The genetic basis of pyrethroid and DDT resistance inter-relationships in Aedes aegypti I. Isolation of DDT and pyrethroid resistance factors

Genetica ◽  
1983 ◽  
Vol 60 (3) ◽  
pp. 213-219 ◽  
Author(s):  
C. A. Malcolm
Keyword(s):  
Aedes Aegypti ◽  
Genetic Basis ◽  
Pyrethroid Resistance ◽  
Resistance Factors ◽  
Ddt Resistance

Insecticide Resistance Patterns and Mechanisms in Aedes aegypti (Diptera: Culicidae) Populations Across Abidjan, Côte d’Ivoire Reveal Emergent Pyrethroid Resistance

2021 ◽  
Author(s):  
Lucien Yao Konan ◽  
Welbeck Achille Oumbouke ◽  
Urbain Garhapié Silué ◽  
Ibrahima Zanakoungo Coulibaly ◽  
Jean-Claude Tokou Ziogba ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Cote D'ivoire ◽  
Côte D’Ivoire ◽  
Pyrethroid Resistance ◽  
Population Based ◽  
Cote D’Ivoire ◽  
Low Frequencies ◽  
Larval Habitats ◽  
Côte D'ivoire ◽  
The City

Abstract From 2008 to 2017, the city of Abidjan, Côte d’Ivoire experienced several Aedes-borne disease epidemics which required control of the vector mosquito population based on the reduction of larval habitats and insecticidal sprays for adult mosquitoes. This study was undertaken to assess the insecticide susceptibility status of Aedes aegypti (Linnaeus) in the city of Abidjan. Immature Ae. aegypti were sampled from several larval habitats within seven communes of Abidjan and reared to adults. Three to five days old F1 emerged adults were tested for susceptibility using insecticide-impregnated papers and the synergist piperonyl butoxide (PBO) following WHO bioassay guidelines. The results showed that Ae. aegypti populations from Abidjan were resistant to 0.1% propoxur, and 1% fenitrothion, with mortality rates ranging from 0% to 54.2%. Reduced susceptibility (93.4–97.5% mortality) was observed to 0.05% deltamethrin, 0.75% permethrin, 0.05% lambda-cyhalothrin, 5% malathion, and 0.8% chlorpyrifos-methyl. This reduced susceptibility varied depending on the insecticide and the collection site. The restoration of mortality when the mosquitoes were pre-exposed to the synergist PBO suggests that increased activity of oxidases could be contributing to resistance. Three kdr mutations (V410L, V1016I, and F1534C) were present in populations tested, with low frequencies for the Leu410 (0.28) and Ile1016 (0.32) alleles and high frequencies for the Cys1534 allele (0.96). These findings will be used to inform future arbovirus vector control activities in Abidjan.

Dehydrochlorination and DDT-Resistance in Aedes aegypti

1963 ◽  
Vol 56 (4) ◽  
pp. 511-517 ◽  
Author(s):  
Z. H. Abedi ◽  
J. R. Duffy ◽  
A. W. A. Brown
Keyword(s):  
Aedes Aegypti ◽  
Ddt Resistance

Biochemical and molecular analyses to determine pyrethroid resistance in Aedes aegypti

2013 ◽  
Vol 107 (2) ◽  
pp. 266-276 ◽  
Author(s):  
Ying-Hsi Lin ◽  
Wei-Lun Tsen ◽  
Nai-Yueh Tien ◽  
Yi-Pey Luo
Keyword(s):  
Aedes Aegypti ◽  
Pyrethroid Resistance ◽  
Molecular Analyses

scholarly journals Failure of Permethrin-Treated Military Uniforms to Protect Against a Laboratory-Maintained Knockdown-Resistant Strain of Aedes aegypti

2020 ◽  
Vol 36 (2) ◽  
pp. 127-130
Author(s):  
Alden S. Estep ◽  
Neil D. Sanscrainte ◽  
Ingeborg Cuba ◽  
Gregory M. Allen ◽  
James J. Becnel ◽  
...  
Keyword(s):  
Puerto Rico ◽  
Aedes Aegypti ◽  
Resistant Strain ◽  
Untreated Control ◽  
Pyrethroid Resistance ◽  
Disease Vectors ◽  
Significant Protection ◽  
Additional Testing ◽  
Resistant Strains ◽  
Preliminary Study

ABSTRACT Military forces and the recreational industry rely on the repellent properties of permethrin-treated fabrics and N,N-diethyl-meta-toluamide (deet)–based lotions to provide protection from disease vectors and hematophagous organisms. Concerns regarding efficacy have been raised as pyrethroid resistance becomes more common and recent publications present contradictory conclusions. In this preliminary study, consenting volunteers were exposed to pyrethroid-susceptible and pyrethroid-resistant Aedes aegypti strains while wearing sleeves of untreated or permethrin-treated army uniform fabric as well as with untreated or deet-treated exposed forearms. Deet was nearly 100% effective against both susceptible and resistant strains. However, permethrin treatment provided no significant protection against the resistant Puerto Rico strain relative to untreated control sleeves. These results confirm that pyrethroid-resistant vectors can negate the efficacy of permethrin-treated uniforms. Additional testing with resistant field strains is needed to better understand the risk to service members.

Section 10. Pyrethroid resistance: resistance breaking pyrethroids

1993 ◽  
Vol 1 ◽  
pp. 83-96 ◽  
Author(s):  
Neil W. Forrester ◽  
Matthew Cahill ◽  
Lisa J. Bird ◽  
Jacquelyn K. Layland
Keyword(s):  
Resistant Strain ◽  
Pyrethroid Resistance ◽  
Residual Activity ◽  
Susceptible Strain ◽  
Piperonyl Butoxide ◽  
Monooxygenase System ◽  
Acid Moiety ◽  
Resistance Factors ◽  
Resistance Breaking ◽  
Alcohol Moiety

SummaryThe structural requirements for designing a resistance breaking pyrethroid to overcome oxidative metabolic pyrethroid resistance in Helicoverpa armigera were studied. A range of pyrethroid structures were tested on a well defined pure breeding pyrethroid resistant strain of H. armigera (homozygous for a metabolic detoxification mechanism fully suppressible by piperonyl butoxide, presumably via a microsomal monooxygenase system). Highest resistance factors were to the ester bonded phenoxybenzyl alcohol pyrethroids, particularly to those with an aromatic acid moiety. Changes to the alcohol moiety alone could overcome most, if not all, resistance. Simple benzyl alcohols were the most effective followed by cyclopentenolones and a methylated biphenyl alcohol. However, the benzylfurylmethyl alcohol (bioresmethrin) was not effective. The incorporation of a synergophore grouping into the alcohol moiety was fully effective for Scott's Py III (methylenedioxyphenyl) and prallethrin (propynyl) but only partially effective for tetramethrin (N-alkyl). Changes to the acid moiety had little effect except for the incorporation of a synergophore methylenedioxyphenyl grouping (Cheminova I) which was just as effective as for the same insertion in the alcohol moiety. The change to a central ether bond from the conventional ester bond lowered resistance. Reversion to an unsubstituted alpha carbon analogue from the conventional alpha cyano group also lowered resistance.Piperonyl butoxide (Pbo) had little effect on pyrethroid toxicity in the susceptible strain except for the single isomers deltamethrin and esfenvalerate. However, it was more than fully effective in overcoming resistance and actually reduced resistance factors to significantly below one in the resistant strain. This indicated the possibility that Pbo could be acting both as a classical monooxygenase inhibitor and a preferential penetration synergist in resistant larvae.Partial or full resolution of racemic mixtures had minimal impact on increasing toxicity in the susceptible strain. However, partially or fully resolved isomers were clearly much more toxic on resistant strains, indicating a possible blocking effect of the inactive isomers during the toxication process with the higher pyrethroid doses applied to resistant larvae. Cis isomers had only slightly higher resistance factors than trans isomers.Seven fully effective resistance breaking pyrethroids were identified in this study and one of these (the simple benzyl alcohol, Series Two) was shown to be equally effective on both adults and larvae of H. armigera. It was also shown to work equally well on laboratory or field material and gave results similar to a pyrethroid/Pbo combination. However, none of the resistance breakers identified so far are able to satisfy all of the requirements necessary for an ideal resistance breaking pyrethroid (i.e. good resistance breaking activity at low rates, photostability, residual activity similar to current pyrethroids and safety to mammals). Factors acting against the possible commercialization of successful resistance breaking compounds are discussed.

scholarly journals Vgsc-interacting proteins are genetically associated with pyrethroid resistance in Aedes aegypti

PLoS ONE ◽  
2019 ◽  
Vol 14 (1) ◽  
pp. e0211497 ◽  
Author(s):  
Corey L. Campbell ◽  
Karla Saavedra-Rodriguez ◽  
Tristan D. Kubik ◽  
Audrey Lenhart ◽  
Saul Lozano-Fuentes ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Pyrethroid Resistance ◽  
Interacting Proteins

scholarly journals Mechanisms of pyrethroid resistance inHaematobia irritans (Muscidae) from Mato Grosso do Sul state, Brazil

2013 ◽  
Vol 22 (1) ◽  
pp. 136-142 ◽  
Author(s):  
Antonio Thadeu Medeiros Barros ◽  
Teresinha Tizu Sato Schumaker ◽  
Wilson Werner Koller ◽  
Guilherme Marcondes Klafke ◽  
Thais Aguiar de Albuquerque ◽  
...  
Keyword(s):  
Pyrethroid Resistance ◽  
The State ◽  
Kdr Mutation ◽  
Low Frequencies ◽  
Mato Grosso ◽  
Pyrethroid Insecticides ◽  
Resistance Factors ◽  
Horn Fly ◽  
Polymerase Chain ◽  
Mato Grosso Do Sul

Horn fly resistance to pyrethroid insecticides occurs throughout Brazil, but knowledge about the involved mechanisms is still in an incipient stage. This survey was aimed to identify the mechanisms of horn fly resistance to cypermethrin in Mato Grosso do Sul state, Brazil. Impregnated filter paper bioassays using cypermethrin, synergized or not with piperonyl butoxide (PBO) and triphenyl phosphate (TPP), were conducted from March 2004 to June 2005 in horn fly populations (n = 33) from all over the state. All populations were highly resistant to cypermethrin, with resistance factors (RF) ranging from 89.4 to 1,020.6. Polymerase chain reaction (PCR) assays to detect the knockdown resistance (kdr) mutation also were performed in 16 samples. The kdr mutation was found in 75% of the tested populations, mostly with relatively low frequencies (<20%), and was absent in some highly resistant populations. Addition of TPP did not significantly reduce the LC50 in any population. However, PBO reduced LC50s above 40-fold in all tested populations, resulting in RFs ≤ 10 in most cases. Horn fly resistance to cypermethrin is widespread in the state, being primarily caused by an enhanced activity of P450 mono-oxygenases and secondarily by reduced target site sensitivity.

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