Isolation and characterization of an unusual repeated sequence from the ribosomal intergenic spacer of the crucifer Sisymbrium irio

1989 ◽  
Vol 12 (6) ◽  
pp. 695-706 ◽  
Author(s):  
Fran�oise Grellet ◽  
Dominique Delcasso-Tremousaygue ◽  
Michel Delseny
Keyword(s):  
Intergenic Spacer ◽  
Repeated Sequence ◽  
Isolation And Characterization ◽  
Ribosomal Intergenic Spacer ◽  
Sisymbrium Irio

scholarly journals Isolation and characterization of a repeated sequence (RPS1) of Candida albicans

1992 ◽  
Vol 138 (9) ◽  
pp. 1893-1900 ◽  
Author(s):  
S.-I. Iwaguchi ◽  
M. Homma ◽  
H. Chibana ◽  
K. Tanaka
Keyword(s):  
Candida Albicans ◽  
Repeated Sequence ◽  
Isolation And Characterization

scholarly journals Application of Ribosomal Intergenic Spacer Analysis to the Characterization of Microbial Communities in Rhizospheric Soils of the Bt Transgenic Chrysanthemum

2005 ◽  
Vol 20 (4) ◽  
pp. 258-263 ◽  
Author(s):  
Seishi Ikeda ◽  
Harue Shinoyama ◽  
Shinsuke Yamanaka ◽  
Nozomi Ytow ◽  
Hiroshi Ezura ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Intergenic Spacer ◽  
Ribosomal Intergenic Spacer ◽  
Transgenic Chrysanthemum

scholarly journals Comparative Characterization of the Microbial Diversities of an Artificial Microbialite Model and a Natural Stromatolite

2008 ◽  
Vol 74 (23) ◽  
pp. 7410-7421 ◽  
Author(s):  
Stephanie A. Havemann ◽  
Jamie S. Foster
Keyword(s):  
Calcium Carbonate ◽  
Gene Sequencing ◽  
Intergenic Spacer ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Filamentous Cyanobacteria ◽  
Ribosomal Intergenic Spacer ◽  
Bacterial Clone ◽  
Rrna Gene Sequencing

ABSTRACT Microbialites are organosedimentary structures that result from the trapping, binding, and lithification of sediments by microbial mat communities. In this study we developed a model artificial microbialite system derived from natural stromatolites, a type of microbialite, collected from Exuma Sound, Bahamas. We demonstrated that the morphology of the artificial microbialite was consistent with that of the natural system in that there was a multilayer community with a pronounced biofilm on the surface, a concentrated layer of filamentous cyanobacteria in the top 5 mm, and a lithified layer of fused oolitic sand grains in the subsurface. The fused grain layer was comprised predominantly of the calcium carbonate polymorph aragonite, which corresponded to the composition of the Bahamian stromatolites. The microbial diversity of the artificial microbialites and that of natural stromatolites were also compared using automated ribosomal intergenic spacer analysis (ARISA) and 16S rRNA gene sequencing. The ARISA profiling indicated that the Shannon indices of the two communities were comparable and that the overall diversity was not significantly lower in the artificial microbialite model. Bacterial clone libraries generated from each of the three artificial microbialite layers and natural stromatolites indicated that the cyanobacterial and crust layers most closely resembled the ecotypes detected in the natural stromatolites and were dominated by Proteobacteria and Cyanobacteria. We propose that such model artificial microbialites can serve as experimental analogues for natural stromatolites.

Isolation and characterization of an ?-satellite repeated sequence from human chromosome 22

Chromosoma ◽  
1986 ◽  
Vol 94 (3) ◽  
pp. 228-234 ◽  
Author(s):  
Heather E. McDermid ◽  
Alessandra M. V. Duncan ◽  
Michael J. Higgins ◽  
John L. Hamerton ◽  
Edward Rector ◽  
...  
Keyword(s):  
Human Chromosome ◽  
Repeated Sequence ◽  
Chromosome 22 ◽  
Isolation And Characterization

scholarly journals Ribosomal Intergenic Spacer 1 Based Characterization of Button Mushroom (Agaricus bisporus) Strains

Mycobiology ◽  
2016 ◽  
Vol 44 (4) ◽  
pp. 314-318 ◽  
Author(s):  
Hyuk Woo Kwon ◽  
Min Ah Choi ◽  
Dae Wook Kim ◽  
Youn-Lee Oh ◽  
Min Woo Hyun ◽  
...  
Keyword(s):  
Agaricus Bisporus ◽  
Intergenic Spacer ◽  
Button Mushroom ◽  
Ribosomal Intergenic Spacer

Characterization of natural populations of Nitrobacter spp. using PCR/RFLP analysis of the ribosomal intergenic spacer

1992 ◽  
Vol 157 (2) ◽  
pp. 107-115 ◽  
Author(s):  
Elisabeth Navarro ◽  
Pascal Simonet ◽  
Philippe Normand ◽  
René Bardin
Keyword(s):  
Natural Populations ◽  
Intergenic Spacer ◽  
Rflp Analysis ◽  
Ribosomal Intergenic Spacer ◽  
Pcr Rflp

scholarly journals Cultivation-Independent Characterization of Methylobacterium Populations in the Plant Phyllosphere by Automated Ribosomal Intergenic Spacer Analysis

2008 ◽  
Vol 74 (7) ◽  
pp. 2218-2228 ◽  
Author(s):  
Claudia Knief ◽  
Lisa Frances ◽  
Franck Cantet ◽  
Julia A. Vorholt
Keyword(s):  
Intergenic Spacer ◽  
23S Rrna ◽  
Rrna Gene ◽  
Length Variation ◽  
Ribosomal Intergenic Spacer ◽  
Pcr Products ◽  
23S Rrna Gene ◽  
Reverse Primer ◽  
Insight Into

ABSTRACT Bacteria of the genus Methylobacterium are widespread in the environment, but their ecological role in ecosystems, such as the plant phyllosphere, is not very well understood. To gain better insight into the distribution of different Methylobacterium species in diverse ecosystems, a rapid and specific cultivation-independent method for detection of these organisms and analysis of their community structure is needed. Therefore, 16S rRNA gene-targeted primers specific for this genus were designed and evaluated. These primers were used in PCR in combination with a reverse primer that binds to the tRNAAla gene, which is located upstream of the 23S rRNA gene in the 16S-23S intergenic spacer (IGS). PCR products that were of different lengths were obtained due to the length heterogeneity of the IGS of different Methylobacterium species. This length variation allowed generation of fingerprints of Methylobacterium communities in environmental samples by automated ribosomal intergenic spacer analysis. The Methylobacterium communities on leaves of different plant species in a natural field were compared using this method. The new method allows rapid comparisons of Methylobacterium communities and is thus a useful tool to study Methylobacterium communities in different ecosystems.

The isolation and characterization of 18 equine microsatellite loci, TKY272–TKY289

2000 ◽  
Vol 31 (2) ◽  
pp. 149-149 ◽  
Author(s):  
T Tozaki ◽  
H Kakoi ◽  
S Mashima ◽  
K Hirota ◽  
T Hasegawa ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Isolation And Characterization
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