Extracellular peroxidases of suspension culture cells of spruce (Picea abies): fungal elicitor-induced inactivation

1994 ◽  
Vol 36 (1) ◽  
pp. 81-90 ◽  
Author(s):  
Burkhard Messner ◽  
Meinrad Boll
Keyword(s):  
Picea Abies ◽  
Suspension Culture ◽  
Fungal Elicitor ◽  
Culture Cells

scholarly journals Effect of Aspergillus flavus Fungal Elicitor on the Production of Terpenoid Indole Alkaloids in Catharanthus roseus Cambial Meristematic Cells

Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3276
Author(s):  
Chuxin Liang ◽  
Chang Chen ◽  
Pengfei Zhou ◽  
Lv Xu ◽  
Jianhua Zhu ◽  
...  
Keyword(s):  
Suspension Culture ◽  
Aspergillus Flavus ◽  
Catharanthus Roseus ◽  
Indole Alkaloids ◽  
Fungal Elicitor ◽  
Control Group ◽  
Terpenoid Indole Alkaloids ◽  
Meristematic Cells ◽  
Qrt Pcr ◽  
Induction Process

This study reported the inducing effect of Aspergillus flavus fungal elicitor on biosynthesis of terpenoid indole alkaloids (TIAs) in Catharanthus roseus cambial meristematic cells (CMCs) and its inducing mechanism. According to the results determined by HPLC and HPLC-MS/MS, the optimal condition of the A. flavus elicitor was as follows: after suspension culture of C. roseus CMCs for 6 day, 25 mg/L A. flavus mycelium elicitor were added, and the CMC suspensions were further cultured for another 48 h. In this condition, the contents of vindoline, catharanthine, and ajmaline were 1.45-, 3.29-, and 2.14-times as high as those of the control group, respectively. Transcriptome analysis showed that D4H, G10H, GES, IRS, LAMT, SGD, STR, TDC, and ORCA3 were involved in the regulation of this induction process. The results of qRT-PCR indicated that the increasing accumulations of vindoline, catharanthine, and ajmaline in C. roseus CMCs were correlated with the increasing expression of the above genes. Therefore, A. flavus fungal elicitor could enhance the TIA production of C. roseus CMCs, which might be used as an alternative biotechnological resource for obtaining bioactive alkaloids.

Simple and efficient plastid transformation system for the liverwort Marchantia polymorpha L. suspension-culture cells

2006 ◽  
Vol 16 (1) ◽  
pp. 41-49 ◽  
Author(s):  
Shota Chiyoda ◽  
Philip J. Linley ◽  
Katsuyuki T. Yamato ◽  
Hideya Fukuzawa ◽  
Akiho Yokota ◽  
...  
Keyword(s):  
Suspension Culture ◽  
Plastid Transformation ◽  
Marchantia Polymorpha ◽  
Transformation System ◽  
Culture Cells

scholarly journals Characterization of Suspension Cultures of Arabidopsis thaliana (L.) Heynh Cells with Altered Expression of the Gene of Alternative Mitochondrial Oxidase Aox1a and Analysis of their Frost Resistance

2021 ◽  
Vol 35 ◽  
pp. 3-18
Author(s):  
A. V. Stepanov ◽  
◽  
S. A. Kashin ◽  
N. S. Zabanova ◽  
O. A. Fedotova ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Suspension Culture ◽  
Mitochondrial Respiration ◽  
Frost Resistance ◽  
Alternative Oxidase ◽  
Uncoupling Protein ◽  
Living Cells ◽  
Subzero Temperature ◽  
Wild Type ◽  
Culture Cells

The enzyme alternative cyanide-resistant oxidase (AOX) localized in mitochondria is involved in the processes of plant adaptation to various unfavorable biotic and abiotic factors. Transfer of electrons from ubiquinone to oxygen by alternative oxidase has a nonprotonmotive character and, by bypassing two sites of H+ pumping in complexes III and IV, lowers the energy efficiency of respiration and energy of electron flow through AOX is released as heat. In this work, we characterized heterotrophic suspension cultures of Arabidopsis thaliana (L.) Heynh cells obtained from seeds of plants with altered (reduced (AS-12 line) and increased (XX-2 line)) expression of the alternative oxidase gene AOX1a and studied their viability under subzero temperature (-10 °С for 3, 6, 9 hours). Cell viability and reactive oxygen species (ROS) production were assessed using fluorescence microscopy with fluorescein diacetate (FDA) and propidium iodide (PI) for cell viability measurement and H2DCF-DA for ROS measurement. The proportion of living cells was calculated as the proportion of FDApositive and PI-negative cells. Differences between the studied lines were determined in the content of mitochondrial proteins of the respiratory chain (AOX, COXII, NDB) and uncoupling protein (UCP), as well as in the intensity of formation of ROS and frost resistance. The obtained results confirmed the higher content of the AOX protein and its high contribution to mitochondrial respiration in line XX-2. Suspension culture cells of the AS-12 line showed a decrease in the AOX protein content and its contribution to mitochondrial respiration, compared to the wild type (Col-0) and line XX-2. Simultaneously with a decrease in the AOX protein content in the AS-12 cell culture, an increase in the content of the uncoupling protein UCP and subunit II of cytochrome oxidase (COXII) was observed. ROS generation was reduced in cell cultures of both XX-2 and AS-12. The obtained results indicate that the cells of the wildtype (Col-0) suspension culture were subjected to the most significant effect of subzero temperature. Long-term exposure (for 9 h) under -10 °С revealed significant differences in the viability of wild-type culture cells and lines with altered AOX1a gene expression. Cells of line XX-2 with an increased content of AOX turned out to be more resistant to subzero temperature compared to wild-type and AS-12 cells. However, while the proportion of living cells in the culture of the AS-12 line 48 h after exposure remained at the same level as immediately after it, in the suspension culture of the wild type cell death developed over time. The obtained results indicate the importance of alternative oxidase in the development of frost resistance in plant cell.

Oleosomes (Spherosomes) from Daucus carota suspension culture cells

Planta ◽  
1978 ◽  
Vol 140 (3) ◽  
pp. 233-237 ◽  
Author(s):  
Hans Kleinig ◽  
Christiane Steinki ◽  
Cornelia Kopp ◽  
Kurt Zaar
Keyword(s):  
Suspension Culture ◽  
Daucus Carota ◽  
Culture Cells
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