Role of extracellular matrix proteins in regulation of human glioma cell invasion in vitro

1996 ◽  
Vol 14 (4) ◽  
pp. 358-366 ◽  
Author(s):  
Shravan K. Chintala ◽  
Ziya L. Gokaslan ◽  
Yoshinori Go ◽  
Raymond Sawaya ◽  
Garth L. Nicolson ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Cell Invasion ◽  
Glioma Cell ◽  
Extracellular Matrix Proteins ◽  
Matrix Proteins ◽  
Human Glioma ◽  
Human Glioma Cell

Organization of F-actin filaments in human glioma cell lines cultured on extracellular matrix proteins

1996 ◽  
Vol 27 (3) ◽  
pp. 215-224 ◽  
Author(s):  
Nobuhisa Nagano ◽  
Masaru Aoyagi ◽  
Kimiyoshi Hirakawa ◽  
Mari Yamamoto ◽  
Kiyotaka Yamamoto
Keyword(s):  
Extracellular Matrix ◽  
Cell Lines ◽  
Actin Filaments ◽  
Glioma Cell ◽  
Extracellular Matrix Proteins ◽  
Matrix Proteins ◽  
Human Glioma ◽  
Human Glioma Cell ◽  
Glioma Cell Lines

Role of high molecular weight extracellular matrix proteins in glioma cell migration

1997 ◽  
Vol 23 (2) ◽  
pp. 102-112 ◽  
Author(s):  
R. Mahesparan ◽  
B. B. Tysnes ◽  
K. Edvardsen ◽  
H. K. Haugeland ◽  
I. Garcia Cabrera ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Extracellular Matrix ◽  
Cell Migration ◽  
High Molecular Weight ◽  
Glioma Cell ◽  
Extracellular Matrix Proteins ◽  
Matrix Proteins ◽  
Glioma Cell Migration

scholarly journals High-Density Lipoproteins Limit Neutrophil-Induced Damage to the Blood–Brain Barrier in Vitro

2013 ◽  
Vol 33 (4) ◽  
pp. 575-582 ◽  
Author(s):  
Quoc Bao Dang ◽  
Bertrand Lapergue ◽  
Alexy Tran-Dinh ◽  
Devy Diallo ◽  
Juan-Antonio Moreno ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Blood Brain Barrier ◽  
Extracellular Matrix Proteins ◽  
High Density ◽  
Matrix Proteins ◽  
Brain Barrier ◽  
High Density Lipoproteins ◽  
Blood Brain

Breakdown of the blood–brain barrier (BBB) is a key step associated with ischemic stroke and its increased permeability causes extravasation of plasma proteins and circulating leukocytes. Polymorphonuclear neutrophil (PMN) proteases may participate in BBB breakdown. We investigated the role of PMNs in ischemic conditions by testing their effects on a model of BBB in vitro, under oxygen-glucose deprivation (OGD) to mimic ischemia, supplemented or not with high-density lipoproteins (HDLs) to assess their potential protective effects. Human cerebral endothelial cells cultured on transwells were incubated for 4 hours under OGD conditions with or without PMNs and supplemented or not with HDLs or alpha-1 antitrypsin (AAT, an elastase inhibitor). The integrity of the BBB was then assessed and the effect of HDLs on PMN-induced proteolysis of extracellular matrix proteins was evaluated. The release of myeloperoxidase and matrix metalloproteinase 9 (MMP-9) by PMNs was quantified. Polymorphonuclear neutrophils significantly increased BBB permeability under OGD conditions via proteolysis of extracellular matrix proteins. This was associated with PMN degranulation. Addition of HDLs or AAT limited the proteolysis and associated increased permeability by inhibiting PMN activation. Our results suggest a deleterious, elastase-mediated role of activated PMNs under OGD conditions leading to BBB disruption that could be inhibited by HDLs.

Immunohistochemical localization of extracellular matrix proteins in human glioma, both in vivo and in vitro

1996 ◽  
Vol 101 (1) ◽  
pp. 107-114 ◽  
Author(s):  
Shravan Kumar Chintala ◽  
Raymond Sawaya ◽  
Ziya Levent Gokaslan ◽  
Gregory Fuller ◽  
Jasti Sambasiva Rao
Keyword(s):  
Extracellular Matrix ◽  
Extracellular Matrix Proteins ◽  
Immunohistochemical Localization ◽  
Matrix Proteins ◽  
Human Glioma

scholarly journals Functional Effect of Contortrostatin, a Snake Venom Disintegrin, on Human Glioma Cell Invasion In Vitro

2003 ◽  
Vol 10 (1) ◽  
pp. 1-16 ◽  
Author(s):  
Stephanie Schmitmeier ◽  
Francis S. Markland ◽  
Matthew R. Ritter ◽  
David E. Sawcer ◽  
Thomas C. Chen
Keyword(s):  
Snake Venom ◽  
Cell Invasion ◽  
Glioma Cell ◽  
Human Glioma ◽  
Functional Effect ◽  
Human Glioma Cell

The Role of Integrin αDβ2 in Macrophage Migration.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 2215-2215
Author(s):  
Valentin P. Yakubenko ◽  
Tatiana P. Ugarova
Keyword(s):  
Extracellular Matrix ◽  
Cell Migration ◽  
Extracellular Matrix Proteins ◽  
In Vitro Studies ◽  
Matrix Proteins ◽  
Hek293 Cells ◽  
Macrophage Migration ◽  
Blocking Antibodies

Abstract Integrin αDβ2 (CD11d/CD18), the most recently discovered member of the β2 sub-family of adhesion receptors, is strongly upregulated on macrophage foam cells which underscores its potential role in atherosclerosis. However, the contribution of αDβ2 to monocyte/macrophage adhesive reactions and the significance of its overexpression on these cells remain unknown. Recently we characterized αDβ2 as a multiligand receptor capable of binding many extracellular matrix proteins with the recognition specificity overlapping that of the major myeloid-specific integrin αMβ2 (Mac-1). We hypothesized that the αDβ2 ability to bind numerous ligands in the extracellular matrix and its capacity to be upregulated to high density on the surface of macrophages may modulate cell adhesiveness and, thus, affect migration. To evaluate the role of αDβ2 in migration, we generated model and natural cells expressing different densities of αDβ2 and tested their migration to different extracellular matrix proteins. In vitro studies demonstrated that αDβ2 expressed at low densities, either on the surface of HEK293 cells or the mouse macrophage cell line IC-21, supported migration which was partially inhibited by anti-αD function-blocking antibodies. Furthermore, β1 and β3 integrins expressed on HEK293 cells and IC-21 macrophages, respectively, contributed to migration because anti-β1 and anti-β3 antibodies inhibited migration. Increased expression of αDβ2 on the surface of HEK293 cells and its upregulation by PMA on IC-21 macrophages resulted in the inhibition of cell migration. Ligation of αDβ2 with anti-αD antibodies restored β1- and β3-driven cell migration by means of removing restraints imposed by the excess of the αDβ2-ligand adhesive bonds. To test the possibility that progressive upregulation of αDβ2 can block macrophage migration in vivo, we assessed the effect of anti-αD function blocking antibodies using the thioglycollate-induced peritonitis model. More than 4-fold upregulation of αDβ2 was detected on macrophages in 72 h after thioglycollate stimulation and, similar to in vitro studies, the numbers of migration macrophages increased in the presence of anti-αD antibodies. These results demonstrate that the density of αDβ2 can modulate cell migration and suggest that low levels of αDβ2 can contribute to monocyte migration while αDβ2 upregulation on differentiated macrophages might facilitate their retention at the site of inflammation.

Role of high molecular weight extracellular matrix proteins in glioma cell migration

1997 ◽  
Vol 23 (3) ◽  
pp. 102-112 ◽  
Author(s):  
R. Mahesparan ◽  
B. B. Tysnes ◽  
K. Edvardsen ◽  
H. K. Haugeland ◽  
I. Garcia Cabrera ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Extracellular Matrix ◽  
Cell Migration ◽  
High Molecular Weight ◽  
Glioma Cell ◽  
Extracellular Matrix Proteins ◽  
Matrix Proteins ◽  
Glioma Cell Migration

Cell surface gangliosides are involved in the control of human glioma cell invasion in vitro

1994 ◽  
Vol 177 (1-2) ◽  
pp. 44-46 ◽  
Author(s):  
Abderrahim Merzak ◽  
Shahriar Koochekpour ◽  
Geoffrey J Pilkington
Keyword(s):  
Cell Surface ◽  
Cell Invasion ◽  
Glioma Cell ◽  
Human Glioma ◽  
Human Glioma Cell
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