Effects of beta-blockers on HMG CoA reductase and LDL receptor activity in cultured human skin fibroblasts

1996 ◽  
Vol 10 (1) ◽  
pp. 67-74 ◽  
Author(s):  
Hiroshi Yoshida ◽  
Michio Suzukawa ◽  
Toshitsugu Ishikawa ◽  
Hideki Shige ◽  
Eisuke Nishio ◽  
...  
Keyword(s):  
Human Skin ◽  
Beta Blockers ◽  
Ldl Receptor ◽  
Skin Fibroblasts ◽  
Receptor Activity ◽  
Human Skin Fibroblasts ◽  
Hmg Coa Reductase ◽  
Coa Reductase

Cholesterol-mediated regulation of HMG-CoA reductase in microsomes from human skin fibroblasts and rat liver

1990 ◽  
Vol 68 (3) ◽  
pp. 674-679 ◽  
Author(s):  
R. George ◽  
P. J. Davis ◽  
L. Luong ◽  
M. J. Poznansky
Keyword(s):  
Tissue Culture ◽  
Enzyme Activity ◽  
Human Skin ◽  
Rat Liver ◽  
Reductase Activity ◽  
Cholesterol Content ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts ◽  
Hmg Coa Reductase ◽  
Coa Reductase

3-Hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase activity was determined in microsomes from human skin fibroblasts and rat liver that had been variously manipulated in vivo or in tissue culture to up- and down-regulate the enzyme. The cholesterol content of these microsomal preparations was then altered by depletion to or enrichment from either cholesterol-free or cholesterol-rich lipid vesicles. Microsomes from human skin fibroblasts responded to cholesterol depletion by increasing HMG-CoA reductase activity and by decreasing it in response to cholesterol enrichment. This was independent of the initial enzyme activity or the tissue culture conditions. Alterations in cholesterol content of rat liver microsomes in vitro failed to demonstrate any significant changes in HMG-CoA reductase activity whether the microsomes started with low enzyme activity (cholesterol-fed rats) or with high enzyme activity (cholestyramine-treated rats). The results are discussed in relation to previously published data and in respect to differences in the control of the human skin fibroblast and rat liver enzymes.Key words: cholesterol, HMG-CoA reductase, microsomes, fibroblasts, rat liver.

scholarly journals Effects of β-Blockers on 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase Activity in Cultured Human Skin Fibroblasts

1991 ◽  
Vol 19 (12) ◽  
pp. 1057-1065
Author(s):  
Hiroshi YOSHIDA ◽  
Michio SUZUKAWA ◽  
Hiroshi HOSOAI ◽  
Hideki SHIGE ◽  
Kouji TOMIYASU ◽  
...  
Keyword(s):  
Human Skin ◽  
Coenzyme A ◽  
Reductase Activity ◽  
Beta Blockers ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts ◽  
Coenzyme A Reductase
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