleotide Sequence ◽  
Gene Cluster ◽  
Complete Nucleotide Sequence ◽  
Capsular Polysaccharide ◽  
Open Reading Frames ◽  
Dna Fragment ◽  
Type 3 ◽  
Reading Frames

ABSTRACT The complete nucleotide sequence of the capsular gene cluster (cap8) responsible for the biosynthesis of the capsular polysaccharide of Streptococcus pneumoniae type 8 has been determined. The cap8 gene cluster, located between the genes dexB and aliA, is composed of 12 open reading frames. A 14.7-kb DNA fragment embracing the cap8genes was sufficient to transform an unencapsulated type 3 S. pneumoniae strain to a strain with the type 8 capsule. A possible scenario for the evolution of pneumococcal types 2 and 8 is outlined.

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