In vitro culture of Cucumis sativus L. XVIII. Plants from protoplasts through direct somatic embryogenesis

1995 ◽  
Vol 41 (3) ◽  
pp. 259-266 ◽  
Author(s):  
W. Burza ◽  
S. Malepszy
Keyword(s):  
Somatic Embryogenesis ◽  
In Vitro Culture ◽  
Cucumis Sativus ◽  
Direct Somatic Embryogenesis ◽  
Cucumis Sativus L

scholarly journals Somatic embryogenesis in in vitro culture of Plantago asiatica L.

2014 ◽  
Vol 69 (4) ◽  
pp. 245-250 ◽  
Author(s):  
Joanna Makowczyńska ◽  
Emilia Andrzejewska-Golec
Keyword(s):  
Somatic Embryogenesis ◽  
Light Microscopy ◽  
In Vitro Culture ◽  
Direct Somatic Embryogenesis ◽  
Early Stages ◽  
Plantago Asiatica ◽  
Somatic Organogenesis

Early stages of direct somatic embryogenesis in <em>Plantago asiatica</em> were observed by light microscopy. In calli of <em>Plantago asiatica</em> two phenomena occurred simultaneously: somatic organogenesis and somatic embryogenesis.

scholarly journals Cytohistological analysis of somatic embryogenesis in cucumber (Cucumis sativus L). II. Natural fluorescence and direct somatic embryogenesis from protoplasts

2014 ◽  
Vol 63 (3-4) ◽  
pp. 265-268 ◽  
Author(s):  
W. Burza ◽  
B. Woźniak ◽  
J. A. Tarkowska ◽  
S. Malepszy
Keyword(s):  
Somatic Embryogenesis ◽  
Protoplast Culture ◽  
Direct Somatic Embryogenesis ◽  
Histological Structure ◽  
Cucumis Sativus L ◽  
Natural Fluorescence ◽  
Vacuolated Cells ◽  
Selection Of

The development of protoplast derived from somatic embryos and some of their characteristics were compared with embryos from suspension and in vivo in the same B line. Embryos formed in a protoplast culture differed from others that their younger stages contained vacuolated cells, and older ones had altered morphological and histological structure. Somatic embryogenesis is more regular from suspension then from protoplasts. No distinct differences were observed in the rate of embryo development in vivo and in vitro, and in vitro embryos show a larger variation in size at the same stage. Embryos in vitro with fluorescence are generally larger than zygotic ones at each stage. The use of fluorescence is suggested for the selection of heterokariocytes after protoplast fusion.

In vitro culture of Cucumis sativus L.

1989 ◽  
Vol 78 (6) ◽  
pp. 836-840 ◽  
Author(s):  
A. Nadolska-Orczyk ◽  
S. Malepszy
Keyword(s):  
In Vitro Culture ◽  
Cucumis Sativus ◽  
Cucumis Sativus L

scholarly journals In vitro culture of Cucumis sativus L. VI. Histological analysis of leaf explants cultured on media with 2, 4-D or 2, 4, 5-T

2014 ◽  
Vol 56 (1) ◽  
pp. 55-60 ◽  
Author(s):  
Anna Nadolska-Orczyk ◽  
Stefan Malepszy
Keyword(s):  
Somatic Embryogenesis ◽  
Cucumis Sativus ◽  
Histological Analysis ◽  
Leaf Explants ◽  
Vascular Bundles ◽  
Meristematic Tissue ◽  
Cucumis Sativus L ◽  
Meristematic Cells ◽  
Cellular Aggregates

The developmental sequence of callus initiation and somatic embryogenesis in leaf explants of <em>Cucumis sativus</em> cv. Borszczagowski was analysed and compared on media containing two different auxin phenoxy-derivatives (2,4-D and 2,4,5-T) and cytokinin (BAP or 2iP). During the first 20 days of culture on media with 2,4,5-T proliferation of parenchymatic tissue occurred mainly and only small meristematic centers were observed. There was an intensive detachment of parenchymatic cells and dissociation of their cell walls near vessels and in the lower part of the explant adjacent to the medium. These cells were strongly plasmolysed. On the 2,4-D containing medium mostly meristematic tissue developed, proliferating around vascular bundles and forming meristematic centers or promeristem-like structures. After 35-50 days of culture, secondary callus was formed by separation of meristematic cells from the meristem surface in explants cultured on the 2,4-D containing medium. On medium supplemented with 2, 4, 5-T the detachment of parenchymatic and meristematic cells occurred, along with formation of a gel-like substance. The gel-like callus contained multi-cellular aggregates, proembryoids and embryoids. This type of callus tissue was initiated more intensively on medium with 2, 4, 5-T, but the frequency of somatic embryogenesis was much lower. The periferial cells of aggregates, proembryoids and embryoids showed the tendency to separate from the surface of the tissue. Many embryoids formed adventitious embryos.

Effect of Ethylene Inhibitors on in vitro Shoot Multiplication and their Impact on Ethylene Production in Cucumber (Cucumis sativus L.)

2006 ◽  
Vol 1 (3) ◽  
pp. 110-117 ◽  
Author(s):  
Vasudevan Ayyappan . ◽  
Selvaraj Natesan . ◽  
Ganapathi Andy . ◽  
Chang won Choi . ◽  
Manickavasagam Marka . ◽  
...  
Keyword(s):  
Cucumis Sativus ◽  
Ethylene Production ◽  
Shoot Multiplication ◽  
Ethylene Inhibitors ◽  
Cucumis Sativus L

IN VITRO MORPHOGENESIS OF SHOOTS FROM LEAF EXPLANTS OF CUCUMBER (CUCUMIS SATIVUS L.)

2006 ◽  
pp. 155-160 ◽  
Author(s):  
N. Selvarai ◽  
A. Ganapath ◽  
A. Vasudevan ◽  
G. Vengadesan ◽  
S. Kasthuri Rengan
Keyword(s):  
Cucumis Sativus ◽  
Leaf Explants ◽  
In Vitro Morphogenesis ◽  
Cucumis Sativus L
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