A promoter identified in the 3? end of the Ac transposon can be activated by cis-acting elements in transgenic Arabidopsis lines

1996 ◽  
Vol 30 (3) ◽  
pp. 539-551 ◽  
Author(s):  
Sylvie Cocherel ◽  
Pascual Perez ◽  
Fabienne Degroote ◽  
Simonne Genestier ◽  
Georges Picard
Keyword(s):  
Transgenic Arabidopsis ◽  
Cis Acting ◽  
Ac Transposon

scholarly journals Expression of a NGATHA1 Gene from Medicago truncatula Delays Flowering Time and Enhances Stress Tolerance

2020 ◽  
Vol 21 (7) ◽  
pp. 2384
Author(s):  
Tao Guo ◽  
Shumin Wang ◽  
Yinruizhi Li ◽  
Jianbo Yuan ◽  
Lixin Xu ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Stress Tolerance ◽  
Flowering Time ◽  
Medicago Truncatula ◽  
Transgenic Arabidopsis ◽  
Hormone Synthesis ◽  
Cis Acting ◽  
Plant Expression Vector ◽  
Lateral Organ ◽  
Delayed Flowering

Shoot branching is one of the most variable determinants of crop yield, and the signaling pathways of plant branches have become a hot research topic. As an important transcription factor in the B3 family, NGATHA1 (NGA1), plays an important role in regulating plant lateral organ development and hormone synthesis and transport, but few studies of the role of this gene in the regulation of plant growth and stress tolerance have been reported. In this study, the NGA1 gene was isolated from Medicago truncatula (Mt) and its function was characterized. The cis-acting elements upstream of the 5′ end of MtNGA1 and the expression pattern of MtNGA1 were analyzed, and the results indicated that the gene may act as a regulator of stress resistance. A plant expression vector was constructed and transgenic Arabidopsis plants were obtained. Transgenic Arabidopsis showed delayed flowering time and reduced branching phenotypes. Genes involved in the regulation of branching and flowering were differentially expressed in transgenic plants compared with wild-type plants. Furthermore, transgenic plants demonstrated strong tolerances to salt- and mannitol-induced stresses, which may be due to the upregulated expression of NCED3 (NINE-CIS-EPOXYCAROTENOID DIOXYGENASE 3) by the MtNGA1 gene. These results provide useful information for the exploration and genetic modification use of MtNGA1 in the future.

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