Comparison of somatic embryogenesis and embryo conversion in commercial soybean cultivars

1996 ◽  
Vol 44 (1) ◽  
pp. 87-89 ◽  
Author(s):  
Jia Li ◽  
Elizabeth A. Grabau
Keyword(s):  
Somatic Embryogenesis ◽  
Embryo Conversion ◽  
Soybean Cultivars

scholarly journals Evaluation of somatic embryogenesis from immature cotyledons of Japanese soybean cultivars

2007 ◽  
Vol 24 (4) ◽  
pp. 435-440 ◽  
Author(s):  
Susumu Hiraga ◽  
Hiroshi Minakawa ◽  
Koji Takahashi ◽  
Ryoji Takahashi ◽  
Makita Hajika ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Soybean Cultivars ◽  
Immature Cotyledons

scholarly journals Interactions in the Agrobacterium-soybean system and capability of some Brazilian soybean cultivars to produce somatic embryos

2000 ◽  
Vol 23 (1) ◽  
pp. 217-220 ◽  
Author(s):  
Antonio Orlando Di Mauro ◽  
José Carlos Martins de Nóbrega ◽  
Sonia Marli Z. Di Mauro ◽  
Glenn Burton Collins
Keyword(s):  
Somatic Embryogenesis ◽  
Agrobacterium Tumefaciens ◽  
Somatic Embryos ◽  
Significant Interaction ◽  
Virulent Strain ◽  
Soybean Cultivars ◽  
Immature Cotyledons ◽  
Petri Dishes ◽  
Agrobacterium Tumefaciens C58 ◽  
Embryogenic Tissues

Twenty-five Brazilian soybean cultivars were studied for susceptibility to four strains of Agrobacterium tumefaciens (C58, Ach5, Bo542 and A281) and for their ability to produce somatic embryos. Twelve plants of each cultivar were inoculated in a greenhouse at 4-6 weeks of age, using 12 inoculation sites per plant. The number of galls formed on plants were counted 8-10 weeks after inoculation. To study ability to produce somatic embryos, immature cotyledons, 4-6 mm in length, were plated onto N10 medium for induction of somatic embryogenesis, using four Petri dishes with 20 cotyledons for each cultivar. The embryogenic tissues were transferred onto new N10 medium six times at 15-day intervals and the number of somatic embryos per cultivar determined. Significant interaction between soybean cultivars and A. tumefaciens strains was observed; the most virulent strain was A281. The opine type apparently had no effect on strain virulence, and the most embryogenic cultivars were IAS-5, Cristalina, FT-Cometa, IAC-7 and OC-3.

scholarly journals Somatic Embryogenesis and Plant Regeneration from Commercial Soybean Cultivars

Plants ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 38 ◽  
Author(s):  
Ghulam Raza ◽  
Mohan B. Singh ◽  
Prem L. Bhalla
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Somatic Embryo ◽  
Somatic Embryos ◽  
In Vitro Regeneration ◽  
Crop Improvement ◽  
Soybean Cultivars ◽  
Immature Cotyledons ◽  
Soybean Genotypes

The efficient regeneration of plants from commercial genotypes is a pre-requisite for successful genetic transformation, to apply modern crop improvement techniques such as CRISPR-based genome editing. Plant regeneration through the somatic embryogenesis pathway offers an advantage over the organogenesis approach, avoiding the risk of developing chimeras. Plant genotype, explant type, and media compositions play an essential role in the in-vitro regeneration of plants. This study aimed to characterize the commercially grown Australian soybean genotypes for their potential to induce somatic embryos, embryo proliferation, maturation, germination, and plant regeneration. Overall, nine soybean cultivars belonging to different maturity groups were evaluated. Immature cotyledon ranging from 2–4 and 4–6 mm in size were used as explants for somatic embryogenesis induction. Maximum somatic embryo induction frequency (86%) was observed from 4–6 mm immature cotyledons of the cv. Jack (MG III), followed by 66%, 26%, 21%, and 6% in cultivars Williams (MG III), Snowy (MG III), MoonB1 (MG V), and PNR791 (MG V), respectively. On the other hand, cv. Snowy showed maximum somatic-embryo-inducing potential (67%) in 2–4 mm immature cotyledons followed by Williams, Jack, MoonB1, and PNR791. Somatic embryos from Jack, Williams, and Snowy cultivars were further tested for embryo proliferation, maturation, and germination. Maximum proliferation and maturation were observed in cv. Jack, followed by Snowy and Williams. However, cv. Snowy showed a significantly higher conversion of cotyledonary stage embryos to plantlets (85%), than both Jack and Williams cultivars (53% each). In conclusion, this study outlined a protocol for somatic embryogenesis and plant regeneration from three soybean cultivars. Our findings suggest commercial cv. Snowy could be a good candidate for developing transgenic plants through somatic embryogenesis.

Somatic embryogenesis of Cyclamen persicum in liquid medium

1995 ◽  
Vol 94 (4) ◽  
pp. 605-612 ◽  
Author(s):  
Marc Kreuger ◽  
Erik Postma ◽  
Yvon Brouwer ◽  
Gerrit-Jan van Holst
Keyword(s):  
Somatic Embryogenesis ◽  
Liquid Medium ◽  
Cyclamen Persicum

Somatic Embryogenesis and In vitro Plant Regeneration in Vigna trilobata (L.) Verd. - A Potential Pasture Legume

1970 ◽  
Vol 19 (1) ◽  
pp. 89-99
Author(s):  
K. Choudhary ◽  
M. Singh ◽  
M. S. Rathore ◽  
N. S. Shekhawat
Keyword(s):  
Somatic Embryogenesis ◽  
Growth Regulators ◽  
Somatic Embryos ◽  
Basal Medium ◽  
Long Term Study ◽  
Continuous Application ◽  
First Time ◽  
Pasture Legume

This long term study demonstrates for the first time that it is possible to propagate embryogenic Vigna trilobata and to subsequently initiate the differentiation of embryos into complete plantlets. Initiation of callus was possible on 2,4-D. Somatic embryos differentiated on modified MS basal nutrient medium with 1.0 mg/l  of 2,4-D and 0.5 mg/l  of Kn. Sustained cell division resulted in globular and heart shape stages of somatic embryos. Transfer of embryos on to a fresh modified MS basal medium with 0.5 mg/l of Kn and 0.5 mg/l of GA3 helped them to attain maturation and germination. However, the propagation of cells, as well as the differentiation of embryos, were inhibited by a continuous application of these growth regulators. For this reason, a long period on medium lacking these growth regulators was necessary before the differentiation of embryos occurred again. The consequences for improving the propagation of embryogenic cultures in Vigna species are discussed. Key words: Pasture  legume, Vigna trilobata, Globular, Heart shape, somatic embryogenesis D.O.I. 10.3329/ptcb.v19i1.4990 Plant Tissue Cult. & Biotech. 19(1): 89-99, 2009 (June)

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